TLC Densitometric Quantification of Vasicine, Vasicinone and Embelin from Adhatoda zeylanica leaves and Embelia ribes fruits

Size: px
Start display at page:

Download "TLC Densitometric Quantification of Vasicine, Vasicinone and Embelin from Adhatoda zeylanica leaves and Embelia ribes fruits"

Transcription

1 Chapter 8 TLC Densitometric Quantification of Vasicine, Vasicinone and Embelin from Adhatoda zeylanica leaves and Embelia ribes fruits 8.1 INTRODUCTION With the global increase in the demand for plant derived medicine, there is a need to ensure the quality of the herbal drugs. Plants are a complex mixture of a variety of chemical constituents that can vary considerably depending on genetic and environmental factors, method of cultivation, time of collection, post-harvest processing, etc. This inherent variability in the chemistry may adversely affect the efficacy of medicinal plants. Hence it is a pre-requisite to ensure that the plants used for therapy or for research purposes are of a quality that gives the desired efficacy and that the quality is maintained at each re-collection of the plant materials. To meet this requirement it is essential to establish qualitative and quantitative chemoprofiles of the samples. The present chapter describes the TLC fingerprint profile of the methanolic extract of Adhatoda vasica leaf and Embelia ribes fruit, co-chromatography with the bioactive compounds vasicine, vasicinone and embelin respectively and its quantification using TLC densitometry EXPERIMENTAL Chemicals All solvents and chemicals used were of analytical grade Apparatus (a) Spotting device CAMAG Linomat V Automatic Sample Spotter (b) Syringe 100 µl (Hamilton) (c) TLC Chamber CAMAG glass twin trough chamber ( cm) (d) Densitometer CAMAG TLC Scanner 3 linked to wincats software 132

2 (e) TLC plates precoated silica gel 60 F 254 TLC plate (E. Merck, Cat. no ) (0.2 mm thickness) Plant material collection and authentication The leaves of Adhatoda zeylanica and fruits of Embelia ribes were collected from Gujarat, India. Their authenticity was confirmed by the taxonomist Dr. Sheetal Anandgiwala of our department and a voucher specimen was deposited at the Department of Pharmacognosy and Phytochemistry, B. V. Patel Pharmaceutical Education & Research Development (PERD) Centre, Ahmedabad, India. The material was stored in air tight containers at room temperature until use TLC analysis of Adhatoda zeylanica leaves TLC fingerprint profiling Preparation of sample solution 1 g of the powdered plant material was extracted with methanol (25 ml 2) under reflux on a water bath for 30 min. The extracts were filtered, pooled, concentrated and the volume was made up to 25 ml with methanol. Preparation of standard solutions 2 mg of vasicine (purity 98.5 %) standard was dissolved in methanol and the volume was made up to 25 ml in a volumetric flask. 2 mg of vasicinone (purity 97.5 %) standard was dissolved in methanol and the volume was made up to 25 ml in a volumetric flask. Mobile phase Ethyl acetate: Methanol: Ammonia (8: 2: 0.2) Procedure 10 µl each of the sample solution and the standard solutions were applied on a precoated silica gel 60 F 254 TLC plate (E. Merck) of uniform thickness (0.2 mm) and the plate was developed in a twin trough chamber containing mobile phase Ethyl acetate: methanol: ammonia (8: 2: 0.2) up to a distance of 8 cm at 25 ± 2 C temperature and 40 % relative humidity. The plate was dried at room temperature and scanned at 292 nm for vasicine and 233 nm for vasicinone. 133

3 The plate was observed under UV light at 254 nm and the plate was derivatized with modified Dragendorff s reagent (Wagner and Bladt, 1996). The R f and colour of the resolved bands were noted Estimation of vasicine and vasicinone using TLC densitometric method Preparation of sample solution 1 g of the powdered plant material was extracted with methanol (25 ml 2) under reflux on a water bath for 30 min. The extracts were filtered, pooled, concentrated and the volume was made up to 25 ml with methanol. Preparation of standard solutions of vasicine and vasicinone A) Standard solution of vasicine- 5 mg of vasicine standard was dissolved in 25 ml of methanol in a volumetric flask. From this stock solution standard solutions of µg/ml were prepared by transferring aliquots (1.6 to 4.8 ml) of stock solution to 10 ml volumetric flasks and adjusting the volume to 10 ml with methanol. B) Standard solution of vasicinone - A stock solution of vasicinone was prepared by dissolving 2 mg of accurately weighed vasicinone in methanol and making up the volume to 25 ml with methanol. From this stock solution standard solutions of µg/ml were prepared by transferring aliquots (0.5 to 3 ml) of stock solution to 10 ml volumetric flasks and adjusting the volume with methanol. Calibration curve for vasicine and vasicinone 10 µl of each of the standard solutions of, vasicine and vasicinone were applied in triplicate on TLC plate. The plates were developed in a solvent system of Ethyl acetate : Methanol : Ammonia (8 :2 :0.2 v/v) at 25±2 C temperature and 40 % relative humidity, upto a distance of 8 cm. After development, the plate was dried in air and scanned at 292 nm for vasicine and 233 nm for vasicinone. The peak areas were recorded. Calibration curves of vasicine and vasicinone were prepared by plotting peak areas vs concentration. Quantification of vasicine and vasicinone in the sample 10 µl of sample solution was applied in triplicate on a TLC plate. The plate was developed and scanned as described above. The peak areas were recorded. The amount 134

4 of vasicine and vasicinone in sample was calculated using the linear regression equation derived from the calibration curve of vasicine and vasicinone respectively Method validation International Conference on Harmonization (ICH) guidelines were followed for the validation of the analytical procedures (CPMP/ICH/281/95 and CPMP/ICH/381/95). The method was validated for precision, repeatability and accuracy. Instrumental precision was checked by repeated scanning of the same spot of vasicine (400 ng) and vasicinone (160 ng) seven times and was expressed as coefficient of variance (% CV). The repeatability of the method was affirmed by analyzing 400 ng/spot of standard solution of vasicine and 160 ng/spot of standard solution of vasicinone after application on the TLC plate (n = 6) and was expressed as % CV. Variability of the method was studied by analyzing aliquots of standard solution of vasicine (320, 400, 480 ng/spot) and vasicinone (160, 240, 320 ng/spot) on the same day (intra-day precision) and on different days (inter-day precision) and the results were expressed as % CV. Accuracy of the method was tested by performing recovery studies at three levels (50 %, 100 % and 125 % addition). The percent recovery and the average percent recovery were calculated. For the determination of limit of detection and limit of quantification different dilutions of the standard solutions of vasicine and vasicinone were applied along with methanol as the blank and determined on the basis of signal to noise ratio TLC analysis of Embelia ribes fruit TLC fingerprint profiling Preparation of sample solution 1 g of the powdered plant material was extracted with methanol (25 ml 2) under reflux on a water bath. The extracts were filtered, pooled, concentrated and the volume was made up to 25 ml with methanol. Preparation of standard solutions 2 mg of embelin (purity 95 %) standard was dissolved in methanol and the volume was made up to 25 ml in a volumetric flask. Mobile phase n Propanol : n Butanol : Ammonia (7 : 1 : 2) 135

5 Procedure 10 µl each of the sample solution and the standard solutions were applied on a precoated silica gel 60 F 254 TLC plate (E. Merck) of uniform thickness (0.2 mm) and the plate was developed in a twin trough chamber containing mobile phase n Propanol : n Butanol : Ammonia (7 : 1 : 2) up to a distance of 8 cm at 25 ± 2 C temperature and 40 % relative humidity. The plate was dried at room temperature and scanned at 333 nm for embelin. The plate was observed under UV light at 254 nm and the R f of the resolved bands were noted Estimation of embelin using TLC densitometric method Preparation of sample solution 1 g of the powdered plant material was extracted with methanol (25 ml 2) under reflux on a water bath for 30 min. The extracts were filtered, pooled, concentrated and the volume was made up to 25 ml with methanol. Preparation of standard solution of embelin A stock solution of embelin was prepared by dissolving 2 mg of accurately weighed embelin in methanol and making up the volume to 25 ml with methanol. From this stock solution standard solutions of µg/ml were prepared by transferring aliquots (0.5 to 3 ml) of stock solution to 10 ml volumetric flasks and adjusting the volume with methanol. Calibration curve embelin 10 µl of the standard solution embelin was applied in triplicate on TLC plate. The plates were developed in a solvent system of n Propanol : n Butanol : Ammonia (7 : 1 : 2) at 25±2 C temperature and 40 % relative humidity, up to a distance of 8 cm. After development, the plate was dried in air and scanned at 333 nm for embelin. The peak areas were recorded. Calibration curve of embelin was prepared by plotting peak areas vs concentration. Quantification of embelin in the sample 10 µl of sample solution was applied in triplicate on a TLC plate. The plate was developed and scanned as described above. The peak areas were recorded. The amount 136

6 of embelin in sample was calculated using the linear regression equation derived from the calibration curve of embelin Method validation International Conference on Harmonization (ICH) guidelines were followed for the validation of the analytical procedures (CPMP/ICH/281/95 and CPMP/ICH/381/95). The method was validated for precision, repeatability and accuracy. Instrumental precision was checked by repeated scanning of the same spot of emeblin (320 ng) was expressed as coefficient of variance (% CV). The repeatability of the method was affirmed by analyzing 320 ng/spot of standard solution of embelin after application on the TLC plate (n = 6) and was expressed as % CV. Variability of the method was studied by analyzing aliquots of standard solution of embelin (160, 240, 320 ng/spot) on the same day (intra-day precision) and on different days (inter-day precision) and the results were expressed as % CV. Accuracy of the method was tested by performing recovery studies at three levels (50 %, 100 % and 125 % addition). The percent recovery and the average percent recovery were calculated. For the determination of limit of detection and limit of quantification different dilutions of the standard solutions of vasicine and vasicinone were applied along with methanol as the blank and determined on the basis of signal to noise ratio RESULTS AND DISCUSSION TLC fingerprint profiling of Adhatoda zeylanica leaves The constituents other than the biomarkers may be contributing to the therapeutic effect of the plant either by synergizing or adding to the effect of the active ingredients, or by antagonizing their adverse effects, or by potentiating their bioavailability (Hamburger and Hostettman, 1991) Therefore, as far as the quality of a medicinal plant drug is concerned, a systematic consideration of all its phytoconstituents is as important as the quantification of the active constituents present in it. TLC fingerprint profile is a systematic documentation of all the constituents of a sample extract resolved in a given chromatographic system. It provides a semi-quantitative sketch of the chemoprofile of the plant extract. The methanolic extract of Adhatoda zeylanica leaf was used for TLC fingerprint profiling and co-tlc with vasicine and vasicinone. The methanolic extract showed the 137

7 best possible resolution of its constituents on precoated silica gel TLC plates, when developed in mobile phase containing Ethyl acetate : Methanol : Ammonia (8 :2 :0.2 v/v). The developed TLC plate, when observed under UV light showed bands at 254 nm and R f value of which are mentioned in (Table 8.1; Figure 8.1). Table 8.1: TLC details of sample solution of Adhatoda zeylanica leaf. Under UV light at 254 nm (R f value) (Vasicine) (Vasicinone) Quantification of biomarkers vasicine and vasicinone , Figure 8.1 TLC profile of Adhatoda zeylanica leaf. 1: Vasicine standard (0.8µg) ; 2: Vasicinone standard (0.8µg); 3,4,5: Sample solution (0.4µg, 0.8µg, 1.6µg) As far as the analysis of herbal raw materials and herbal preparations is concerned, HPTLC has an edge over other instrumental analytical techniques because it is simple, economical and requires minimum sample clean-up. That is why HPTLC has emerged as an efficient tool for the phytochemical evaluation of herbal drugs (Rajani et al., 2001; Quality Standards of Indian Medicinal Plants, 2003). A densitometric technique scanned at 298 nm and 233 nm was therefore adopted for the quantification of vasicine and vasicinone respectively in the leaf of Adhatoda zeylanica leaf using HPTLC. Of the various solvent systems tried, the one containing Ethyl acetate: Methanol: Ammonia (8 : 2 : 0.5 v/v) gave the best resolution of vasicine (R f = 0.62) and vasicinone (R f = 0.81) in the presence of other compounds in the sample extract. The methods were validated in terms of precision, repeatability and accuracy (Table 8.2). The linear relationship between peak area and amount of vasicine applied was found only within the range of ng/spot with correlation coefficient and standard deviation The linear relationship between peak area and amount of vasicinone applied was found only within the range of ng/spot with correlation coefficient and standard deviation 3.62.The intra-day and inter-day precision 138

8 expressed as % CV (Table 8.3) indicate that the proposed method was precise and reproducible. The limit of detection for vasicine was found to be 40 ng and the limit of quantification was found to be 80 ng. The average of percentage recovery at three different levels was found to be % (Table 8.3). The limit of detection for vasicinone was found to be 20 ng and the limit of quantification was found to be 40 ng. The average of percentage recovery at three different levels was found to be % (Table 8.4). The amount of vasicine and vasicinone in Adhatoda zeylanica leaf was found to be 1.25 % (w/w) and % (w/w) respectively as quantified by the proposed method. A B Figure 8.2: A. TLC densitometric scan at 298 nm of Adhatoda zeylanica leaf methanolic extract (blue line) along with vasicine standard (purple line). B. Overlay of UV absorption spectra of the vasicine in the sample track with the vasicine standard A B Figure 8.3: A. TLC densitometric scan at 233 nm of Adhatoda zeylanica leaf methanolic extract (orange line) along with vasicinone standard (pink line) B. Overlay of UV absorption spectra of the vasicinone in the sample track with the vasicinone standard 139

9 Table 8.2: Method validation parameters for the quantification of vasicine and vasicinone by the proposed method Parameters Vasicine Vasicinone Instrumental precision (% CV, n = 7) Repeatability of standards (% CV, n = 6) Accuracy (average % recovery) Limit of detection 40 ng 20 ng Limit of quantification 80 ng 40 ng Specificity Specific Specific Linearity (Correlation coefficient) Range (ng/spot) Table 8.3: Intra-day and Inter-day precision of vasicine and vasicinone Marker compound Concentration Intra-day Inter-day (ng spot -1 ) Precision Precision Vasicine Vasicinone

10 Table 8.4 Recovery study of vasicine and vasicinone by the proposed TLC densitometric method Marker compound Amount present in the sample (µg) Amount added (µg) Amount found a (µg) Recovery a (%) Average recovery (%) Vasicine Vasicinone ± ± ± ± ± ± ± ± ± ± ± ± a Mean ± Standard deviation, (n = 3) TLC fingerprint profiling of Embelia ribes fruits The methanolic extract of Embelia ribes fruit leaf was used for TLC fingerprint profiling and co-tlc with embelin. The methanolic extract showed the best possible resolution of its constituents on precoated silica gel TLC plates, when developed in mobile phase containing n-propanol : n-butanol : Ammonia (7 : 1 : 2 v/v). The developed TLC plate, when observed under UV light showed bands at 254 nm and R f value of which are mentioned in (Figure 8.4). Table 8.5: TLC details of sample solution of Embelia ribes fruit Under UV light at 254 nm (R f value) 0.35 (Embelin) Figure 8.4: TLC profile of Embelia ribes fruit. 1: Embelin; 2: Sample solution 141

11 8.3.4 Quantification of biomarker embelin Of the various solvent systems tried, the one containing n-propanol: n-butanol: Ammonia (7 : 1 : 2 v/v) gave the best resolution of embelin (R f = 0.35) in the presence of other compounds in the sample extract. The methods were validated in terms of precision, repeatability and accuracy (Table 8.6). The linear relationship between peak area and amount of embelin applied was found only within the range of ng/spot with correlation coefficient and standard deviation The intra-day and inter-day precision expressed as % CV (Table 8.7) indicate that the proposed method was precise and reproducible. The limit of detection for embelin was found to be 20 ng and the limit of quantification was found to be 40 ng. The average of percentage recovery at three different levels was found to be % (Table 8.8). The amount of embelin in Embelia ribes fruit was found to be 3.21 % w/w as quantified by the proposed method. A B Figure 8.5 A. TLC densitometric scan at 337 nm of Embelia ribes fruit methanolic extract (blue line) along with embelin standard (purple line). B. Overlay of UV absorption spectra of the embelin in the sample track with the embelin standard. 142

12 Table 8.6: Method validation parameters for the quantification of embelin by the proposed method Parameters Embelin Instrumental precision 0.83 (% CV, n = 7) Repeatability of standards 0.46 (% CV, n = 6) Accuracy (average % recovery) Limit of detection 20 ng Limit of quantification 40 ng Specificity Specific Linearity (Correlation coefficient) Range (ng/spot) Table 8.7 Intra-day and Inter-day precision of embelin Marker compound Embelin Concentration (ng spot -1 ) Intra-day precision a Inter-day precision a Table 8.8 Recovery study of embelin by the proposed TLC densitometric method Marker compound Amount present in the sample (µg) Amount added (µg) Amount found a (µg) Recovery a (%) Average recovery (%) Embelin ± ± ± ± ± ± a Mean ± Standard deviation, (n = 3) 143

13 8.4 CONCLUSION The developed HPTLC methods for the quantification of vasicine, vasicinone and embelin were found to be simple, precise, specific and sensitive. The amount of vasicine and vasicinone in Adhatoda zeylanica leaf was found to be 1.25 and % (w/w) respectively. The amount of embelin in Embelia ribes fruit was found to be 3.21 % (w/w). 144

Development and validation of HPTLC method for analysis of ursolic acid from methanol extract of mimosoups elengi L

Development and validation of HPTLC method for analysis of ursolic acid from methanol extract of mimosoups elengi L Online at www.pharmaresearchlibrary.com/jpbmal JPBMAL, 2013: Vol.1(1), 5-9 Development and validation of HPTLC method for analysis of ursolic acid from methanol extract of mimosoups elengi L Gayatri Ganu*

More information

SIMULTANEOUS ESTIMATION OF CILOSTAZOL AND ASPIRIN IN SYNTHETIC MIXTURE USING HPTLC METHOD

SIMULTANEOUS ESTIMATION OF CILOSTAZOL AND ASPIRIN IN SYNTHETIC MIXTURE USING HPTLC METHOD Int. J. Chem. Sci.: 6(3), 2008, 1377-1384 SIMULTANEOUS ESTIMATION OF CILOSTAZOL AND ASPIRIN IN SYNTHETIC MIXTURE USING HPTLC METHOD JAYESH V. PATEL, C. N. PATEL, P. U. PATEL a PANKAJ H. PRAJAPATI, I. S.

More information

International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : Vol.2, No.2, pp , April-June 2010

International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : Vol.2, No.2, pp , April-June 2010 International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.2, No.2, pp 851-855, April-June 2010 Development and Validation of HPTLC Method for Determination of Betulinic Acid in

More information

Scholars Research Library. Der Pharmacia Lettre, 2015, 7 (5): (http://scholarsresearchlibrary.com/archive.html)

Scholars Research Library. Der Pharmacia Lettre, 2015, 7 (5): (http://scholarsresearchlibrary.com/archive.html) Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (5):300-306 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

QUANTITATION OF EMBELIN FROM PLANT EMBELIA DRUPACEA (DENNST) M. R & S. M. ALMEIDA BY DEVELOPMENT AND VALIDATION OF H.P.T.L.C.

QUANTITATION OF EMBELIN FROM PLANT EMBELIA DRUPACEA (DENNST) M. R & S. M. ALMEIDA BY DEVELOPMENT AND VALIDATION OF H.P.T.L.C. Page130 IJPBS Volume 5 Issue 1 JAN-MAR 2015 130-137 Research Article Biological Sciences QUANTITATION OF EMBELIN FROM PLANT EMBELIA DRUPACEA (DENNST) M. R & S. M. ALMEIDA BY DEVELOPMENT AND VALIDATION

More information

Development and Validation of HPTLC Method for the Estimation of Repaglinide in Bulk and Tablet Dosage Form

Development and Validation of HPTLC Method for the Estimation of Repaglinide in Bulk and Tablet Dosage Form Research Article www.pharmaresearchlibrary.com/ijctpr ISSN: 2321-3760 International Journal of Current Trends in Pharmaceutical Research IJCTPR, 2013: Vol. 1(3): 189-193 Development and Validation of HPTLC

More information

Pelagia Research Library

Pelagia Research Library Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2012, 3 (4):457-461 ISSN: 0976-8688 CODEN (USA): PSHIBD Development and validation of HPTLC method for estimation of fluvastatin

More information

Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune , India

Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune , India ACTA CHROMATOGRAPHICA, NO. 19, 2007 DEVELOPMENT AND VALIDATION OF A METHOD FOR SIMULTANEOUS DENSITOMETRIC ESTIMATION OF ATORVASTATIN CALCIUM AND EZETIMIBE AS THE BULK DRUG AND IN TABLET DOSAGE FORMS S.

More information

CHAPTER 5 PHYTOCHEMICAL EVALUATION

CHAPTER 5 PHYTOCHEMICAL EVALUATION 76 CHAPTER 5 PHYTOCHEMICAL EVALUATION Phytochemistry is mainly concerned with enormous varieties of secondary plant metabolites which are biosynthesized by plants. The plant kingdom represents a treasure

More information

Journal of Chemical and Pharmaceutical Research, 2012, 4(10): Research Article

Journal of Chemical and Pharmaceutical Research, 2012, 4(10): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(10):4570-4574 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of HPTLC Method for

More information

QUANTITATION OF LUPEOL OCTACOSANOATE. HEMIDESMUS INDICUS R. Br. ROOT POWDER BY HPTLC

QUANTITATION OF LUPEOL OCTACOSANOATE. HEMIDESMUS INDICUS R. Br. ROOT POWDER BY HPTLC IN HEMIDESMUS INDICUS R. Br. ROOT POWDER BY HPTLC Rahul S. Darekar, Amol B. Khetre, Purushottam K. Sinha, Rajesh M. Jeswani and Mrinalini C. Damle* *Department of Pharmaceutical Chemistry, AISSMS College

More information

Phytochemical and HPTLC Studies of Various Extracts of Annona squamosa (Annonaceae).

Phytochemical and HPTLC Studies of Various Extracts of Annona squamosa (Annonaceae). International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.1, pp 364-368, Jan-Mar 2012 Phytochemical and HPTLC Studies of Various Extracts of Annona squamosa (Annonaceae).

More information

WORLD JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH

WORLD JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH wjpmr, 2016,2(5), 122-127. SJIF Impact Factor: 3.535 WORLD JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH www.wjpmr.com Research Article ISSN 2455-3301 WJPMR DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING

More information

Available online Research Article

Available online   Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(4):1246-1252 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of Stability-indicating

More information

METHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS

METHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS CHAPTER 6 METHOD DEVELOPMENT AND VALIDATION OF RALTEGRAVIR POTASSIUM AND RILPIVIRINE HCL BY HPLC AND HPTLC METHODS School of Pharmaceutical Sciences, Vels University 106 METHOD DEVELOPMENT AND VALIDATION

More information

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.8, No.3, pp , 2015

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.8, No.3, pp , 2015 International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.8, No.3, pp 366-370, 2015 HPTLC Fingerprinting of Scaevola frutescens Leaves as a Quality Control Parameter Raval Chirag

More information

Development and Validation of HPTLC Method for Estimation of Azilsartan Medoxomil in Bulk and Pharmaceutical Dosage Forms

Development and Validation of HPTLC Method for Estimation of Azilsartan Medoxomil in Bulk and Pharmaceutical Dosage Forms Human Journals Research Article February 2015 Vol.:2, Issue:3 All rights are reserved by Raja Gorla et al. Development and Validation of HPTLC Method for Estimation of Azilsartan Medoxomil in Bulk and

More information

Journal of Chemical and Pharmaceutical Research, 2017, 9(11): Research Article

Journal of Chemical and Pharmaceutical Research, 2017, 9(11): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(11):39-45 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development of Validated High Performance Thin Layer

More information

Research Article. Validated HPTLC method for simultaneous estimation of Aceclofenac and Pantoprazole in bulk and tablets dosage form

Research Article. Validated HPTLC method for simultaneous estimation of Aceclofenac and Pantoprazole in bulk and tablets dosage form Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(4):1180-1186 Research Article ISSN : 0975-7384 CDEN(USA) : JCPRC5 Validated HPTLC method for simultaneous estimation

More information

DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING HPTLC METHOD FOR ANALYSIS OF ANTITUBERCULAR DRUGS

DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING HPTLC METHOD FOR ANALYSIS OF ANTITUBERCULAR DRUGS ACTA CHROMATOGRAPHICA, NO. 18, 2007 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING HPTLC METHOD FOR ANALYSIS OF ANTITUBERCULAR DRUGS J. Ali *, N. Ali, Y. Sultana, S. Baboota, and S. Faiyaz Department

More information

HPTLC Fingerprint Analysis of Bastard Oleaster (Elaeagnus latifolia Linn): An Edible Plant Used by the Tribal Community of Northeast India

HPTLC Fingerprint Analysis of Bastard Oleaster (Elaeagnus latifolia Linn): An Edible Plant Used by the Tribal Community of Northeast India Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2016; 8(12); 1920-1925 Research Article ISSN: 0975-4873 HPTLC Fingerprint Analysis of Bastard Oleaster

More information

Journal of Chemical and Pharmaceutical Research, 2012, 4(6): Research Article

Journal of Chemical and Pharmaceutical Research, 2012, 4(6): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(6): 3324-3328 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 High Performance Thin Layer Chromatographic Determination

More information

Scholars Research Library

Scholars Research Library Available online at www.scholarsresearchlibrary.com Scholars Research Library Annals of Biological Research, 20, 1 (1) : 124-129 (http://scholarsresearchlibrary.com/archive.html) HPTLC method development

More information

International Journal of Pharmaceutical Sciences and Drug Research 2016; 8(4):

International Journal of Pharmaceutical Sciences and Drug Research 2016; 8(4): Available online at www.ijpsdr.com International Journal of Pharmaceutical Sciences and Drug Research 2016; 8(4): 218-222 Research Article ISSN: 0975-248X CODEN (USA): IJPSPP Development and Validation

More information

Development and validation a RP-HPLC method: Application for the quantitative determination of quetiapine fumarate from marketed bulk tablets

Development and validation a RP-HPLC method: Application for the quantitative determination of quetiapine fumarate from marketed bulk tablets Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(1):142-146 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and validation a RP-HPLC method: Application

More information

Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur , India 2

Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur , India 2 Chromatography Research International Volume 212, Article ID 27364, 6 pages doi:1.1155/212/27364 Research Article Development and Validation of a Stability-Indicating HPTLC Method for Analysis of Rasagiline

More information

This method describes the identification of the following prohibited colorants in cosmetic products:

This method describes the identification of the following prohibited colorants in cosmetic products: A. IDENTIFICATION BY TLC 1. SCOPE AND FIELD OF APPLICATION This method describes the identification of the following prohibited colorants in cosmetic products: Names C I number Pigment Orange 5 12075 Metanil

More information

Department of Chemistry, Lafayette College, Easton, PA 18042, USA

Department of Chemistry, Lafayette College, Easton, PA 18042, USA ACTA CHROMATOGRAPHICA, NO. 13, 2003 DEVELOPMENT AND VALIDATION OF A HIGH- PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD WITH DENSITOMETRIC DETECTION FOR DETERMINATION OF BISACODYL IN PHARMACEUTICAL TABLETS

More information

IDENTIFICATION OF STEROIDS IN COSMETIC PRODUCTS BY TLC AND HPLC 1 02/12/2005 ACM 007 A. THIN LAYER CHROMATOGRAPHY (TLC)

IDENTIFICATION OF STEROIDS IN COSMETIC PRODUCTS BY TLC AND HPLC 1 02/12/2005 ACM 007 A. THIN LAYER CHROMATOGRAPHY (TLC) Document A. THIN LAYER CHROMATOGRAPHY (TLC) 1. SCOPE AND FIELD OF APPLICATION The method describes the identification of hydrocortisone acetate, dexamethasone, betamethasone, betamethasone 17-valerate

More information

Ishpreet Kaur 1*, Sharad Wakode 2, Harsharan Pal Singh 3 ABSTRACT ARTICLE INFO

Ishpreet Kaur 1*, Sharad Wakode 2, Harsharan Pal Singh 3 ABSTRACT ARTICLE INFO Journal of Applied Pharmaceutical Science Vol. 6 (05), pp. 051-057, May, 16 Available online at http://www.japsonline.com DOI: 10.7324/JAPS.16.60508 ISSN 2231-3354 Development and Validation of a Stability-Indicating

More information

Validated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form

Validated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.3, pp 1228-1232, July-Sept 2012 Validated RP-HPLC Method for Estimation of Cefprozil in Tablet Dosage Form Manzoor

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(5): 92-96 ISSN No: 0975-7384 CODEN(USA): JCPRC5 High Performance Thin Layer Chromatographic

More information

Development and Validation of Stability Indicating HPTLC Method for Estimation of Mifepristone

Development and Validation of Stability Indicating HPTLC Method for Estimation of Mifepristone Research Article Development and Validation of Stability Indicating HPTLC Method for Estimation of Mifepristone D. D. Chavan, * M. C. Damle. Department of Quality Assurance, AISSMS College of Pharmacy,

More information

INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY

INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article SIMULTANEOUS DETERMINATION OF ONDANSETRON HYDROCHLORIDE AND OMEPRAZOLE IN TABLETS BY PLANAR

More information

Chapter 3: Biochemical analysis of Aloe vera L. populations through High Performance Thin Layer Chromatography (HPTLC) using Aloin as biomarker

Chapter 3: Biochemical analysis of Aloe vera L. populations through High Performance Thin Layer Chromatography (HPTLC) using Aloin as biomarker Chapter 3: Biochemical analysis of Aloe vera L. populations through High Performance Thin Layer Chromatography (HPTLC) using Aloin as biomarker 24 3.1. Introduction Medicinal plants are living resource,

More information

DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR DETERMINATION OF REBAMIPIDE FROM ITS TABLET DOSAGE FORM

DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR DETERMINATION OF REBAMIPIDE FROM ITS TABLET DOSAGE FORM Innovare Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 6, Issue 5, 2014 Original Article DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER

More information

Development and Validation of Analytical Methods for Simultaneous Estimation of Atorvastatin Calcium and Ezetimibe in Combined Dosage Form

Development and Validation of Analytical Methods for Simultaneous Estimation of Atorvastatin Calcium and Ezetimibe in Combined Dosage Form World Applied Sciences Journal 9 (2): 424-429, 200 ISSN 88-4952 IDOSI Publications, 200 Development and Validation of Analytical Methods for Simultaneous Estimation of Atorvastatin Calcium and Ezetimibe

More information

Research Article Development and Validation of HPTLC Method for Simultaneous Estimation of Paracetamol and Pamabrom in Bulk and Synthetic Mixture

Research Article Development and Validation of HPTLC Method for Simultaneous Estimation of Paracetamol and Pamabrom in Bulk and Synthetic Mixture Research Article Development and Validation of HPTLC Method for Simultaneous Estimation of Paracetamol and Pamabrom in Bulk and Synthetic Mixture MT. Harde *, RK. Chinchole and PD. Chaudhari P. E Society

More information

Optimization and validation of reverse phase HPLC and HPTLC method for simultaneous quantification of vasicine and vasicinone in Sida Species

Optimization and validation of reverse phase HPLC and HPTLC method for simultaneous quantification of vasicine and vasicinone in Sida Species Journal of Medicinal Plants Research Vol. 4(13), pp. 1289-1296, 4 July, 2010 Available online at http://www.academicjournals.org/jmpr ISSN 1996-0875 2010 Academic Journals Full Length Research Paper Optimization

More information

Received: ; Accepted:

Received: ; Accepted: International Journal of Universal Pharmacy and Bio Sciences 1(2): November-December2012 INTERNATIONAL JOURNAL OF UNIVERSAL PHARMACY AND BIO SCIENCES Pharmaceutical Sciences Research Article!!! Received:

More information

STABILITY-INDICATING HPTLC METHOD FOR ESTIMATION OF GUAIFENESIN IN BULK AND IN PHARMACEUTICAL FORMULATION

STABILITY-INDICATING HPTLC METHOD FOR ESTIMATION OF GUAIFENESIN IN BULK AND IN PHARMACEUTICAL FORMULATION ORIGINAL RESEARCH STABILITY-INDICATING HPTLC METHOD FOR ESTIMATION OF GUAIFENESIN IN BULK AND IN PHARMACEUTICAL FORMULATION P.S. Jain*, N.K. Kale, K. M. Agrawal, N. G. Haswani, S.J. Surana R. C. Patel

More information

Pelagia Research Library. Densitometric development and validation of mupirocin in ointment dosage form

Pelagia Research Library. Densitometric development and validation of mupirocin in ointment dosage form Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2013, 4(6):10-15 ISSN: 0976-8688 CODEN (USA): PSHIBD Densitometric development and validation of mupirocin in ointment dosage form

More information

Journal of Innovation in Pharmaceutical Sciences

Journal of Innovation in Pharmaceutical Sciences Journal of Innovation in Pharmaceutical Sciences Journal Home Page:www.innovationjournals.com/index-ps.html Development and validation of HPTLC method for estimation of Darifenacin Hydro bromide in Pharmaceutical

More information

A Comparative study on pytoconstitutents of Punica granatum flowers of Normal and Ornamental variety using TLC/HPTLC methods

A Comparative study on pytoconstitutents of Punica granatum flowers of Normal and Ornamental variety using TLC/HPTLC methods Journal of Scientific and Innovative Research 2016; 5(3): 87-91 Available online at: www.jsirjournal.com Research Article ISSN 2320-4818 JSIR 2016; 25(3): 87-91 2016, All rights reserved Received: 12-05-2016

More information

Scholars Research Library. Der Pharmacia Lettre, 2016, 8 (8): (http://scholarsresearchlibrary.com/archive.html)

Scholars Research Library. Der Pharmacia Lettre, 2016, 8 (8): (http://scholarsresearchlibrary.com/archive.html) Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (8):291-301 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

CHAPTER - 3 ANALYTICAL PROFILE. 3.1 Estimation of Drug in Pharmaceutical Formulation Estimation of Drugs

CHAPTER - 3 ANALYTICAL PROFILE. 3.1 Estimation of Drug in Pharmaceutical Formulation Estimation of Drugs CHAPTER - 3 ANALYTICAL PROFILE 3.1 Estimation of Drug in Pharmaceutical Formulation 3.1.1 Estimation of Drugs ANALYTICAL PROFILE 84 3.1 ESTIMATION OF DRUG IN PHARMACEUTICAL FORMULATION. Agrawal A et al

More information

Analytical method development and validation of gabapentin in bulk and tablet dosage form by using UV spectroscopic method

Analytical method development and validation of gabapentin in bulk and tablet dosage form by using UV spectroscopic method IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: www.ijpar.com ISSN:2320-2831 Research article Open Access Analytical method development and validation of gabapentin in bulk and tablet dosage

More information

Zero And First Order Derivative Spectrophotometric Methods For Determination Of Dronedarone In Pharmaceutical Formulation

Zero And First Order Derivative Spectrophotometric Methods For Determination Of Dronedarone In Pharmaceutical Formulation International Journal of PharmTech Research CDEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.1, pp 217-221, Jan-Mar 2013 Zero And First rder Derivative Spectrophotometric Methods For Determination f Dronedarone

More information

Appendix II- Bioanalytical Method Development and Validation

Appendix II- Bioanalytical Method Development and Validation A2. Bioanalytical method development 1. Optimization of chromatographic conditions Method development and optimization of chromatographic parameters is of utmost important for validating a method in biological

More information

Validated HPTLC Method for Simultaneous Quantitation of Aceclofenac, Paracetamol and Thiocolchicoside in Bulk drug and Pharmaceutical Formulation

Validated HPTLC Method for Simultaneous Quantitation of Aceclofenac, Paracetamol and Thiocolchicoside in Bulk drug and Pharmaceutical Formulation Original Article Pharm Sci Asia 2017; 44 (2), 108-114 Validated HPTLC Method for Simultaneous Quantitation of Aceclofenac, Paracetamol and Thiocolchicoside in Bulk drug and Pharmaceutical Formulation J.

More information

Ondansetron Hydrochloride Tablets

Ondansetron Hydrochloride Tablets Ondansetron Hydrochloride Tablets Dissolution Perform the test with 1 tablet of Ondansetron Hydrochloride Tablets at 50 revolutions per minute according to the Paddle method, using 900 ml of water

More information

MS, India. 2Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune , MS,

MS, India. 2Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune , MS, Received on: 25/04/2014 Accepted on: 30/05/2014 Published on: 23/06/2014 A.K. Kolsure JSPM S Jaywantrao Sawant College of Pharmacy and Research, Hadapsar, Pune- 411028, MS, India. QR Code for Mobile users

More information

J Pharm Sci Bioscientific Res (4): ISSN NO

J Pharm Sci Bioscientific Res (4): ISSN NO Development and Validation of Stability Indicating Analytical Method for Simultaneous Estimation of Perindopril and Potassium in Their Combined Marketed Dosage Form ABSTRACT: Gurjeet Kaur*, Nikhil Patel

More information

Impact factor: 3.958/ICV: 4.10 ISSN:

Impact factor: 3.958/ICV: 4.10 ISSN: Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 99 Pharma Science Monitor 9(4), Oct-Dec 2018 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com

More information

Phytochemical Fingerprinting through Computerized HPTLC System for Quality Control of Herbal Drugs

Phytochemical Fingerprinting through Computerized HPTLC System for Quality Control of Herbal Drugs Phytochemical Fingerprinting through Computerized HPTLC System for Quality Control of Herbal Drugs Alok Kumar Chandrakar 1 1 Assistant Professor, Department of Forestry, Wildlife & Environmental Sciences,

More information

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE DEVELOPMENT OF VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF BETAXOLOL HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM SIDHARTH

More information

INTERNATIONAL RESEARCH JOURNAL OF PHARMACY

INTERNATIONAL RESEARCH JOURNAL OF PHARMACY INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 8407 Research Article DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF METOPROLOL SUCCINATE AND ATORVASTATIN

More information

IDENTIFICATION AND DETERMINATION OF HYDROQUINONE IN COSMETIC PRODUCTS 2 14/11/17 ACM 003 BY TLC AND HPLC

IDENTIFICATION AND DETERMINATION OF HYDROQUINONE IN COSMETIC PRODUCTS 2 14/11/17 ACM 003 BY TLC AND HPLC A. IDENTIFICATION BY TLC 1. SCOPE AND FIELD OF APPLICATION The method describes the identification of hydroquinone in cosmetic products. 2. PRINCIPLE Hydroquinone is identified by thin layer chromatography

More information

Available online through

Available online through Research Article Available online through www.ijrap.net DENSITOMETRIC HPTLC METHOD FOR ANALYSIS OF OLEANOLIC ACID IN MENTHA PIPERITA L Rais Iram* 1, Ali Mohammed 2 1 Department of Bio-chemistry, Faculty

More information

Impact factor: 3.958/ICV: 4.10 ISSN:

Impact factor: 3.958/ICV: 4.10 ISSN: Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 62 Pharma Science Monitor 8(3), Jul-Sep 2017 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com

More information

Validated HPTLC Method for the Determination of Two Novel steroids in Bulk and Pressurized Metered-Dose Preparations

Validated HPTLC Method for the Determination of Two Novel steroids in Bulk and Pressurized Metered-Dose Preparations International Journal of Applied Science and Engineering 2011. 9, 3: 177-185 Validated HPTLC Method for the Determination of Two Novel steroids in Bulk and Pressurized Metered-Dose Hiral N. Dave a, Ashlesha

More information

Department of Chemistry, JNTUACE, Kalikiri

Department of Chemistry, JNTUACE, Kalikiri Method Development and Validation of Aegle marmeleous M. Swetha 1*, N. Saritha 1, N. Devanna 2 1 Department of Chemistry, JNTUACE, Kalikiri.-517234 2 Department of Chemistry, JNTUA, Anthapuramu -515002

More information

A Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation

A Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation Pharmaceutical Research A Simple, Novel Validated Stability Indicating RP-HPLC method for estimation of Duloxetine HCl in Capsule Pharmaceutical Formulation Manisha Puranik* a, Sailesh Wadher b and Kritika

More information

Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form

Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form Research Journal of Recent Sciences ISSN 2277-252 Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form Abstract Rele Rajan

More information

Volume 6, Issue 2, January February 2011; Article-015

Volume 6, Issue 2, January February 2011; Article-015 Research Article DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE DETERMINATION OF DAPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION USING AN EXPERIMENTAL DESIGN Pratik Mehta*, Ujjwal Sahoo,

More information

Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method

Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method 21 Article Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method Mali Audumbar Digambar*, Jadhav Santosh, Mane Pandurang,

More information

Reverse Phase High Performance Liquid Chromatography method for determination of Lercanidipine hydrochloride in bulk and tablet dosage form

Reverse Phase High Performance Liquid Chromatography method for determination of Lercanidipine hydrochloride in bulk and tablet dosage form Research Article ISSN: 0974-6943 M.V.Kumudhavalli et al. / Journal of Pharmacy Research 2014,8(11), Available online through http://jprsolutions.info Reverse Phase High Performance Liquid Chromatography

More information

Spectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form

Spectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form Spectroscopic Method For Estimation of Atorvastatin Calcium in Tablet Dosage Form Kailash P Prajapati *, A Bhandari INDO GLOBAL JOURNAL OF PHARMACEUTICAL SCIENCES ISSN 2249-1023 Faculty of Pharmaceutical

More information

Chapter-4 EXPERIMENTAL WORK BY RP-HPLC

Chapter-4 EXPERIMENTAL WORK BY RP-HPLC Chapter-4 EXPERIMENTAL WORK BY RP-HPLC 4.0 EXPERIMENTAL WORK BY RP-HPLC 4.1. DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR THE DETERMINATION OF NIFLUMIC ACID 4.1.1. MATERIALS AND METHODS OF NIFLUMIC

More information

HPTLC METHOD FOR QUANTIFICATION OF GARCINOL FROM DRY FRUIT RINDS OF GARCINIA INDICA AND ITS MARKET FORMULATION.

HPTLC METHOD FOR QUANTIFICATION OF GARCINOL FROM DRY FRUIT RINDS OF GARCINIA INDICA AND ITS MARKET FORMULATION. WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Sindhu et al. SJIF Impact Factor 2.786 Volume 4, Issue 03, 595-604. Research Article ISSN 2278 4357 HPTLC METHOD FOR QUANTIFICATION OF GARCINOL FROM

More information

Sci Pharm

Sci Pharm Sci Pharm www.scipharm.at Research article Open Access High-Performance Liquid Chromatographic and High-Performance Thin-Layer Chromatographic Method for the Quantitative Estimation of Dolutegravir Sodium

More information

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW

CHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW 75 CHAPTER 3 DEVELOPMENT AND APPLICATION OF STABILITY-INDICATING HPLC METHOD FOR THE DETERMINATION OF NEVIRAPINE AND ITS IMPURITIES IN COMBINATION DRUG PRODUCT 3.1 INTRODUCTION OF DOSAGE FORM AND LITERATURE

More information

Chapter 4: Verification of compendial methods

Chapter 4: Verification of compendial methods Chapter 4: Verification of compendial methods Introduction In order to ensure accurate and reliable test results, the quality control laboratory (QCL) needs to use analytical methods (and accompanying

More information

Analytical method development and validation of carvedilol in bulk and tablet dosage form by using uv spectroscopic method as per ich guidelines

Analytical method development and validation of carvedilol in bulk and tablet dosage form by using uv spectroscopic method as per ich guidelines IJPAR Vol.6 Issue 2 April - June -2017 Journal Home page: ISSN:2320-2831 Research article Open Access Analytical method development and validation of carvedilol in bulk and tablet dosage form by using

More information

Development and Validation of HPTLC Method for. Simultaneous Estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in Combined Dosage Form;

Development and Validation of HPTLC Method for. Simultaneous Estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in Combined Dosage Form; 142 Research Article Development and Validation of HPTLC Method for Simultaneous Estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in Combined Dosage Form Chetan A. Prajapati*, Bhavik S. Patel,

More information

Research Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC

Research Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC ISSN 2395-3411 Available online at www.ijpacr.com 248 Research Article METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR BY RP-HPLC Mantha Vebkatesh* and A. Yasodha

More information

RP-HPTLC method for determination of Voriconazole in bulk and in cream formulation

RP-HPTLC method for determination of Voriconazole in bulk and in cream formulation Arabian Journal of Chemistry (2017) 10, S355 S360 King Saud University Arabian Journal of Chemistry www.ksu.edu.sa www.sciencedirect.com ORIGINAL ARTICLE RP-HPTLC method for determination of Voriconazole

More information

Development and validation of HPTLC densitometric method for estimation of charantin in Momordica charantia fruits and herbal formulation

Development and validation of HPTLC densitometric method for estimation of charantin in Momordica charantia fruits and herbal formulation 2014; 2 (5): 172-176 ISSN 2278-4136 ISSN 2349-8234 JPP 2014; 2 (5): 172-176 Received: 18-12-2013 Accepted: 02-01-2014 Javed Ahamad* Pharmacognosy and Phytochemistry, Nagar, New Delhi, India. Saima Amin

More information

DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION

DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION Page186 Research Article Pharmaceutical Sciences DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DRONEDARONE IN BULK DRUG AND PHARMACEUTICAL FORMULATION Kishore Konam 1 &

More information

Isolation of compound (CA-01) from the bark of Careya arborea by using column chromatography

Isolation of compound (CA-01) from the bark of Careya arborea by using column chromatography 2018; 7(2): 3695-3702 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(2): 3695-3702 Received: 01-01-2018 Accepted: 02-02-2018 Navya AS Department of Pharmacognosy, Government College of Pharmacy, Bengaluru,

More information

Research Article. Simultaneous spectrophotometric estimation of Paracetamol and Aceclofenac by second order derivative method in combined dosage form

Research Article. Simultaneous spectrophotometric estimation of Paracetamol and Aceclofenac by second order derivative method in combined dosage form Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(6):512-517 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Simultaneous spectrophotometric estimation of Paracetamol

More information

Available online on Journal of Advanced Drug Delivery 2014; 1(4); ISSN:

Available online on  Journal of Advanced Drug Delivery 2014; 1(4); ISSN: Available online on www.jadd.in Journal of Advanced Drug Delivery 2014; 1(4); 144-156 ISSN: 2348-3792 Research Article Development and Validation of Stability-Indicating HPTLC Method for Estimation of

More information

Lonicera japonica Flower Dry Extract. Proposed For Development Version 0.1. Published on Herbal Medicines Compendium (

Lonicera japonica Flower Dry Extract. Proposed For Development Version 0.1. Published on Herbal Medicines Compendium ( Published on Herbal Medicines Compendium (https://hmc.usp.org) Lonicera japonica Flower Dry Extract Proposed For Development Version 0.1 Lonicera japonica Flower Dry Extract DEFINITION The article is prepared

More information

International Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010

International Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010 International Journal of Pharmacy and Pharmaceutical Sciences Vol 2, Issue 1, 2010 RP HPLC ESTIMATION OF EZETIMIBE IN TABLET DOSAGE FORMS NAGARAJU. P *, KRISHNACHAITHANYA. K, CHANDRABABU. D, SRINIVAS.

More information

Development And Validation Of Rp-Hplc Method For Determination Of Velpatasvir In Bulk

Development And Validation Of Rp-Hplc Method For Determination Of Velpatasvir In Bulk International Journal of Engineering Science Invention (IJESI) ISSN (Online): 2319 6734, ISSN (Print): 2319 6726 www.ijesi.org PP. 36-41 Development And Validation Of Rp-Hplc Method For Determination Of

More information

Development and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage form

Development and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage form IJPAR Vol.5 Issue 4 Oct - Dec -2016 Journal Home page: ISSN:2320-2831 Research article Open Access Development and validation of septrophotometricmethods for the estimation of rasagiline in tablet doage

More information

STABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG AND IT S PHARMACEUTICAL DOSAGE FORM

STABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG AND IT S PHARMACEUTICAL DOSAGE FORM International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 3, Issue 3, 2011 Research Article STABILITY INDICATING RP HPLC METHOD FOR ANALYSIS OF DORZOLAMIDE HCl IN THE BULK DRUG

More information

A rapid identification of anthocyanins in various plants from Burkina Faso by HPTLC/MS

A rapid identification of anthocyanins in various plants from Burkina Faso by HPTLC/MS A rapid identification of anthocyanins in various plants from Burkina Faso by HPTLC/MS Presented by Rosella SPINA July 2-4, 2014 Lyon Background- Anthocyanidins chemistry A B Background- Anthocyanins >

More information

DEVELOPMENT AND VALIDATION OF AN HPTLC METHOD FOR DETERMINATION OF MINOCYCLINE IN HUMAN PLASMA

DEVELOPMENT AND VALIDATION OF AN HPTLC METHOD FOR DETERMINATION OF MINOCYCLINE IN HUMAN PLASMA ACTA CHROMATOGRAPHICA, NO. 19, 2007 DEVELOPMENT AND VALIDATION OF AN HPTLC METHOD FOR DETERMINATION OF MINOCYCLINE IN HUMAN PLASMA G. K. Jain *, N. Jain, Z. Iqbal, S. Talegaonkar, F. J. Ahmad, and R. K.

More information

Development and validation of RP-HPLC method for determination of marker in polyherbal marketed Kankasava formulations

Development and validation of RP-HPLC method for determination of marker in polyherbal marketed Kankasava formulations Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2011: 3 (5) 28-33 (http://scholarsresearchlibrary.com/archive.html) ISSN 0974-248X USA CODEN: DPLEB4

More information

Practical 1 Procedure Iron by Titrimetry

Practical 1 Procedure Iron by Titrimetry Practical 1 Procedure Iron by Titrimetry Introduction This experiment quantifies Fe 2+, ferrous iron, by reacting it with ceric sulphate in a 1:1 reaction, i.e. one mole of ferrous iron reacts with one

More information

Int. Res J Pharm. App Sci., 2012; 2(5): ISSN:

Int. Res J Pharm. App Sci., 2012; 2(5): ISSN: Research Article International Research Journal of Pharmaceutical and Applied Sciences (IRJPAS) Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(5):20-26 DEVELOPMENT AND VALIDATION

More information

Development and Validation of HPTLC Method for Estimation of Tenoxicam and its Formulations

Development and Validation of HPTLC Method for Estimation of Tenoxicam and its Formulations Research Paper Development and Validation of HPTLC Method for Estimation of Tenoxicam and its Formulations S. CHANDEL, C. R. BARHATE 1, A. R. SRIVASTAVA, S. R. KULKARNI 2 AND C. J. KAPADIA* Departments

More information

CYCLOSERINE Final text for addition to The International Pharmacopoeia. (November 2008) CYCLOSERINUM CYCLOSERINE

CYCLOSERINE Final text for addition to The International Pharmacopoeia. (November 2008) CYCLOSERINUM CYCLOSERINE December 2008 CYCLOSERINE Final text for addition to The International Pharmacopoeia (November 2008) This monograph was adopted at the Forty-third WHO Expert Committee on Specifications for Pharmaceutical

More information

DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR CLOPIDOGREL BISULFATE IN BULK AND FORMULATIONS

DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR CLOPIDOGREL BISULFATE IN BULK AND FORMULATIONS Int. J. Chem. Sci.: 10(1), 2012, 449-456 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR CLOPIDOGREL BISULFATE IN BULK AND FORMULATIONS PRAVIN CHOLKE

More information

Development and validation of HPTLC method for simultaneous estimation of canagliflozin and metformin hydrochloride in bulk and tablet dosage form

Development and validation of HPTLC method for simultaneous estimation of canagliflozin and metformin hydrochloride in bulk and tablet dosage form Development and validation of HPTLC method for simultaneous estimation of canagliflozin and metformin hydrochloride in bulk and tablet dosage form Bhole RP, Gandhi JS, Wankhede SB Department of Pharmaceutical

More information

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: , ISSN(Online): Vol.9, No.9, pp , 2016

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: , ISSN(Online): Vol.9, No.9, pp , 2016 International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304, ISSN(Online): 2455-9563 Vol.9, No.9, pp 189-199, 2016 Stability indicating thin-layer chromatographic determination of

More information

Method Development and Validation for the Estimation of Darunavir in Rat Plasma by RP-HPLC

Method Development and Validation for the Estimation of Darunavir in Rat Plasma by RP-HPLC World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original

More information

CHAPTER 5 PHYTOCHEMICAL STUDIES OF CYCLEA PELTATA

CHAPTER 5 PHYTOCHEMICAL STUDIES OF CYCLEA PELTATA CHAPTER 5 PHYTOCHEMICAL STUDIES OF CYCLEA PELTATA Aim: To investigate the phytochemical compounds of C. peltata extracts (Hexane, Chloroform, Methanol, Crude and Alkaloid extracts) 5.1. Introduction Plants

More information

DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 9 DETERMINATION OF DRUG RELEASE DURING DISSOLUTION OF NICORANDIL IN TABLET DOSAGE FORM BY USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 9 Determination of drug release during

More information