Gustavo H. C. Varca 2014 Nuclear and Energy Research Institute IPEN- CNEN/SP BRAZIL

Size: px
Start display at page:

Download "Gustavo H. C. Varca 2014 Nuclear and Energy Research Institute IPEN- CNEN/SP BRAZIL"

Transcription

1 SYNTHESIS OF PAPAIN NANOPARTICLES USING E- BEAM AND GAMMA IRRADIATION: A RADIATION CHEMISTRY APPROACH Gustavo H. C. Varca 2014 Nuclear and Energy Research Institute IPEN- CNEN/SP BRAZIL

2 Protein- Based Nanoparticles Overview

3 Protein in Pharmaceutics ü Drug delivery systems ü Tissue engineering (scaffolds) ü Therapeutical peptides ü Protein pharmaceutics

4 Protein Based Drug Carrier Protein NPs cell recognition v Specific receptors in the cell membrane Site specific delivery v Specific orientation to interact with receptor Similar conformation v Nanoparticle- bound protein/free protein competition for the receptor Affinity Modifications Lynch et al., Nature Nanotechnology 4, (2009)

5 Radiation Synthesized Protein- Based Nanoparticles Technique Overview

6 Particle Synthesis Technique Overview Step 1 Step 2 Step 3 - Concentration - ph - Cosolvents [ ] - Temperature - Dosis - Irradiation temp. Varca et al. Radiat. Phys. Chem. (2014a); BR Patent /03/2013

7 Controlled Nanostructure Drug Carrier Covalent Attachment More stable structure Caused Structural rearrangement Biological properties Drug carrier - potential - Bioactive - Controllable size distr. - No use of monomers Varca et al. Radiat. Phys. Chem. 2014(a); BR Patent /03/2013

8 Radiation Synthesized Protein- Based Nanoparticles The case of Papain

9 Papain Figure 1. Papain structure (frontal view). Structural properties amino acids (2 domains) - Active site - Asn, Cis and His Ø Cysteine protease Broad specificity Carica Papaya L. (EC ) Thiol protease Ø Applications Biotechnology esterase amidase Industrial level endopeptidase Biomedical (Rosenberg, et al., 2004; Kamphuis et al., 1984)

10 Particle Size Increment γ irradiation Size (d.nm) mg.ml-1 20mg.ml-1 50mg.ml % 10% 20% 30% 35% Ethanol% Figure 1. Scale up experiments involving different papain (12.5, 20 and 50 mg.ml -1 ) and ethanol (0-35% v/v) concentrations irradiated at 10 kgy. Size (d.nm) 10,5 20% EtOH 10,0 0% EtOH 9,5 9,0 8,5 8,0 7,5 7,0 6,5 6,0 5,5 5,0 4,5 0,0 2,5 5,0 7,5 10,0 Radiation dose (kgy) Figure 2. Papain particle size increment as a function of irradiation dose in presence (20% v/v) and absence of ethanol. Controlled Particle Size increase Varca et al. Radiat. Phys. Chem. (2014B);

11 Effect of γ- irradiation Non irradiated samples Agglomeration Irradiated Samples Agglomeration Size increase Van der Waals/Electrostatic forces/ dipole- dipole Size increase Crosslinking (intermolecular) Covalent attachment 0% EtOH 30% EtOH 40% EtOH Dilution 30% EtOH 40% EtOH

12 Mechanism of NP Formation Ø Direct effects - Minimum Indirect effects Irradiation in presence of L- Cysteine No particle formation Oxidizing radical species 0% EtOH 30% EtOH

13 Mechanism Approach - Bityrosine A B Figure 3. Bityrosine monitoring in papain (12.5 mg.ml -1 ) non-irradiated (a) and irradiated (b) at 10kGy under different ethanol concentrations (0-35%). The samples were properly diluted in buffer to reach equivalent absorbance. The readings were performed at λ=280nm using a Cary 1E Uv-Vis Varian spectrophotometer. The samples were then checked for bityrosine emission on a F4500 Hitachi Fluorescence spectrophotometer using λex=325nm λem= nm, scan speed of 240nm/min, Exslit of 5nm and Emslit of 10nm. Controlled Bityrosine Crosslinking Induced by Radiation Varca et al. Radiat. Phys. Chem. (2014B);

14 E- Beam - Radiation Chemistry Overview Absorbance units (a.u.) 0,010 0,009 0,008 0,007 0,006 0,005 0,004 0,003 0, Wavelenght (nm) Figure 4. UV-Vis absorption spectra of OH -papain adduct determined by pulse radiolysis. N 2 O saturated aqueous papain solution ( mol dm -3 ) irradiated with 7 ns electron pulses. Dose per pulse 20 Gy. UV-Vis absorbance recorded at wavelength ranging from λ = 290 to 500 nm. k' OH [1/s] 7E6 6E6 5E6 4E6 3E6 2E6 1,5E-5 2,0E-5 2,5E-5 3,0E-5 3,5E-5 4,0E-5 4,5E-5 Papain concentration [mol dm -3 ] Figure 5. Determination of papain and OH radical reaction rate constant. Pulse radiolysis of papain (concentrations ranging from to mol dm -3 ) in N 2 O saturated aqueous solution. Pulse duration 7 ns, dose per pulse 20Gy. UV-Vis absorbance recorded at 330 nm. OH (dm - 3 mol - 1 s - 1 )

15 E- Beam - Radiation Chemistry Overview 4,5E6 k'e [1/s] 4,0E6 e (dm - 3 mol - 1 s - 1 ) 3,5E6 1,0E-5 1,5E-5 2,0E-5 2,5E-5 3,0E-5 3,5E-5 4,0E-5 4,5E-5 Papain Concentration (mol dm -3 ) Figure 6. UV-Vis absorption spectra of OH -papain adduct determined by pulse radiolysis. N 2 O saturated aqueous papain solution ( mol dm -3 ) irradiated with 7 ns electron pulses. Dose per pulse 20 Gy. UV-Vis absorbance recorded at wavelength ranging from λ = 290 to 500 nm.

16 E- Beam - Radiation Chemistry Overview 7,0E-4 6,0E-4 t 1/2 5,0E-4 4,0E-4 3,0E-4 2,0E-4 This indicates that even for 10% (w/w) ethanol solution OH radicals are not scavenged by alcohol and are still able to interact with papain 1,0E-4 0 1E-5 2E-5 3E-5 4E-5 5E-5 Papain Concentration (mol dm -3 ) Figure 7. Competitive kinetics of papain and ethanol with hydroxyl radicals. Pulse radiolysis of papain (concentrations ranging from 0 up to mol dm -3 ) in N 2 O saturated aqueous solution with addition of ethanol 10% (w/w). Pulse duration 7 ns, dose per pulse 20Gy. UV-Vis absorbance recorded at 330 nm.

17 Conclusions Ø The synthesis of protein NP s with controllable size distribution was achieved by gamma and electron; Ø The OH. is more likely to be involved in the process of NP Formation / Bityrosine formation; Ø There are still papain molecules interacting with OH. At higher ethanol concentrations;

18 Acknowledgments ü Piotr Ulansky (IMTR- Lodz, PL) ü Janus Rosiak (IMTR- Lodz, PL) ü Slawomir Kadlubousky (IMTR- Lodz, PL) ü Mariano Grasselli (UNQ Buenos Aires, AR) ü Ademar B. Lugão (IPEN- CNEN/SP, Brazill) Process number 2010/

19 Thanks for your attention! Visit our website!!!

Metal Nanoparticles Stabilized by Organic Ligands. Priyanka Ray, Cyril Martini, Vincent Huc, Isabelle Lampre, Hynd Remita

Metal Nanoparticles Stabilized by Organic Ligands. Priyanka Ray, Cyril Martini, Vincent Huc, Isabelle Lampre, Hynd Remita Metal Nanoparticles Stabilized by Organic Ligands Priyanka Ray, Cyril Martini, Vincent Huc, Isabelle Lampre, Hynd Remita Radiolytic synthesis of metal clusters H 2 O g, e - e s-, H 3 O +, H, OH, H 2, H

More information

Drug delivery glucantime in PVP/chitosan membranes

Drug delivery glucantime in PVP/chitosan membranes 2015 International Nuclear Atlantic Conference - INAC 2015 São Paulo, SP, Brazil, October 4-9, 2015 ASSOCIAÇÃO BRASILEIRA DE ENERGIA NUCLEAR - ABEN ISBN: 978-85-99141-06-9 Drug delivery glucantime in PVP/chitosan

More information

ELEMENTARY RADIATION CHEMISTRY

ELEMENTARY RADIATION CHEMISTRY ELEMENTARY RADIATION CEMISTRY RADIOLYSIS The overall process of forming chemically stable products after the absorption and redistribution of the excess of energy of ionizing radiation The resulting compounds

More information

Interaction of Gold Nanoparticle with Proteins

Interaction of Gold Nanoparticle with Proteins Chapter 7 Interaction of Gold Nanoparticle with Proteins 7.1. Introduction The interfacing of nanoparticle with biomolecules such as protein is useful for applications ranging from nano-biotechnology (molecular

More information

Chapter 5: Radiation induced synthesis of anisotropic gold nanoparticles and their characterization

Chapter 5: Radiation induced synthesis of anisotropic gold nanoparticles and their characterization Chapter 5: Radiation induced synthesis of anisotropic gold nanoparticles and their characterization 5.1. Introduction Fine metal particles with nanometer scale dimensions are of current interest due to

More information

Ultrafast electron transfer studied by picosecond pulse radiolysis, new redox reactions. Mehran Mostafavi, April 2017, Vienna, Austria H 2 O * H 2 O

Ultrafast electron transfer studied by picosecond pulse radiolysis, new redox reactions. Mehran Mostafavi, April 2017, Vienna, Austria H 2 O * H 2 O Ultrafast electron transfer studied by picosecond pulse radiolysis, new redox reactions Mehran Mostafavi, April 2017, Vienna, Austria e hyd H 3 O + H 2 O * H 2 O H 2 O H 2 O + S e 1 Mostafavi, AIEA, April

More information

Hybrid Gold Superstructures: Synthesis and. Specific Cell Surface Protein Imaging Applications

Hybrid Gold Superstructures: Synthesis and. Specific Cell Surface Protein Imaging Applications Supporting Information Hybrid Gold Nanocube@Silica@Graphene-Quantum-Dot Superstructures: Synthesis and Specific Cell Surface Protein Imaging Applications Liu Deng, Ling Liu, Chengzhou Zhu, Dan Li and Shaojun

More information

Supplementary information

Supplementary information Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2016 Supplementary information 1. Composition of the nanoparticles 2. Dynamic light scattering 3. Scanning

More information

Effect of Addition Au Nanoparticles on Emission Spectra of Laser Dye

Effect of Addition Au Nanoparticles on Emission Spectra of Laser Dye International Journal of Applied Engineering Research ISSN 973-462 Volume 2, Number 24 (27) pp. 4833-484 Effect of Addition Au Nanoparticles on Emission Spectra of Laser Dye Sara Ali Razzak, Lazem Hassan

More information

Raman spectroscopy of phthalocyanines and their sulfonated derivatives

Raman spectroscopy of phthalocyanines and their sulfonated derivatives Journal of Molecular Structure 744 747 (2005) 481 485 www.elsevier.com/locate/molstruc Raman spectroscopy of phthalocyanines and their sulfonated derivatives B. Brożek-Płuska*, I. Szymczyk, H. Abramczyk

More information

Effects of ã-irradiation on Some Properties of Gum Arabic (Acacia Senegal L)

Effects of ã-irradiation on Some Properties of Gum Arabic (Acacia Senegal L) Research Journal of Agriculture and Biological Sciences, 6(2): 113-117, 2010 2010, INSInet Publication Effects of ã-irradiation on Some Properties of Gum Arabic (Acacia Senegal L) 1 2 Siddig T. Kafi, Murwan.

More information

IRRADIATION EFFECTS ON POLY (VINYL CHLORIDE)

IRRADIATION EFFECTS ON POLY (VINYL CHLORIDE) IRRADIATION EFFECTS ON POLY (VINYL CHLORIDE) L.COSTA, V. BRUNELLA, P. BRACCO Dipartimento di Chimica IFM, Università di Torino, Italy E-mail: brunella@ch.unito.it We have studied the electron beam effects

More information

A Fluorescence Turn-On Sensor for the Detection of Palladium Ions that Operates Through In-Situ Generation of Palladium Nanoparticles

A Fluorescence Turn-On Sensor for the Detection of Palladium Ions that Operates Through In-Situ Generation of Palladium Nanoparticles This journal is The Royal Society of Chemistry 214 Supporting Information for A Fluorescence Turn-On Sensor for the Detection of Palladium Ions that Operates Through In-Situ Generation of Palladium Nanoparticles

More information

CD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray

CD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray CD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray crystallography An example of the use of CD Modeling

More information

UNIT 3 CHEMISTRY. Fundamental Principles in Chemistry

UNIT 3 CHEMISTRY. Fundamental Principles in Chemistry UNIT 3 CHEMISTRY NOTE: This list has been compiled based on the topics covered in the 2016 Master Class program. Once all of the 2017 Chemistry program materials have been finalised, this summary will

More information

CHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK

CHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK 161 CHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK 7.1 SUMMARY OF THE PRESENT WORK Nonlinear optical materials are required in a wide range of important applications, such as optical

More information

Supplementary Information

Supplementary Information Supplementary Information A fluorescent turn-on probe for bisulfite based on hydrogen bond-inhibited C=N isomerization mechanism Yuan-Qiang Sun, Pi Wang, Jing Liu, Jingyu Zhang and Wei Guo* School of Chemistry

More information

Supporting Information. Hydroxyl Radical Attack on Reduced Graphene Oxide

Supporting Information. Hydroxyl Radical Attack on Reduced Graphene Oxide Supporting Information Making Graphene Holey. Gold Nanoparticle-Mediated Hydroxyl Radical Attack on Reduced Graphene Oxide James G. Radich, 1,3 Prashant V. Kamat *1,2,3 Radiation Laboratory Department

More information

Spectroscopy Chapter 13

Spectroscopy Chapter 13 Spectroscopy Chapter 13 Electromagnetic Spectrum Electromagnetic spectrum in terms of wavelength, frequency and Energy c=λν c= speed of light in a vacuum 3x108 m/s v= frequency in Hertz (Hz s-1 ) λ= wavelength

More information

Pulse Radiolysis of Acrylic Acid Ester and Acryamide Type Monomers in Dilute Aqueous and Cyclohexane Solutions

Pulse Radiolysis of Acrylic Acid Ester and Acryamide Type Monomers in Dilute Aqueous and Cyclohexane Solutions PhD Thesis-books Pulse Radiolysis of Acrylic Acid Ester and Acryamide Type Monomers in Dilute Aqueous and Cyclohexane Solutions By Katalin Dajka M. Sc. in Chemical Engineering Supervisor: Erzsébet Takács,

More information

Hydroxyl radical induced degradation of aromatic molecules

Hydroxyl radical induced degradation of aromatic molecules Hydroxyl radical induced degradation of aromatic molecules 1 Poorest source of hydroxyl radical Radiolysis of water H 2 O H 2 O + + e H 2 O H 2 O* H 2 O + + H 2 O H 3 O + + OH e + nh 2 O e aq H 2 O* OH

More information

Poly(N-vinyl-2-pyrrolidone) hydrogel production by ultraviolet radiation: new methodologies to accelerate crosslinking

Poly(N-vinyl-2-pyrrolidone) hydrogel production by ultraviolet radiation: new methodologies to accelerate crosslinking Polymer 45 (2004) 4705 4709 www.elsevier.com/locate/polymer Poly(N-vinyl-2-pyrrolidone) hydrogel production by ultraviolet radiation: new methodologies to accelerate crosslinking G.J.M. Fechine*, J.A.G.

More information

Seminars in Nanosystems - I

Seminars in Nanosystems - I Seminars in Nanosystems - I Winter Semester 2011/2012 Dr. Emanuela Margapoti Emanuela.Margapoti@wsi.tum.de Dr. Gregor Koblmüller Gregor.Koblmueller@wsi.tum.de Seminar Room at ZNN 1 floor Topics of the

More information

MAGNETIC GADOLINIUM-CHITOSAN COMPOSITE NANOPARTICLES CREATED BY RADIOLYTIC SYNTHESIS

MAGNETIC GADOLINIUM-CHITOSAN COMPOSITE NANOPARTICLES CREATED BY RADIOLYTIC SYNTHESIS 18 TH INTERNATIONAL CONFERENCE ON COMPOSITE MATERIALS MAGNETIC GADOLINIUM-CHITOSAN COMPOSITE NANOPARTICLES W. Pasanphan*, L. Chunkoh, S. Choofong Department of Applied Radiation and Isotopes, Faculty of

More information

Detonation Nanodiamond Suspensions

Detonation Nanodiamond Suspensions PRODUCT SHEET Rev. 10/17, v3.0 Detonation Nanodiamond Suspensions Colloidal suspensions of detonation nanodiamond (DND) in both and a variety of organic solvents have a wide range of uses, including: (1)

More information

Photoinduced Guest Transformation Promotes Translocation of Guest from Hydroxypropyl-β-Cyclodextrin to Cucurbit[7]uril

Photoinduced Guest Transformation Promotes Translocation of Guest from Hydroxypropyl-β-Cyclodextrin to Cucurbit[7]uril Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Photoinduced Guest Transformation Promotes Translocation of Guest from Hydroxypropyl-β-Cyclodextrin

More information

An ESR and DFT study of Hydration of the 2 -Deoxyuridine-5-yl

An ESR and DFT study of Hydration of the 2 -Deoxyuridine-5-yl Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information An ESR and DFT study of Hydration of the 2 -Deoxyuridine-5-yl

More information

INVESTIGATIONS OF THE FRICKE GEL (FXG) DOSIMETER DEVELOPED AT IPEN IRRADIATED WITH 60 Co GAMMA RAYS

INVESTIGATIONS OF THE FRICKE GEL (FXG) DOSIMETER DEVELOPED AT IPEN IRRADIATED WITH 60 Co GAMMA RAYS 2007 International Nuclear Atlantic Conference - INAC 2007 Santos, SP, Brazil, September 30 to October 5, 2007 ASSOCIAÇÃO BRASILEIRA DE ENERGIA NUCLEAR - ABEN ISBN: 978-85-99141-02-1 INVESTIGATIONS OF

More information

Supporting Information

Supporting Information Supporting Information Dynamic Interaction between Methylammonium Lead Iodide and TiO 2 Nanocrystals Leads to Enhanced Photocatalytic H 2 Evolution from HI Splitting Xiaomei Wang,, Hong Wang,, Hefeng Zhang,,

More information

Infrared Spectroscopy: Identification of Unknown Substances

Infrared Spectroscopy: Identification of Unknown Substances Infrared Spectroscopy: Identification of Unknown Substances Suppose a white powder is one of the four following molecules. How can they be differentiated? H N N H H H H Na H H H H H A technique that is

More information

STUDY ON THE SYNTHESIS OF COLLOIDAL SILVER ANTIMICROBIAL SUBSTANCE

STUDY ON THE SYNTHESIS OF COLLOIDAL SILVER ANTIMICROBIAL SUBSTANCE VAEC-AR 7--3 STUDY ON THE SYNTHESIS OF COLLOIDAL SILVER NANOPARTICLES BY γ-irradiation FOR USING AS AN ANTIMICROBIAL SUBSTANCE Dang Van Phu (), Bui Duy Du (), Nguyen Trieu (), Vo Thi Kim Lang () Doan Thi

More information

Initiation of nuclear reactions under laser irradiation of Au nanoparticles in the aqueous solution of Uranium salt. A.V. Simakin and G.A.

Initiation of nuclear reactions under laser irradiation of Au nanoparticles in the aqueous solution of Uranium salt. A.V. Simakin and G.A. Initiation of nuclear reactions under laser irradiation of Au nanoparticles in the aqueous solution of Uranium salt A.V. Simakin and G.A. Shafeev Wave Research Center of A.M. Prokhorov General Physics

More information

Synthesis of 2 ) Structures by Small Molecule-Assisted Nucleation for Plasmon-Enhanced Photocatalytic Activity

Synthesis of 2 ) Structures by Small Molecule-Assisted Nucleation for Plasmon-Enhanced Photocatalytic Activity Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Electronic Supplementary Information Synthesis of Au@UiO-66(NH 2 ) Structures by Small Molecule-Assisted

More information

Radiation Processing. Chemical Effects

Radiation Processing. Chemical Effects Radiation Processing Chemical Effects Reactions ofhydrated Electron Major Reactions of e-aq H 2 0 1. e- aq + N 2 0 N 2 + {)H Used to detect/estimate e- aq and to increase OH yield 2. e- aq + O 2 2-...

More information

Radiation Induced Reduction: A Effect and Clean Route to

Radiation Induced Reduction: A Effect and Clean Route to Supporting Information for Radiation Induced Reduction: A Effect and Clean Route to Synthesize Functionalized Graphene Bowu ZHANG, a, b Linfan LI, a Ziqiang WANG, a Siyuan XIE, a, b Yujie ZHANG, c Yue

More information

Electronic Supplementary Information

Electronic Supplementary Information Electronic Supplementary Information Formation of MS-Ag and MS (M=Pb, Cd, Zn) nanotubes via microwave-assisted cation exchange and their enhanced photocatalytic activities Yanrong Wang, a Wenlong Yang,

More information

SUPPORTING INFORMATION

SUPPORTING INFORMATION Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2016 SUPPORTING INFORMATION Synthesis, Characterization and Biological Activity of Fluorescently

More information

Supporting Information s for

Supporting Information s for Supporting Information s for # Self-assembling of DNA-templated Au Nanoparticles into Nanowires and their enhanced SERS and Catalytic Applications Subrata Kundu* and M. Jayachandran Electrochemical Materials

More information

Chem 155 Quiz 3 Review Topics: Quiz 3 outline

Chem 155 Quiz 3 Review Topics: Quiz 3 outline Quiz 3 outline 1. Atomic absorption spectrometry a. Principles of FAAS where selectivity and sensitivity arise b. Spectrometer design c. Atomization processes d. Sensitvitiy and atomization: protecting

More information

UV-vis Analysis of the Effect of Sodium Citrate on the Size and the Surface Plasmon Resonance of Au NPs. Eman Mousa Alhajji

UV-vis Analysis of the Effect of Sodium Citrate on the Size and the Surface Plasmon Resonance of Au NPs. Eman Mousa Alhajji UV-vis Analysis of the Effect of Sodium Citrate on the Size and the Surface Plasmon Resonance of Au NPs Eman Mousa Alhajji North Carolina State University Department of Materials Science and Engineering

More information

Preparation of Gold Nanoparticles by Pulsed Laser Ablation in NaOH solution

Preparation of Gold Nanoparticles by Pulsed Laser Ablation in NaOH solution Preparation of Gold Nanoparticles by Pulsed Laser Ablation in NaOH solution Raheem Gaayied Kadhim Maryam falah noori Babylon university /colloge of the scince Abdulrahman Khalaf Ali School of Applied Sciences

More information

Supporting Information

Supporting Information Supporting Information Decorating Graphene Sheets with Gold Nanoparticles Ryan Muszynski, Brian Seeger and, Prashant V. Kamat* Radiation Laboratory, Departments of Chemistry & Biochemistry and Chemical

More information

Supporting information. An improved photo-induced fluorogenic alkene-tetrazole reaction for protein labeling

Supporting information. An improved photo-induced fluorogenic alkene-tetrazole reaction for protein labeling Supporting information An improved photo-induced fluorogenic alkene-tetrazole reaction for protein labeling X. Shang, 1 R. Lai, 1,3 X. Song, 1 H. Li, 1,3 W. Niu, 2 and J. Guo 1 * 1. Department of Chemistry,

More information

Magnetic Resonance Spectroscopy EPR and NMR

Magnetic Resonance Spectroscopy EPR and NMR Magnetic Resonance Spectroscopy EPR and NMR A brief review of the relevant bits of quantum mechanics 1. Electrons have spin, - rotation of the charge about its axis generates a magnetic field at each electron.

More information

Kinetics and Mechanism of Protection of URIDINE-5 - MONOPHOSPHATE from Sulphate Radical Anion by Caffeic acid Under Anoxic Conditions

Kinetics and Mechanism of Protection of URIDINE-5 - MONOPHOSPHATE from Sulphate Radical Anion by Caffeic acid Under Anoxic Conditions Research Article Kinetics and Mechanism of Protection of URIDINE-5 - MONOPHOSPHATE from Sulphate Radical Anion by Caffeic acid Under Anoxic Conditions Sudha Swaraga Midudhula 1 * and Adinarayana Mundra

More information

CHAPTER 3. FABRICATION TECHNOLOGIES OF CdSe/ZnS / Au NANOPARTICLES AND NANODEVICES. 3.1 THE SYNTHESIS OF Citrate-Capped Au NANOPARTICLES

CHAPTER 3. FABRICATION TECHNOLOGIES OF CdSe/ZnS / Au NANOPARTICLES AND NANODEVICES. 3.1 THE SYNTHESIS OF Citrate-Capped Au NANOPARTICLES CHAPTER 3 FABRICATION TECHNOLOGIES OF CdSe/ZnS / Au NANOPARTICLES AND NANODEVICES 3.1 THE SYNTHESIS OF Citrate-Capped Au NANOPARTICLES Au NPs with ~ 15 nm were prepared by citrate reduction of HAuCl 4

More information

International Journal of Pure and Applied Sciences and Technology

International Journal of Pure and Applied Sciences and Technology Int. J. Pure Appl. Sci. Technol., 9(1) (2012), pp. 1-8 International Journal of Pure and Applied Sciences and Technology ISSN 2229-6107 Available online at www.ijopaasat.in Research Paper Preparation,

More information

Supporting Information for. Suppression of OH Generation from the Photo-Fenton Reaction in the Presence of α-pinene Secondary Organic Aerosol Material

Supporting Information for. Suppression of OH Generation from the Photo-Fenton Reaction in the Presence of α-pinene Secondary Organic Aerosol Material 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Supporting Information for Suppression of OH Generation from the Photo-Fenton Reaction in the Presence of α-pinene Secondary Organic Aerosol Material Rachel F. Hems *,

More information

Application Note: Ocean Optics in the Teaching and Research Laboratories Susquehanna University Department of Chemistry July 2014

Application Note: Ocean Optics in the Teaching and Research Laboratories Susquehanna University Department of Chemistry July 2014 Application Note: Ocean Optics in the Teaching and Research Laboratories Susquehanna University Department of Chemistry July 2014 Authors: Dr. Swarna Basu (Associate Professor of Chemistry), Dr. Wade Johnson

More information

ENZYME KINETICS. Medical Biochemistry, Lecture 24

ENZYME KINETICS. Medical Biochemistry, Lecture 24 ENZYME KINETICS Medical Biochemistry, Lecture 24 Lecture 24, Outline Michaelis-Menten kinetics Interpretations and uses of the Michaelis- Menten equation Enzyme inhibitors: types and kinetics Enzyme Kinetics

More information

LET LET. < 10 MeV/u H He MeV/u C. Keywords: Water radiolysis, ion beam, track structure, G value, intratrack reaction, LET, scavenger

LET LET. < 10 MeV/u H He MeV/u C. Keywords: Water radiolysis, ion beam, track structure, G value, intratrack reaction, LET, scavenger Water radiolysis with heavy-ion beams of energies up to 28 GeV provided from HIMAC (Heavy Ion Medical Accelerator in Chiba), NIRS (National Institute of Radiological Sciences) has been investigated from

More information

TEM image of derivative 1 and fluorescence spectra of derivative 1 upon addition of

TEM image of derivative 1 and fluorescence spectra of derivative 1 upon addition of Electronic Supplementary Material (ESI) for Green Chemistry. This journal is The Royal Society of Chemistry 2016 Supramolecular ensemble of PBI derivative and Cu 2 O NPs: Potential photo catalysts for

More information

A colorimetric and fluorescent turn-on sensor for pyrophosphate. anion based on dicyanomethylene-4h-chromene framework

A colorimetric and fluorescent turn-on sensor for pyrophosphate. anion based on dicyanomethylene-4h-chromene framework Electronic Supplementary Information (ESI) A colorimetric and fluorescent turn-on sensor for pyrophosphate anion based on dicyanomethylene-4h-chromene framework Xiaomei Huang, Zhiqian Guo, Weihong Zhu*,

More information

Synthesis and Characterization of Hybrid Nanoparticles for Biomedical and Environmental Remediation Applications

Synthesis and Characterization of Hybrid Nanoparticles for Biomedical and Environmental Remediation Applications Synthesis and Characterization of Hybrid Nanoparticles for Biomedical and Environmental Remediation Applications Soubantika Palchoudhury Chemical Engineering University of Tennessee at Chattanooga 6 th

More information

Redox Chemistry of Neptunium in Solutions of Nitric Acid

Redox Chemistry of Neptunium in Solutions of Nitric Acid Redox Chemistry of Neptunium in Solutions of Nitric Acid Alena PAULENOVA Martin PRECEK, Kyle HARTIG, Nathan KNAPP Neptunium Redox Chemistry Coexistence of three oxidation states in acidic solutions NpO

More information

COMPUTER AIDED DRUG DESIGN (CADD) AND DEVELOPMENT METHODS

COMPUTER AIDED DRUG DESIGN (CADD) AND DEVELOPMENT METHODS COMPUTER AIDED DRUG DESIGN (CADD) AND DEVELOPMENT METHODS DRUG DEVELOPMENT Drug development is a challenging path Today, the causes of many diseases (rheumatoid arthritis, cancer, mental diseases, etc.)

More information

FLUORESCENCE STUDY ON TRYPTOPHAN POTASSIUM IODIDE INTERACTION

FLUORESCENCE STUDY ON TRYPTOPHAN POTASSIUM IODIDE INTERACTION PROCEEDINGS OF THE YEREVAN STATE UNIVERSITY C h e m i s t r y a n d B i o l o g y 08, 5(), p. 75 79 FLUORESCENCE STUDY ON TRYPTOPHAN POTASSIUM IODIDE INTERACTION C h emistr y H. A. SHILAJYAN, K. R. GRIGORYAN

More information

Application of IR Raman Spectroscopy

Application of IR Raman Spectroscopy Application of IR Raman Spectroscopy 3 IR regions Structure and Functional Group Absorption IR Reflection IR Photoacoustic IR IR Emission Micro 10-1 Mid-IR Mid-IR absorption Samples Placed in cell (salt)

More information

INTRODUCTION TO IONIZING RADIATION (Attix Chapter 1 p. 1-5)

INTRODUCTION TO IONIZING RADIATION (Attix Chapter 1 p. 1-5) INTRODUCTION TO IONIZING RADIATION (Attix Chapter 1 p. 1-5) Ionizing radiation: Particle or electromagnetic radiation that is capable of ionizing matter. IR interacts through different types of collision

More information

Supporting Information

Supporting Information Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2015 Supporting Information Materials The peptides were designed and synthesized as reported

More information

From Friday s material

From Friday s material 5.111 Lecture 35 35.1 Kinetics Topic: Catalysis Chapter 13 (Section 13.14-13.15) From Friday s material Le Chatelier's Principle - when a stress is applied to a system in equilibrium, the equilibrium tends

More information

Effect of gamma-irradiation on aqueous solutions of Apollofix dyes

Effect of gamma-irradiation on aqueous solutions of Apollofix dyes NUKLEONIKA 2007;52(3):109 113 ORIGINAL PAPER Effect of gamma-irradiation on aqueous solutions of Apollofix dyes Dilek Solpan, Murat Torun, Olgun Güven Abstract. Radiation processing has been considered

More information

A graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin

A graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin This journal is The Royal Society of Chemistry 11 Electronic Supplementary Information (ESI) A graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin Xiujuan Xu, a Jing Huang,

More information

Chapter XII: Chemical dosimeters

Chapter XII: Chemical dosimeters Chapter XII: Chemical dosimeters 1 Chemical dosimeters Introduction Fricke dosimeter Polymer gel dosimeter Alanine dosimeter Radiochromic film 2 Principle of chemical dosimeters In a chemical dosimeter

More information

A BODIPY-based fluorescent probe for the differential

A BODIPY-based fluorescent probe for the differential Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 15 Supporting information A BODIPY-based fluorescent probe for the differential recognition of Hg(II)

More information

SUPPORTING INFORMATION. Photoswitchable catalysis by a nanozyme mediated by a light-sensitive cofactor

SUPPORTING INFORMATION. Photoswitchable catalysis by a nanozyme mediated by a light-sensitive cofactor SUPPORTING INFORMATION Photoswitchable catalysis by a nanozyme mediated by a light-sensitive cofactor Simona Neri, Sergio García Martín, Cristian Pezzato, Leonard J. Prins* Department of Chemical Sciences,

More information

SUPPORTING INFORMATION

SUPPORTING INFORMATION SUPPRTING INRMATIN Light-Responsive Polymer Surfaces via Postpolymerization Modification of Grafted Polymer-Brush Structures Matthias Dübner,, Nicholas D. Spencer, Celestino Padeste Laboratory for Micro-

More information

Chapter 3. Electromagnetic Theory, Photons. and Light. Lecture 7

Chapter 3. Electromagnetic Theory, Photons. and Light. Lecture 7 Lecture 7 Chapter 3 Electromagnetic Theory, Photons. and Light Sources of light Emission of light by atoms The electromagnetic spectrum see supplementary material posted on the course website Electric

More information

Supporting Information. Self-assembled nanofibers from Leucine Derived Amphiphiles as Nanoreactors for Growth of ZnO Nanoparticles

Supporting Information. Self-assembled nanofibers from Leucine Derived Amphiphiles as Nanoreactors for Growth of ZnO Nanoparticles Supporting Information Self-assembled nanofibers from Leucine Derived Amphiphiles as Nanoreactors for Growth of ZnO Nanoparticles Karen T. Johnson, Theodore E. Gribb, Evan M. Smoak, and Ipsita A. Banerjee*

More information

Supplementary Information

Supplementary Information Supplementary Information 1. Experimental 1.1.1 Synthesis of hollow silica nanoparticles NPs. The precursor of sol-gel silica is fresh made 1.0 M Si(OH) 4 solution which was made by adding tetramethyl

More information

RESULTS AND DISCUSSION Characterization of pure CaO and Zr-TiO 2 /CaO nanocomposite

RESULTS AND DISCUSSION Characterization of pure CaO and Zr-TiO 2 /CaO nanocomposite RESULTS AND DISCUSSION 4.1. Characterization of pure CaO and Zr-TiO 2 /CaO nanocomposite 4.1.1. Scanning electron microscopy analysis (SEM) SEM images of prepared CaO are shown in Fig. 4.1 (a and b). CaO

More information

Growth of silver nanocrystals on graphene by simultaneous reduction of graphene oxide and silver ions with a rapid and efficient one-step approach

Growth of silver nanocrystals on graphene by simultaneous reduction of graphene oxide and silver ions with a rapid and efficient one-step approach Growth of silver nanocrystals on graphene by simultaneous reduction of graphene oxide and silver ions with a rapid and efficient one-step approach Xiu-Zhi Tang, a Zongwei Cao, b Hao-Bin Zhang, a Jing Liu

More information

Innovative. Technologies. Chemie des Klebens Chemistry of Adhesives. Dr. Jochen Stock, Laboratory Manager CRL Germany: Neuss, November 27 th, 2013

Innovative. Technologies. Chemie des Klebens Chemistry of Adhesives. Dr. Jochen Stock, Laboratory Manager CRL Germany: Neuss, November 27 th, 2013 Chemie des Klebens Chemistry of Adhesives Dr. Jochen Stock, Laboratory Manager CRL Germany: Neuss, November 27 th, 2013 Innovative Technologies 1 Overview Chemie des Klebens Chemistry of Adhesives Introduction

More information

Chapter 6 Photoluminescence Spectroscopy

Chapter 6 Photoluminescence Spectroscopy Chapter 6 Photoluminescence Spectroscopy Course Code: SSCP 4473 Course Name: Spectroscopy & Materials Analysis Sib Krishna Ghoshal (PhD) Advanced Optical Materials Research Group Physics Department, Faculty

More information

Supporting information

Supporting information Supporting information Fluorescent derivatives of AC-42 to probe bitopic orthosteric/allosteric binding mechanisms on muscarinic M1 receptors Sandrine B. Daval, Céline Valant, Dominique Bonnet, Esther

More information

Preparation of poly(vinyl alcohol) hydrogels with radiation grafted citric and succinic acid groups

Preparation of poly(vinyl alcohol) hydrogels with radiation grafted citric and succinic acid groups Radiation Physics and Chemistry 55 (1999) 667±671 www.elsevier.com/locate/radphyschem Preparation of poly(vinyl alcohol) hydrogels with radiation grafted citric and succinic acid groups Hatice BodugoÈ

More information

FACULTY OF PHARMACY. M. Pharmacy I Semester (Suppl.) Examination, November 2015 (Common To All) Subject: Pharmaceutical Analytical Techniques

FACULTY OF PHARMACY. M. Pharmacy I Semester (Suppl.) Examination, November 2015 (Common To All) Subject: Pharmaceutical Analytical Techniques M. Pharmacy I Semester (Suppl.) Examination, November 2015 (Common To All) Subject: Pharmaceutical Analytical Techniques Code No. 6001 / S 1 a) Describe the instrumentation and applications of UV-visible

More information

Supporting Information

Supporting Information Supporting Information Remarkable Photothermal Effect of Interband Excitation on Nanosecond Laser-induced Reshaping and Size Reduction of Pseudo-spherical Gold Nanoparticles in Aqueous Solution Daniel

More information

Supporting Information for. Chad A. Mirkin* Department of Chemistry and Institute for Nanotechnology, Northwestern University,

Supporting Information for. Chad A. Mirkin* Department of Chemistry and Institute for Nanotechnology, Northwestern University, S1 Supporting Information for Observation of a Quadrupole Plasmon Mode for a Colloidal Solution of Gold Nanoprisms Jill E. Millstone, Sungho Park, Kevin L. Shuford, Lidong Qin, George C. Schatz, and Chad

More information

Soluble and Crosslinked Hydrophilic Films Based on Compositions of Poly(acrylic acid) and Poly(2-hydroxyethyl vinyl ether) for Controlled Drug Release

Soluble and Crosslinked Hydrophilic Films Based on Compositions of Poly(acrylic acid) and Poly(2-hydroxyethyl vinyl ether) for Controlled Drug Release Soluble and Crosslinked Hydrophilic Films Based on Compositions of Poly(acrylic acid) and Poly(2-hydroxyethyl vinyl ether) for Controlled Drug Release Z. S. Nurkeeva, 1 G. A. Mun, 1 V. V. Khutoryanskiy,

More information

Defense Technical Information Center Compilation Part Notice

Defense Technical Information Center Compilation Part Notice UNCLASSIFIED Defense Technical Information Center Compilation Part Notice ADP12139 TITLE: Nano-Sized [6]Fullerene-Cyclodextrin Molecules DISTRIBUTION: Approved for public release, distribution unlimited

More information

Degree of labeling (DOL)

Degree of labeling (DOL) Degree of labeling (DOL) A written explanation for the determination of the degree of labeling when using NuLink reagents. The DOL is the average number of reagent molecules that have been covalently attached

More information

Spectroscopy. Page 1 of 8 L.Pillay (2012)

Spectroscopy. Page 1 of 8 L.Pillay (2012) Spectroscopy Electromagnetic radiation is widely used in analytical chemistry. The identification and quantification of samples using electromagnetic radiation (light) is called spectroscopy. Light has

More information

arxiv: v1 [physics.bio-ph] 11 Sep 2015

arxiv: v1 [physics.bio-ph] 11 Sep 2015 arxiv:1509.04625v1 [physics.bio-ph] 11 Sep 2015 IR-Laser Welding and Ablation of Biotissue Stained with Metal Nanoparticles A. A. Lalayan, S. S. Israelyan Centre of Strong Fields Physics, Yerevan State

More information

Treatment of effluents from petroleum production by electron beam irradiation

Treatment of effluents from petroleum production by electron beam irradiation Radiation Physics and Chemistry 71 (2004) 443 447 Treatment of effluents from petroleum production by electron beam irradiation Celina Lopes Duarte a, *, Lucia Limoeiro Geraldo b, Oswaldo de Aquino P.

More information

Hydrogel Biomaterials: Structure and thermodynamics

Hydrogel Biomaterials: Structure and thermodynamics Hydrogel Biomaterials: Structure and thermodynamics Last Day: programmed/regulated/multifactor controlled release for drug delivery and tissue eng ineering Announcements: Today: Reading: Finish discussion

More information

Available online at ScienceDirect. Procedia Materials Science 11 (2015 )

Available online at   ScienceDirect. Procedia Materials Science 11 (2015 ) Available online at www.sciencedirect.com ScienceDirect Procedia Materials Science 11 (2015 ) 282 286 5th International Biennial Conference on Ultrafine Grained and Nanostructured Materials, UFGNSM15 Prepartion

More information

Supporting Information

Supporting Information Supporting Information Figure S1. 2D ( 1 H- 1 H) COSY90 NMR (300 MHz, 3:1 TFA:TFA-d) spectrum of oligo(l-glu-co- 25%L-Cys) synthesized using from 7:3 L-Glu-(Et) 2 :L-Cys-Et, 0.5 M total substrate concentration,

More information

Supporting Information. for. Angew. Chem. Int. Ed. Z Wiley-VCH 2004

Supporting Information. for. Angew. Chem. Int. Ed. Z Wiley-VCH 2004 Supporting Information for Angew. Chem. Int. Ed. Z 54131 Wiley-VCH 2004 69451 Weinheim, Germany Angew. Chem. Int. Ed., Z. Y. Tian Z54131 Supporting Info Page 1 Supplementary Materials: Material A providing

More information

Effects of Temperature and Concentration on the Rate of Photo-bleaching of Erythrosine in Water

Effects of Temperature and Concentration on the Rate of Photo-bleaching of Erythrosine in Water Supporting Information for: Effects of Temperature and Concentration on the Rate of Photo-bleaching of Erythrosine in Water Joshua K. G. Karlsson, Owen J. Woodford, Roza Al-Aqar and Anthony Harriman* Molecular

More information

Ratiometric Fluorescence Imaging of Cellular Glutathione

Ratiometric Fluorescence Imaging of Cellular Glutathione Supporting Information for: Ratiometric Fluorescence Imaging of Cellular Glutathione Gun-Joong Kim, Kiwon Lee, Hyockman Kwon, and Hae-Jo Kim, * Department of Chemistry, and Department of Bioscience and

More information

Supporting Information

Supporting Information Supporting Information Activating Room Temperature Long Afterglow of Carbon Dots via Covalent Fixation Kai Jiang,, Yuhui Wang, Congzhong Cai, and Hengwei Lin*, Key Laboratory of Graphene Technologies and

More information

Ciência (Bio)nanosystems based on quantum dots, plasmonic or magnetic nanoparticles

Ciência (Bio)nanosystems based on quantum dots, plasmonic or magnetic nanoparticles Ciência 216 (Bio)nanosystems based on quantum dots, plasmonic or magnetic nanoparticles Paulo J. G. Coutinho Departamento/Centro de Física Escola de Ciências Universidade do Minho 4 Julho 216 OVERVIEW

More information

Photocatalysis: semiconductor physics

Photocatalysis: semiconductor physics Photocatalysis: semiconductor physics Carlos J. Tavares Center of Physics, University of Minho, Portugal ctavares@fisica.uminho.pt www.fisica.uminho.pt 1 Guimarães Where do I come from? 3 Guimarães 4 Introduction>>

More information

Design of Pyrrolic-N-rich Carbon Dots with. Absorption in the First Near Infrared Window for. Photothermal Therapy

Design of Pyrrolic-N-rich Carbon Dots with. Absorption in the First Near Infrared Window for. Photothermal Therapy Supporting Information Design of Pyrrolic-N-rich Carbon Dots with Absorption in the First Near Infrared Window for Photothermal Therapy Fitri A. Permatasari, Hiromitsu Fukazawa, Takashi Ogi,*, Ferry Iskandar,,

More information

UV-POLYMERIZATION IN THE PRESENCE OF NANOFILLERS CHAP Introduction

UV-POLYMERIZATION IN THE PRESENCE OF NANOFILLERS CHAP Introduction UV-POLYMERIZATION IN THE PRESENCE OF NANOFILLERS 3.1 Introduction The first problem in preparing UV-cured composites is the filler transparency towards UV-light. Otherwise it will be a competition of the

More information

Synthetic polypetides for materials science and biosensing. Professor Bo Liedberg, Molecular Physics, Linköping University, Sweden

Synthetic polypetides for materials science and biosensing. Professor Bo Liedberg, Molecular Physics, Linköping University, Sweden bolie@ifm.liu.se www.ifm.liu.se/applphys/sensor Synthetic polypetides for materials science and biosensing Professor Bo Liedberg, Molecular Physics, Linköping University, Sweden 436 th Heraeus Seminar,

More information

Energy transfer process in the reaction system NH 2 OH-NaOH-Cu(II)-Eu(III)/thenoyltrifluoroacetone

Energy transfer process in the reaction system NH 2 OH-NaOH-Cu(II)-Eu(III)/thenoyltrifluoroacetone Vol. 07 INTERNATIONAL JOURNAL OF PHOTOENERGY 2005 Energy transfer process in the reaction system NH 2 OH-NaOH-Cu(II)-Eu(III)/thenoyltrifluoroacetone Stefan Lis and Małgorzata Kaczmarek Department of Rare

More information

Measuring nanoparticle properties: experiences from NPL Caterina Minelli

Measuring nanoparticle properties: experiences from NPL Caterina Minelli Measuring nanoparticle properties: experiences from NPL Caterina Minelli Measurement of Particles Types of materials: Metal Examples: Silver Gold Palladium Platinum Semiconductor Examples: Quantum Dots

More information

Self Assembly of Cyanine Dye on Clay Nanoparticles

Self Assembly of Cyanine Dye on Clay Nanoparticles Self Assembly of Cyanine Dye on Clay Nanoparticles Andrew Dixon, Clint Duncan, and Hussein Samha* Department of Physical Science Southern Utah University Cedar City, Utah 8472 USA Received: January 1,

More information