CHM 223 Organic Chemistry I Prof. Chad Landrie. Lecture 10: September 20, 2018 Ch. 12: Spectroscopy mass spectrometry infrared spectroscopy
|
|
- Marilyn Dalton
- 5 years ago
- Views:
Transcription
1 M 223 Organic hemistry I Prof. had Landrie Lecture 10: September 20, 2018 h. 12: Spectroscopy mass spectrometry infrared spectroscopy
2 i>licker Question onsider a solution that contains 65g R enantiomer and 35g S enantiomer. If the observed specific optical rotation for the mixture is 74.0º, what is the specific optical rotation of the pure R isomer? A. 247 B D E Slide 2
3 Spectroscopy vs. Spectrometry Spectroscopy = study of the interaction of electromagnetic radiation with matter; typically involves the absorption of electromagnetic radiation Spectrometry = evaluation of molecular identity and/or properties that does not involve interaction with electromagnetic radiation Slide 3 3
4 Spectroscopic and Spectrometric Methods Method Mass Spectrometry (MS) Infrared (IR) Spectroscopy Nuclear Magnetic Resonance (NMR) Spectroscopy Ultraviolet-Visible (UV-vis )Spectroscopy Measurement/Application Used to determine the molecular mass and the molecular formula. reates radical cations and measures their movement through a magnetic field. Used to determine the functional groups in a substance Measures the stretching and bending frequencies of covalent bonds that contain a dipole moment. Used to determine the connectivity (, framework) of molecule Measures the energy associated with spin states of nuclei in the presence of a magnetic field Used to determine the nature of conjugated p-systems (e.g. adjacent double bonds, aromatic rings) Measures the energy associated with promotion of an electron in a ground state to an exited state Slide 4 4
5 Spectroscopic and Spectrometric Methods Method Mass Spectrometry (MS) Measurement/Application Used to determine the molecular mass and the molecular formula. reates radical cations and measures their movement through a magnetic field. eroin Slide 5 5
6 Spectroscopic and Spectrometric Methods Method Infrared (IR) Spectroscopy Measurement/Application Used to determine the functional groups in a substance Measures the stretching and bending frequencies of covalent bonds that contain a dipole moment. eroin Slide 6 6
7 Spectroscopic and Spectrometric Methods Method Nuclear Magnetic Resonance (NMR) Spectroscopy Measurement/Application Used to determine the connectivity (, framework) of molecule Measures the energy associated with spin states of nuclei in the presence of a magnetic field Slide 7 7
8 Spectroscopic and Spectrometric Methods Method Ultraviolet-Visible (UV-vis )Spectroscopy Measurement/Application Used to determine the nature of conjugated p-systems (e.g. adjacent double bonds, aromatic rings) Measures the energy associated with promotion of an electron in a ground state to an exited state tetraphenylcyclopentadienone O Slide 8 8
9 M 223 Organic hemistry I Prof. had Landrie Mass Spectrometry Sections
10 Mass Spectrometry Taxol Primary Applications: 1. Determine molecular mass. 2. Establish fragmentation patterns (mass of molecular fragments formed when covalent bonds are broken), which can be indexed in a database. 3. Determine presence of some heteroatoms (e.g., l, Br). 4. Determine the exact mass of molecules. Slide 10
11 Mass Spectrometer Schematic creates charged molecules called radical cations radical cations are accelerated by negatively charged plates magnetic fields exert forces on moving charges Slide 11
12 Formation of Radical ations radical = unpaired electron organic molecules are bombarded with 70-eV electrons causes organic molecule to lose one electron from a covalent bond or electron lone-pair organic molecule is then charged the mass of charged species is determined by a mass spectrometer Slide 12
13 Lorentz Force Like electric currents, moving charges create magnetic fields. These magnetic fields experience Lorentz forces when passed perpendicularly past an external magnetic field. The magnitude depends on the size, mass and velocity of the charge. Slide 13
14 Lorentz Force Slide 14
15 Mass Spectrometer Schematic creates charged molecules called radical cations radical cations are accelerated by negatively charged plates magnetic fields exert forces on moving charges Slide 15
16 Molecular Ion Peaks : 6 x 12 = 72 : 6 x 1 = 6 Total = 78 molecular ion peak = highest m/z (mass/charge) peak since charge (z) is usually 1, molecular ion peak = molecular mass molecular ion peak does not have to have relative intensity of 100% most intense peak = base peak relative intensity = height of peak base peak Slide 16
17 Fragmentation heterolysis radical cation fragments (heterolysis) to give a neutral radical species and a cation fragment contains less mass than parent ion relative intensity of the fragment depends on its concentration (likelihood of occurring) more stable cations are more likely; give more intense peaks Slide 17
18 Fragmentation ommon Fragmentation Pattern for Alkanes Slide 18
19 Fragmentation ommon Fragmentation Pattern for Alkyl Benzenes benzylic carbon benzylic carbocation benzylic carbocation stabilized by resonance = common fragment in MS Slide 19
20 Fragmentation Slide 20
21 Fragmentation Since fragmentation patterns should be the same for identical molecules, they can be saved in a database and matched to unknowns later. SI anyone? Slide 21
22 Isotopic lusters: arbon and ydrogen 14 M + 1 M + 1 Slide 22
23 Isotopic lusters: arbon and ydrogen Probability of M+1 6 x 1.1% = 6.6% of 13 6 x 0.015% = 0.1% of 2 M + 1 M + 1 Total Probablility = 6.7% Natural Abundance of Isotopes Isotope Abundance % % 2 (D) 0.015% If there are n carbon atoms in a molecular ion, then the ratio of M+ to [M+1]+ is 100:(1.10 x n) % Slide 23
24 Isotopic lusters: hlorine & Bromine Natural Abundance of Isotopes Isotope Abundance 35 l 75.77% 37 l 24.23% 79 Br 50.69% hlorine: M + :[M+2] + ~ 3:1 Bromine: M + :[M+2] + ~ 1:1 81 Br 49.31% Slide 24
25 Isotopic lusters: hlorine Duclofenac: analgesic behaves similarly to aspirin more soluble/bioavailable l l N M + = O O (295, 40) (297, 26.6) = = 7.0 Natural Abundance of Isotopes Isotope Abundance 35 l 75.77% 37 l 24.23% Predicting Ratio of Relative Intensity for 2 l Atoms Molecular Ion hlorine ombination Math % Ratio [M] + 35 l x 35 l x (299, 3.8) [M+2] + 37 l x 35 l x l x 37 l x [M+4] + 37 l x 37 l x Slide 25
26 i>licker Question Duclofenac is an analgesic that behaves similarly to aspirin except that it is more soluble and thus bioavailable. What atom is most likely represented by X based on the mass spectrum? Natural Abundance of Isotopes Isotope Abundance 35 l 75.77% 37 l 24.23% 79 Br 50.69% X X N O A. l B. Br 81 Br 49.31% % % 2 (D) 0.015% % O. D. Slide 26
27 i>licker Question Which peak in the mass spectrum represents the molecular ion peak and gives the molecular weight? A. 295 l 214 B. 278 l N O O D. 214 E. 179 Slide 27
28 i>licker Question Which peak in the mass spectrum represents the base peak? A. 295 l 214 B. 278 l N O O D. 214 E. 179 Slide 28
29 i>licker Question Which peak in the mass spectrum represents the molecular ion formed by loss of a hydroxyl group (-O) from this molecule? A. 295 l l N O B O D. 214 E. 179 Slide 29
30 i>licker Question Which peak in the mass spectrum represents the molecular ion formed by loss of a carboxylic acid group (-O2) and a chlorine atom (-l) from this molecule? A. 295 l 215 B. 278 l N O O D. 214 E. 179 Slide 30
31 M 223 Organic hemistry I Prof. had Landrie Infrared Spectroscopy Sections
32 Electromagnetic Spectrum shorter wavelength (λ) higher frequency (ν) higher energy (E) longer wavelength (λ) lower frequency (ν) lower energy (E) Electromagnetic Radiation propagated at the speed of light (3 x10 8 m/s) has properties of particles and waves energy is directly proportional to frequency energy is indirectly proportional to wavelength E = hν c = νλ Slide 32
33 Review: Principles of Infrared Spectroscopy IR: Measures the vibrational energy associated with stretching or bending bonds that contain a dipole moment (µ). Stretching δ + δ + δ + δ δ δ δ Bending δ δ δ + δ + δ + Slide 33
34 Stretching & Bending Vibrations Slide 34
35 Dipole Moment more electronegative atom covalent 2 electron bond dipole arrow less electronegative atom δ (partially negatively charged) δ (partially positively charged) In order to measure the stretching or bending frequency of a covalent bond, it must have a dipole moment (μ). Slide 35
36 ooke s Law: Bonds are Like Springs Vibrational Energy Depends both on bond strength (spring force constant) and the mass of atoms (objects) attached Trends: ~ ν = vibrational frequency in wavenumbers (cm -1 ) k = force constant; strength of bond (spring) m* = reduced mass ma = mass of 1 atom of a bond strength = frequency mass = frequency Slide 36
37 Spring Analogy smaller mass = higher frequency = higher energy stronger spring (bond) = higher frequency = higher energy Slide 37
38 i>licker Question Which covalent bond, highlighted in bold (red) in the molecules below, would not be expected to exhibit an IR stretching band? O O 3 N 3 N A B D E 3 3 Slide 38
39 Wavenumber (ῡ) and Infrared Scale ῡ (cm -1 ) = 1 λ (cm) higher wavenumber (ῡ) = higher frequency (υ) = lower wavelength (λ) = higher energy (E) N- O- (sp)- (sp2)- (sp3)- O 2 (2380) N O N lower wavenumber (ῡ) = lower frequency (υ) = longer wavelength (λ) = lower energy (E) fingerprint region O wavenumber (cm -1 ) wavenumber = reciprocal of the wavelength measured in centimeters (cm); directly proportional to frequency Slide 39
40 General Need-to-Know IR Frequencies Slide 41
41 Infrared Spectrum % Transmission Transmittance: amount of light that passes through sample; not absorbed by molecular vibrations Frequency: typically measured in wavenumbers; higher wavenumber = higher frequency = higher energy vibration Bands: frequency of vibration absorbed by molecules; can be broad or narrow; number of bands does not equal number of bonds Wavenumbers Slide 42
42 Example: Alkanes Wavenumbers = sp 3 - bond stretching motion; general absorb around cm -1 1 = - rocking motion when atom is part of a methyl group (- 3); cm -1 3 = scissor motion of -3 hydrogen atoms; cm cm -1 = fingerprint region for organic molecules; typically complex and unhelpful hexane 3 2 Slide 43
43 Example: Alkenes : notice sp2 - (~3100 cm -1 ) at higher frequency than sp3 - (~2950 cm -1 ) 1-hexene Wavenumbers more s-character = stronger bond = higher frequency 4: also, = bond at higher frequency than - bond; ~1600 cm -1 Slide 44
44 Example: Alkynes hexyne 7: notice sp - (~3300 cm -1 ) at higher frequency than sp2 - (~3100 cm -1 ), which was higher than sp3 - (~2950 cm -1 ) 6: stretch is very weak because carbons have almost identical electronegativities = small dipole moment Slide 45
45 Example: Alcohols 5 9 O 4 prop-2-en-1-ol (allyl alcohol) 9: hydroxyl groups (-O) exhibit strong broad bands; ~3300 cm -1 broad peak is a result of hydrogen bonding; width depends on solution concentration lower concentration = less hydrogen bonding = more narrow -O band Slide 46
46 Example: Nitriles O N hydroxy-propionitrile 8: nitriles ~2200 cm nitriles ( N) absorb a greater magnitude of energy than alkynes ( ) because they have a larger dipole moment larger dipole moment = more intense peak Wavenumbers size of the dipole does NOT affect frequency of vibration Slide 47
47 Example: Ester, Amine, Benzene 100 O N O Wavenumbers amino-benzoic acid butyl ester 10: strong carbonyl (=O) band ~1700 cm -1 11: amines; secondary amines (- N) give one band; primary amines (-N2) gives two bands 4: several alkene bands ~1600 cm -1 for benzene ring = double bonds Slide 48
48 Example: arboxylic Acid O 10 O 9 60 cyclohex-2-enecarboxylic acid 40 10: strong carbonyl (=O) band ~1700 cm : hydroxyl band (-O) can be less intense and sharper in carboxylic acids Wavenumbers : weak alkene band (=) since small dipole moment Slide 49
49 Example: Aldehyde 100 O hept-2-enal 12: usually two bands for - of aldehydes; may overlap with sp3 - bands (Fermi doublet) Wavenumbers Slide 50
50 M 223 Organic hemistry I Prof. had Landrie Next Lecture... hapter 12
Lecture 13 Organic Chemistry 1
EM 232 rganic hemistry I at hicago Lecture 13 rganic hemistry 1 Professor Duncan Wardrop February 23, 2010 1 EM 232 rganic hemistry I at hicago Spectroscopy & Spectrometry hapter 13 2 EM 232 rganic hemistry
More informationLecture 14 Organic Chemistry 1
CHEM 232 Organic Chemistry I at Chicago Lecture 14 Organic Chemistry 1 Professor Duncan Wardrop February 25, 2010 1 CHEM 232 Organic Chemistry I at Chicago Mass Spectrometry Sections: 13.24-13.25 2 Spectroscopy
More informationInfrared Spectroscopy
Infrared Spectroscopy Introduction Spectroscopy is an analytical technique which helps determine structure. It destroys little or no sample. The amount of light absorbed by the sample is measured as wavelength
More informationChapter 12 Mass Spectrometry and Infrared Spectroscopy
Organic Chemistry, 6 th Edition L. G. Wade, Jr. Chapter 12 Mass Spectrometry and Infrared Spectroscopy Jo Blackburn Richland College, Dallas, TX Dallas County Community College District 2006, Prentice
More informationLecture 11. IR Theory. Next Class: Lecture Problem 4 due Thin-Layer Chromatography
Lecture 11 IR Theory Next Class: Lecture Problem 4 due Thin-Layer Chromatography This Week In Lab: Ch 6: Procedures 2 & 3 Procedure 4 (outside of lab) Next Week in Lab: Ch 7: PreLab Due Quiz 4 Ch 5 Final
More informationRadiant energy is proportional to its frequency (cycles/s = Hz) as a wave (Amplitude is its height) Different types are classified by frequency or
CHEM 241 UNIT 5: PART B INFRA-RED RED SPECTROSCOPY 1 Spectroscopy of the Electromagnetic Spectrum Radiant energy is proportional to its frequency (cycles/s = Hz) as a wave (Amplitude is its height) Different
More informationInfrared Spectroscopy: Identification of Unknown Substances
Infrared Spectroscopy: Identification of Unknown Substances Suppose a white powder is one of the four following molecules. How can they be differentiated? H N N H H H H Na H H H H H A technique that is
More informationWelcome to Organic Chemistry II
Welcome to Organic Chemistry II Erika Bryant, Ph.D. erika.bryant@hccs.edu Class Syllabus 3 CHAPTER 12: STRUCTURE DETERMINATION 4 What is this solution Soda Tea Coffee??? 5 What is this solution Soda Tea
More informationSymmetric Stretch: allows molecule to move through space
BACKGROUND INFORMATION Infrared Spectroscopy Before introducing the subject of IR spectroscopy, we must first review some aspects of the electromagnetic spectrum. The electromagnetic spectrum is composed
More information12. Structure Determination: Mass Spectrometry and Infrared Spectroscopy
12. Structure Determination: Mass Spectrometry and Infrared Spectroscopy Determining the Structure of an Organic Compound The analysis of the outcome of a reaction requires that we know the full structure
More informationSPECTROSCOPY MEASURES THE INTERACTION BETWEEN LIGHT AND MATTER
SPECTROSCOPY MEASURES THE INTERACTION BETWEEN LIGHT AND MATTER c = c: speed of light 3.00 x 10 8 m/s (lamda): wavelength (m) (nu): frequency (Hz) Increasing E (J) Increasing (Hz) E = h h - Planck s constant
More informationIntroduction. The analysis of the outcome of a reaction requires that we know the full structure of the products as well as the reactants
Introduction The analysis of the outcome of a reaction requires that we know the full structure of the products as well as the reactants Spectroscopy and the Electromagnetic Spectrum Unlike mass spectrometry,
More information(2) Read each statement carefully and pick the one that is incorrect in its information.
Organic Chemistry - Problem Drill 17: IR and Mass Spectra No. 1 of 10 1. Which statement about infrared spectroscopy is incorrect? (A) IR spectroscopy is a method of structure determination based on the
More informationLearning Guide for Chapter 3 - Infrared Spectroscopy
Learning Guide for hapter 3 - Infrared Spectroscopy I. Introduction to spectroscopy - p 1 II. Molecular vibrations - p 3 III. Identifying functional groups - p 6 IV. Interpreting an IR spectrum - p 12
More informationOAT Organic Chemistry - Problem Drill 19: NMR Spectroscopy and Mass Spectrometry
OAT Organic Chemistry - Problem Drill 19: NMR Spectroscopy and Mass Spectrometry Question No. 1 of 10 Question 1. Which statement concerning NMR spectroscopy is incorrect? Question #01 (A) Only nuclei
More information13.24: Mass Spectrometry: molecular weight of the sample
hapter 13: Spectroscopy Methods of structure determination Nuclear Magnetic Resonances (NMR) Spectroscopy (Sections 13.3-13.19) Infrared (IR) Spectroscopy (Sections 13.20-13.22) Ultraviolet-visible (UV-Vis)
More informationE35 SPECTROSCOPIC TECHNIQUES IN ORGANIC CHEMISTRY
E35 SPECTRSCPIC TECNIQUES IN RGANIC CEMISTRY Introductory Comments. These notes are designed to introduce you to the basic spectroscopic techniques which are used for the determination of the structure
More informationInfrared Spectroscopy An Instrumental Method for Detecting Functional Groups
Infrared Spectroscopy An Instrumental Method for Detecting Functional Groups 1 The Electromagnetic Spectrum Infrared Spectroscopy I. Physics Review Frequency, υ (nu), is the number of wave cycles that
More informationORGANIC - BRUICE 8E CH MASS SPECT AND INFRARED SPECTROSCOPY
!! www.clutchprep.com CONCEPT: PURPOSE OF ANALYTICAL TECHNIQUES Classical Methods (Wet Chemistry): Chemists needed to run dozens of chemical reactions to determine the type of molecules in a compound.
More informationCH 3. mirror plane. CH c d
CAPTER 20 Practice Exercises 20.1 The index of hydrogen deficiency is two. The structural possibilities include two double bonds, a double do 20.3 (a) As this is an alkane, it contains only C and and has
More informationChapter 14 Spectroscopy
hapter 14 Spectroscopy There are four major analytical techniques used for identifying the structure of organic molecules 1. Nuclear Magnetic Resonance or NMR is the single most important technique for
More informationChapter 13 Spectroscopy
hapter 13 Spectroscopy Infrared spectroscopy Ultraviolet-Visible spectroscopy Nuclear magnetic resonance spectroscopy Mass Spectrometry 13.1 Principles of Molecular Spectroscopy: Electromagnetic Radiation
More informationUnit 2 Organic Chemistry. 2.3 Structural Analysis Part 2:
CFE ADVANCED HIGHER Unit 2 Organic Chemistry 2.3 Structural Analysis Part 2: Mass Spectroscopy Infra-red Spectroscopy NMR Proton Spectroscopy Answers to Questions in Notes Learning Outcomes Exam Questions
More informationCHEM 3.2 (AS91388) 3 credits. Demonstrate understanding of spectroscopic data in chemistry
CHEM 3.2 (AS91388) 3 credits Demonstrate understanding of spectroscopic data in chemistry Spectroscopic data is limited to mass, infrared (IR) and 13 C nuclear magnetic resonance (NMR) spectroscopy. Organic
More informationMOLECULAR REPRESENTATIONS AND INFRARED SPECTROSCOPY
MOLEULAR REPRESENTATIONS AND INFRARED SPETROSOPY A STUDENT SOULD BE ABLE TO: 1. Given a Lewis (dash or dot), condensed, bond-line, or wedge formula of a compound draw the other representations. 2. Give
More informationLearning Guide for Chapter 3 - Infrared Spectroscopy
Learning Guide for hapter 3 - Infrared Spectroscopy I. Introduction to spectroscopy - p 1 II. Molecular vibrations - p 3 III. Identifying functional groups - p 6 IV. Interpreting an IR spectrum - p 12
More informationOrganic Chemistry: CHEM2322
Etiquette & Course Overview Organic Chemistry: Structure Determination MS & IR Dr. Christopher J. O Brien 203 CRB, cobrien@uta.edu Classroom etiquette Arrive on time if you are late you must quietly take
More informationUnit 3 Organic Chemistry. 3.3 Structural Analysis Part 2:
Unit 3 Organic Chemistry 3.3 Structural Analysis Part 2: Mass Spectroscopy Infra-red Spectroscopy NMR Proton Spectroscopy Answers to Questions in Notes Learning Outcomes Exam Questions & Answers MODIFIED
More informationMass Spectrometry. 2000, Paul R. Young University of Illinois at Chicago, All Rights Reserved
Mass Spectrometry 2000, Paul R. Young University of Illinois at Chicago, All Rights Reserved Mass Spectrometry When a molecule is bombarded with high-energy electrons, one of the process that can occur
More informationEXPT. 7 CHARACTERISATION OF FUNCTIONAL GROUPS USING IR SPECTROSCOPY
EXPT. 7 CHARACTERISATION OF FUNCTIONAL GROUPS USING IR SPECTROSCOPY Structure 7.1 Introduction Objectives 7.2 Principle 7.3 Requirements 7.4 Strategy for the Interpretation of IR Spectra 7.5 Practice Problems
More informationChapter 13. R.F.----µ-wave----I.R. (Heat)------Visible------U.V X-Ray------γ-Ray SPECTROSCOPY. Definition: Types to Be Covered:
hamras Glendale ommunity ollege rganic hemistry 105 Exam 4 Materials hapter 13 SPETRSPY Definition: Types to Be overed: A) Infrared Spectroscopy (IR) B) Nuclear Magnetic Resonance Spectroscopy (NMR) )
More information7a. Structure Elucidation: IR and 13 C-NMR Spectroscopies (text , , 12.10)
2009, Department of Chemistry, The University of Western Ontario 7a.1 7a. Structure Elucidation: IR and 13 C-NMR Spectroscopies (text 11.1 11.5, 12.1 12.5, 12.10) A. Electromagnetic Radiation Energy is
More informationInfrared Spectroscopy
Infrared Spectroscopy IR Spectroscopy Used to identify organic compounds IR spectroscopy provides a 100% identification if the spectrum is matched. If not, IR at least provides information about the types
More informationORGANIC - CLUTCH CH ANALYTICAL TECHNIQUES: IR, NMR, MASS SPECT
!! www.clutchprep.com CONCEPT: PURPOSE OF ANALYTICAL TECHNIQUES Classical Methods (Wet Chemistry): Chemists needed to run dozens of chemical reactions to determine the type of molecules in a compound.
More informationCHEM 241 UNIT 5: PART A DETERMINATION OF ORGANIC STRUCTURES BY SPECTROSCOPIC METHODS [MASS SPECTROMETRY]
CHEM 241 UNIT 5: PART A DETERMINATION OF ORGANIC STRUCTURES BY SPECTROSCOPIC METHODS [MASS SPECTROMETRY] 1 Introduction Outline Mass spectrometry (MS) 2 INTRODUCTION The analysis of the outcome of a reaction
More informationORGANIC - CLUTCH CH ANALYTICAL TECHNIQUES: IR, NMR, MASS SPECT
!! www.clutchprep.com CONCEPT: PURPOSE OF ANALYTICAL TECHNIQUES Classical Methods (Wet Chemistry): Chemists needed to run dozens of chemical reactions to determine the type of molecules in a compound.
More informationi e l d f Energy (E) = Direction visible ultraviolet X-ray gamma infrared
rganic Structure Determination Analytical hemistry Instrument-based methods for determination of structure of organic molecules 1) Infrared Spectroscopy - yields functional groups 2) M Spectroscopy - very
More informationAdvanced Pharmaceutical Analysis
Lecture 2 Advanced Pharmaceutical Analysis IR spectroscopy Dr. Baraa Ramzi Infrared Spectroscopy It is a powerful tool for identifying pure organic and inorganic compounds. Every molecular compound has
More informationStructure Determination. How to determine what compound that you have? One way to determine compound is to get an elemental analysis
Structure Determination How to determine what compound that you have? ne way to determine compound is to get an elemental analysis -basically burn the compound to determine %C, %H, %, etc. from these percentages
More informationQuímica Orgânica I. Ciências Farmacêuticas Bioquímica Química. IR spectroscopy AFB QO I 2007/08 1 AFB QO I 2007/08 2
Química Orgânica I Ciências Farmacêuticas Bioquímica Química AFB QO I 2007/08 1 IR spectroscopy AFB QO I 2007/08 2 1 Adaptado de: Organic Chemistry, 6th Edition; L. G. Wade, Jr. Organic Chemistry, William
More informationCalculate a rate given a species concentration change.
Kinetics Define a rate for a given process. Change in concentration of a reagent with time. A rate is always positive, and is usually referred to with only magnitude (i.e. no sign) Reaction rates can be
More informationObjective 4. Determine (characterize) the structure of a compound using IR, NMR, MS.
Objective 4. Determine (characterize) the structure of a compound using IR, NMR, MS. Skills: Draw structure IR: match bond type to IR peak NMR: ID number of non-equivalent H s, relate peak splitting to
More informationMore information can be found in Chapter 12 in your textbook for CHEM 3750/ 3770 and on pages in your laboratory manual.
CHEM 3780 rganic Chemistry II Infrared Spectroscopy and Mass Spectrometry Review More information can be found in Chapter 12 in your textbook for CHEM 3750/ 3770 and on pages 13-28 in your laboratory manual.
More informationMASS SPECTROSCOPY (MS)
MASS SPECTOSCOPY (MS) Castor seeds icin (toxic protein) INTODUCTION Does not involve absorption of electromagnetic radiation. It is a spectroscopic technique, by virtue of its use in structure elucidation.
More informationStructural Determination Of Compounds
EXPERIMENT 10 Mass Spectroscopy Structural Determination Of Compounds. Introduction - In mass spectrometry, a substance is bombarded with an electron beam having sufficient energy to fragment the molecule.
More informationInfrared Spectroscopy used to analyze the presence of functional groups (bond types) in organic molecules How IR spectroscopy works:
Infrared Spectroscopy used to analyze the presence of functional groups (bond types) in organic molecules It is the study of the interaction of infrared energy with organic molecules; the process analyzes
More informationChapter 9. Nuclear Magnetic Resonance and Mass Spectrometry. 1. Introduction. 2. Nuclear Magnetic Resonance (NMR) Spectroscopy
hapter 9 Nuclear Magnetic Resonance and Mass Spectrometry reated by Professor William Tam & Dr. Phillis hang 1. Introduction Spectroscopy the study of the interaction of light with matter Spectroscopy
More informationORGANIC - EGE 5E CH UV AND INFRARED MASS SPECTROMETRY
!! www.clutchprep.com CONCEPT: IR SPECTROSCOPY- FREQUENCIES There are specific absorption frequencies in the functional group region that we should be familiar with EXAMPLE: What are the major IR absorptions
More informationIncreasing energy. ( 10 4 cm -1 ) ( 10 2 cm -1 )
The branch of science which deals with the interaction of electromagnetic radiation with matter is called spectroscopy The energy absorbed or emitted in each transition corresponds to a definite frequency
More informationInfrared Spectroscopy
Infrared Spectroscopy (Chapter 12) 1 This reaction from Ochem 1 How do we know if it worked? The reactant is cyclohexene; the product is cyclohexanol. How can we tell the difference? Infrared Spectroscopy
More informationORGANIC - BROWN 8E CH INFRARED SPECTROSCOPY.
!! www.clutchprep.com CONCEPT: PURPOSE OF ANALYTICAL TECHNIQUES Classical Methods (Wet Chemistry): Chemists needed to run dozens of chemical reactions to determine the type of molecules in a compound.
More informationIdentification of functional groups in the unknown Will take in lab today
Qualitative Analysis of Unknown Compounds 1. Infrared Spectroscopy Identification of functional groups in the unknown Will take in lab today 2. Elemental Analysis Determination of the Empirical Formula
More informationChapter 12 Structure Determination: Mass Spectrometry and Infrared Spectroscopy
Chapter 12 Structure Determination: Mass Spectrometry and Infrared Spectroscopy Figure 12.1 - The electron-ionization, magneticsector mass spectrometer Representing the Mass Spectrum Base Peak Parent
More informationOrganic Compound Identification Using Infrared Spectroscopy. Description
Return to paper Organic Compound Identification Using Infrared Spectroscopy Dr. Walt Volland, Bellevue Community College All rights reserved 1999, Bellevue, Washington Description This exercise is intended
More informationTopic 2.11 ANALYTICAL TECHNIQUES. High Resolution Mass Spectrometry Infra-red Spectroscopy
Topic 2.11 ANALYTICAL TECHNIQUES High Resolution Mass Spectrometry Infra-red Spectroscopy HIGH RESOLUTION MASS SPECTROMETRY The technique of mass spectrometry was used in Unit 1 to: a) determine the relative
More informationCHE 325 SPECTROSCOPY (A) CHAP 13A ASSIGN CH 2 CH CH 2 CH CHCH 3
CE 325 SPECTRSCPY (A) CAP 13A ASSIGN 1. Which compound would have a UV absorption band at longest wavelength? A. I B. II C. III D. IV E. V C CC 3 CC C 2 C CC 3 I II III C 2 C C 2 C CC 3 IV V 2. Select
More informationFri 6 Nov 09. More IR Mass spectroscopy. Hour exam 3 Fri Covers Chaps 9-12 Wednesday: Review
Fri 6 Nov 09 our exam 3 Fri 11-13 Covers Chaps 9-12 Wednesday: Review More IR Mass spectroscopy Good web site for IR, Mass, NMR spectra: http://riodb01.ibase.aist.go.jp/sdbs/cgi-bin/cre_index.cgi?lang=eng
More informationChapter Two mass spectra are shown. One corresponds to 2-methyl-2-pentene; the other, to 2-hexene. Which is which? Explain.
hapter 12 12.1 Write as many molecular formulas as you can for compounds that have the following molecular ions in their mass spectra. Assume that all the compounds contain and and that may or may not
More informationInfrared Spectroscopy: How to use the 5 zone approach to identify functional groups
Infrared Spectroscopy: How to use the 5 zone approach to identify functional groups Definition: Infrared Spectroscopy is the study of the Infrared Spectrum. An Infrared Spectrum is the plot of photon energy
More informationChemistry 343- Spring 2008
Chemistry 343- Spring 2008 27 Chapter 2- Representative Carbon Compounds: Functional Groups, Intermolecular Forces and IR Spectroscopy A. ydrocarbons: Compounds composed of only C and Four Basic Types:
More informationStructure Determination: Mass Spectrometry and Infrared Spectroscopy. What is the size and formula? Infrared spectroscopy
Revs checked MR Structure Determination: Mass Spectrometry and Infrared Spectroscopy 12 12-1 Mass Spectrometry of Small Molecules: MagneticSector Instruments 12-2 Interpreting Mass Spectra 12-3 Mass Spectrometry
More informationTable 8.2 Detailed Table of Characteristic Infrared Absorption Frequencies
Table 8.2 Detailed Table of Characteristic Infrared Absorption Frequencies The hydrogen stretch region (3600 2500 cm 1 ). Absorption in this region is associated with the stretching vibration of hydrogen
More information3 Use of Mass Spectra to Obtain Structural Information
3 Use of Mass Spectra to Obtain Structural Information 1 Mass Spectrometry One of the most sensitive and versatile analytical tools More sensitive than other spectroscopic methods (e.g. IR spectroscopy)
More informationDetermining the Structure of an Organic Compound
Chapter 12- Structure Determination: Mass Spectrometry and Infrared Spectroscopy Ashley Piekarski, Ph.D. Determining the Structure of an Organic Compound The analysis of the outcome of a reac=on requires
More informationStructure Determination
There are more than 5 million organic compounds, the great majority of which are colourless liquids or white solids. Identifying or at least characterising determining some of its properties and features
More informationGeneral Infrared Absorption Ranges of Various Functional Groups
General Infrared Absorption Ranges of Various Functional Groups Frequency Range Bond Type of Compound cm -1 Intensity C Alkanes 2850-2970 Strong 1340-1470 Strong C Alkenes 3010-3095 Medium 675-995 Strong
More informationChapter 20. Mass Spectroscopy
Chapter 20 Mass Spectroscopy Mass Spectrometry (MS) Mass spectrometry is a technique used for measuring the molecular weight and determining the molecular formula of an organic compound. Mass Spectrometry
More information1 Which of the following cannot be used to detect alcohol in a breathalyser test? Fractional distillation. Fuel cell. Infrared spectroscopy
1 Which of the following cannot be used to detect alcohol in a breathalyser test? Fractional distillation Fuel cell Infrared spectroscopy Reduction of dichromate(vi) ions 2 Propanal, H 3 H 2 HO, and propanone,
More informationNUCLEAR MAGNETIC RESONANCE AND INTRODUCTION TO MASS SPECTROMETRY
NUCLEAR MAGNETIC RESONANCE AND INTRODUCTION TO MASS SPECTROMETRY A STUDENT SHOULD BE ABLE TO: 1. Identify and explain the processes involved in proton ( 1 H) and carbon-13 ( 13 C) nuclear magnetic resonance
More information1.1. IR is part of electromagnetic spectrum between visible and microwave
CH2SWK 44/6416 IR Spectroscopy 2013Feb5 1 1. Theory and properties 1.1. IR is part of electromagnetic spectrum between visible and microwave 1.2. 4000 to 400 cm -1 (wave numbers) most interesting to organic
More informationi e l d f Energy (E) = Direction visible ultraviolet X-ray gamma infrared
rganic Structure Determination Analytical hemistry Instrument-based methods for determination of structure of organic molecules 1) Infrared Spectroscopy - yields functional groups 2) NMR Spectroscopy -
More informationChapter 13: Molecular Spectroscopy
Chapter 13: Molecular Spectroscopy Electromagnetic Radiation E = hν h = Planck s Constant (6.63 x 10-34 J. s) ν = frequency (s -1 ) c = νλ λ = wavelength (nm) Energy is proportional to frequency Spectrum
More informationChapter 9. Nuclear Magnetic Resonance. Ch. 9-1
Chapter 9 Nuclear Magnetic Resonance Ch. 9-1 1. Introduction Classic methods for organic structure determination Boiling point Refractive index Solubility tests Functional group tests Derivative preparation
More informationC h a p t e r S i x t e e n: Nuclear Magnetic Resonance Spectroscopy. An 1 H NMR FID of ethanol
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 C h a p t e r S i x t e e n: Nuclear Magnetic Resonance Spectroscopy An 1 NMR FID of ethanol Note: Problems with italicized numbers
More informationChapter 2. Molecular Representations
hapter 2. Molecular Representations 3 () 3 ( 3 ) 2 3 3 3 8 Lewis (Kekule) structure ondensed and par6ally condensed structure Skeletal (bond- line) structure Molecular formula Amoxicillin a widely prescribed
More informationChapter 2 Molecular Representations
hapter 2 Molecular Representations Structural Formulas and ondensed Structures Because organic compounds are molecular in nature, the additional information conveyed in structural formulas is sometimes
More informationOrganic Chemistry I Exam 3 Fall 2001 November 30, Which of the following compounds corresponds to the spectral data given below?
. Which of the following compounds corresponds to the spectral data given below? one of these. The reaction energy diagram given below corresponds to which of the following reactions? TS TS TS Br + R RI
More informationChem 14C Lecture 1 Spring 2016 Exam 2 Solutions Page 1
Chem 14C Lecture 1 Spring 2016 Exam 2 Solutions Page 1 Statistics: High score, average, and low score will be posted on the course web site after exam grading is complete. Some questions have more than
More informationORGANIC SPECTROSCOPY NOTES
- 1 - ORGANIC SPECTROSCOPY NOTES Basics of Spectroscopy UV/vis, IR and NMR are all types of Absorption Spectroscopy, where EM radiation corresponding to exactly the energy of specific excitations in molecules
More informationChapter 5. Mass spectrometry
ionization and fragmentation Chapter 5. Mass spectrometry which fragmentations? mass and frequency, m/z and count rate Reading: Pavia Chapters 3 and 4 Don t need 3.3 B-D, 3.4 B-D Use the text to clarify
More informationClasses of Organic Compounds
Unit 1 Functional Groups Depicting Structures of rganic ompounds Lewis Structures ondensed structural formulas Line angle drawings 3-dimensional structures Resonance Structures Acid-Base Reactions urved
More informationChem Final Examination August 7, 2004
Chem 281 2004-2 Final Examination August 7, 2004 Name: Student Number: Note: You are allowed to use models for this exam. Notes, textbooks and calculators are strictly prohibited. Write your final answers
More informationMass Spectroscopy. Dr. Sapna Gupta
Mass Spectroscopy Dr. Sapna Gupta What is Mass Spectroscopy It is an analytical technique for measuring the mass-tocharge ratio (m/z) of ions in the gas phase. Mass spectrometry is our most valuable analytical
More informationMASS and INFRA RED SPECTROSCOPY
MASS and INFRA RED SPECTRSCPY Mass Spectroscopy The mass spectrometer was looked at in Unit 1. It was noted there that compounds produce fragmentation patterns when passes through a mass spectrometer.
More informationPAPER No.12 :Organic Spectroscopy MODULE No.30: Combined problem on UV, IR, 1 H NMR, 13 C NMR and Mass - Part II
Subject Chemistry Paper No and Title Module No and Title Module Tag 12 : rganic Spectroscopy 30: Combined problem on UV, IR, 1 H NMR, 13 C NMR and Mass Part-II CHE_P12_M30 TABLE F CNTENTS 1. Learning utcomes
More informationAnswers to Assignment #5
Answers to Assignment #5 A. 9 8 l 2 5 DBE (benzene + 1 DBE) ( 9 2(9)+2-9 8+1+1 = 10 ˆ 5 DBE) nmr pattern of two doublets of equal integration at δ7.4 and 7.9 ppm means the group (the δ7.9 shift) IR band
More informationMass Spectrometry Instrumentation
Mass Spectrometry Instrumentation A mass spectrometer is composed of an inlet system (which introduces the sample to the instrument and vaporizes the sample) A molecular leak (which produces a steady stream
More informationUnit 11 Instrumentation. Mass, Infrared and NMR Spectroscopy
Unit 11 Instrumentation Mass, Infrared and NMR Spectroscopy Spectroscopic identification of organic compounds Qualitative analysis: presence but not quantity (i.e. PEDs) Quantitative analysis: quantity
More informationIR absorption spectroscopy
IR absorption spectroscopy IR spectroscopy - an analytical technique which helps determine molecules structure When a molecule absorbs IR radiation, the vibrational energy of the molecule increase! The
More informationInfrared Spectroscopy
x-rays ultraviolet (UV) visible Infrared (I) microwaves radiowaves near I middle I far I λ (cm) 8 x 10-5 2.5 x 10-4 2.5 x 10-3 2.5 x 10-2 µ 0.8 2.5 25 250 ν (cm -1 ) 13,000 4,000 400 40 ν (cm -1 1 ) =
More informationEXPT. 9 DETERMINATION OF THE STRUCTURE OF AN ORGANIC COMPOUND USING UV, IR, NMR AND MASS SPECTRA
EXPT. 9 DETERMINATION OF THE STRUCTURE OF AN ORGANIC COMPOUND USING UV, IR, NMR AND MASS SPECTRA Structure 9.1 Introduction Objectives 9.2 Principle 9.3 Requirements 9.4 Strategy for the Structure Elucidation
More informationSTRUCTURE ELUCIDATION BY INTEGRATED SPECTROSCOPIC METHODS
Miscellaneous Methods UNIT 14 STRUCTURE ELUCIDATION BY INTEGRATED SPECTROSCOPIC METHODS Structure 14.1 Introduction Objectives 14.2 Molecular Formula and Index of Hydrogen Deficiency 14.3 Structural Information
More information1. Which compound would you expect to have the lowest boiling point? A) NH 2 B) NH 2
MULTIPLE CICE QUESTINS Topic: Intermolecular forces 1. Which compound would you expect to have the lowest boiling point? A) N 2 B) N 2 C) N D) E) N Ans: : N 2 D Topic: Molecular geometry, dipole moment
More informationCHEMISTRY 341. Final Exam Tuesday, December 16, Problem 1 15 pts Problem 9 8 pts. Problem 2 5 pts Problem pts
CEMISTRY 341 Final Exam Tuesday, December 16, 1997 Name NAID Problem 1 15 pts Problem 9 8 pts Problem 2 5 pts Problem 10 21 pts Problem 3 26 pts Problem 11 15 pts Problem 4 10 pts Problem 12 6 pts Problem
More information2FAMILIES OF CARBON COMPOUNDS:
P1: PBU/VY P2: PBU/VY Q: PBU/VY T1: PBU Printer: Bind Rite JWL338-02 JWL338-Solomons-v1 April 23, 2010 21:49 2AMILIES ARB MPUDS: UTIAL GRUPS, ITERMLEULAR RES, AD IRARED (IR) SPETRSPY SLUTIS T PRBLEMS 2.1
More informationOrganic Chemistry II (CHE ) Examination I February 11, Name (Print legibly): Key. Student ID#:
rganic hemistry II (HE 232-001) Examination I February 11, 2009 Name (Print legibly): Key (last) (first) Student ID#: PLEASE observe the following: You are allowed to have scratch paper (provided by me),
More informationLecture 2 nmr Spectroscopy
Lecture 2 nmr Spectroscopy Pages 427 430 and Chapter 13 Molecular Spectroscopy Molecular spectroscopy: the study of the frequencies of electromagnetic radiation that are absorbed or emitted by substances
More informationCHEMISTRY Topic #3: Using Spectroscopy to Identify Molecules: Radicals and Mass Spectrometry (MS) Spring 2018 Dr.
CHEMISTRY 2600 Topic #3: Using Spectroscopy to Identify Molecules: Radicals and Mass Spectrometry (MS) Spring 2018 Dr. Susan Findlay Mass Spectrometry: How Does It Work? In CHEM 1000, you saw that mass
More informationCHEM Chapter 12 Infrared and Mass Spec (homework). Stafford. S18
Exhibit 12-4 The following question(s) refer to the mass spectrum shown below. 1. Refer to Exhibit 12-4. This compound contains C, H, and one other atom. Identify the other atom from the mass spectrum
More information