Chinese Journal of Applied Entomology 2014, 51(2): DOI: /j.issn 信息物质的化学分析技术 黄翠虹 , ; 2.
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1 Chinese Journal of Applied Entomology 204, 5(2): DOI: /j.issn 信息物质的化学分析技术 * 李静静 雷彩燕 黄翠虹 2 周琳 闫凤鸣 **., ; 2., 摘要 - GC-MS HPLC GC-MS 关键词 - Chemical analysis techniques for infochemicals LI Jing-Jing LEI Cai-Yan HUANG Cui-Hong 2 ZHOU Lin YAN Feng-Ming ** (. College of Plant Protection, Henan Agricultural University, Zhengzhou ,China; 2. Shunde Polytechnic College, Guangdong , China) Abstract Gas chromatography-mass spectrum (GC-MS) and high pressure liquid chromatography (HPLC) are the two techniques most frequently used in research on chemical ecology. Drawing on the authors experiences, this paper discusses key points related to sample preparation, the principles of GC-MS and HPLC, how to select techniques for chemical analysis, the qualification and quantification of samples, and describes and discusses some examples in detail. Key words chemical analysis, GC-MS, HPLC, sample preparation, qualification and quantification GC-MS HPLC 样品制备要点 20 * ** fmyan@henau.edu.cn
2 2 : mg mg 25 8~2 h 2 GC-MS 和 HPLC 原理 2. 气相色谱 - 质谱联用分析 (GC-MS) Gas chromatography GC 2.3 气相色谱柱的选择 Mass spectrum MS ~0-4 g - Gas chromatographymass spectrum GC-MS 2.2 高压液相色谱 (HPLC) High pressure liquid chromatography HPLC GC 3 DB-5 DB-7 DB-wax 2
3 588 Chinese Journal of Applied Entomology 5 30 m 0.25mm 3 分析方法的选择 3. 根据分析对象 挥发性植物次生物质 3.2 根据对化合物的了解程度 GC DIBMOA HPLC GC-MS 4 化学分析过程 4. 昆虫信息素 GC GC GC-EAD Zhang et al. 999 GC-EAD GC GC-MS GC GC Polarity 表 一些 GC 色谱柱的性质和可用于分析的化学物质类别 Table Properties and applications of some GC columns Applicable columns Examples of Non-polar DB-5 HP-5 EP-5 Medium-polar DB-70 OV-70 BP-0
4 2 : 589 Polar DB-Wax HP-20M MS 5.3 HPLC-MS 分析玉米中环氧肟酸类化合物 的含量 4.3 化合物的定量 00 5 应用实例 5. GC-MS 分析转 Bt 基因棉挥发性化学物质 GC-EAD GC-MS Finnigan Mat TSQ700 HP m 0.25 mm DB-Wax column J&W Scientific Folsom CA USA 50 5 min 8 /min HPLC 分析芥菜中芥子油苷成分和含量 30 mm 250 mm 4 mm C8RP Nucleosil 20-5 C8 5 μm Macherey and Nagel 20 μl 25% M TAS( ) (Tetrabutylammonium hydrogen sulfate), ml/min 25 min Varian 2050 HP nm 2000 (Aglient 00 series HPLC DAD detector Mass Spectrum) 20 μl Sepax HP-C8 column (250 mm 4.6 mm 5 μm) 30 ml/min 263 nm %TFA/ L H2O 2006 Huang et al HPLC 2 表 2 环氧肟酸类化合物 HPLC 分析洗脱梯度 * Table 2 Gradient elution for HPLC analysis of cyclic hydroxamic acids Time min B (% ) * A=H 2O 0.027%TFA B=MeOH. *Elutent system: A=H 2O with 0.027%TFA B=MeOH. 200 nm 400 nm DIMBOA HMBOA DIBOA 263 nm 3 参考文献 (References) Huang CH, Yan FM, Wang XY, Xue K, Wang RJ, Xu CR, Distribution and variation of cyclic hydroxamic acids in maize induced by the Asian corn borer, Ostrinia furnacalis. Z. Naturforsch., 6c (3/4):
5 590 Chinese Journal of Applied Entomology 5 Zhang QH, Birgersson G, Zhu J, Löfstedt C, Löfqvist J, Schlyter F, 999. Leaf volatiles from nonhost deciduous trees: variation by tree species, season and temperature, and electrophysiological activity in Ips typographus. J. Chem. Ecol., 25(8): [Huang CH, Studies on the Asian corn borer on feeding to induct of the secondary metabolites in Maize. Doctoral Dissertation.. Beijing: Peking University.] 2000, 43 (3): [Yan FM, Role of glucosinolates in the host plant part preferences by the cabbage aphid, brevicoryne brassicae. Acta Entomologica Sinica, 43(3): ], [Yan FM, 20. Chemical Ecology (Second Edition). Beijing :Science Press ] Bengtsson M, Witzgall P, Anderson P, Bt 45(4): [Yan FM, Xu CR, Bengtsson M, Witzgall P, Anderson P, Volatile compositions of transgenic Bt cotton and their electrophysiological effects on the cotton bollworm. Acta Entomologica Sinica, 45(4): ],,, : [Zhang QY, Li SW, Zhang HQ, 992. Modern biological experiment technology. Beijing: Beijing Normal University press ]
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