Supporting Information. Hexagonal Cobalt Oxyhydroxide-Carbon Dots Hybridized Surface: High Sensitive Fluorescence Turn-on Probe for Monitoring of
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1 Supporting Information Hexagonal Cobalt Oxyhydroxide-Carbon Dots Hybridized Surface: High Sensitive Fluorescence Turn-on Probe for Monitoring of Ascorbic Acid in Rat Brain Following Brain Ischemia Linbo Li,, Chao Wang, Kangyu Liu, Yuhan Wang, Kun Liu, Yuqing Lin*, Department of Chemistry, Capital Normal University, Beijing , China College of Resources Environment and Tourism, Capital Normal University, Beijing , China Capital University of Physical Education and Sports, Beijing , China The supporting information contains a description of the quantum yield measurements, further characterization and condition investigation data in support of the manuscript. Table S1. Quantum yield of the Tris-derived CDs. Table S2. Comparison of different methods for determination of ascorbic acid. Figure S1. EDX spectrum of Tris-derived CDs-CoOOH. Figure S2. (a) The stability of PL emission response of Tris-derived CDs over 15 days. Excitation at 365 nm. (b) Emission spectra of the Tris-derived CDs with excitation of different wavelength (from 320 nm to 520 nm). Figure S3. XRD patterns of the Tris-derived CDs and Tris-derived CDs-CoOOH. The measurement is conducted by using those powders. The red markers correspond to the (012) and (018) plane of Tris-derived CDs-CoOOH, respectively. Figure S4. UV-vis absorption of aqueous solutions of CoCl 2 (pink line) and CoOOH solution (red line). Figure S5. UV-vis absorption of aqueous solutions of CoOOH (dash line) and the PL emission spectrum of the Tris-derived CDs (solid line) with excitation of different wavelength (from 320 nm to 520 nm). Figure S6. Photograph of sedimentation condition of non-standardized Tris-derived CDs-CoOOH solution with time. Figure S7. The required time for reaction equilibrium between Tris-derived CDs-CoOOH probe and ascorbic acid (AA). Excitation at 365 nm. S1
2 Quantum yield (QY) measurements.the fluorescence quantum yield of the Tris-derived CDs was determined by comparing the integrated fluorescence intensities (excited at 365 nm) and the absorbance values (at 365 nm) of the Tris-derived CDs by using quinine sulfate (QY = 0.54 in deionized water) as the standard sample. Then use the equation (1): Q=Q r I E r n 2 I r E n2 r (1) Where Q is the quantum yield, I is the measured integrated emission intensity, n is the refractive index, and E is the optical density. The subscript r refers to the standard with known QY. To minimize the re-absorption effects, absorbencies in the 10 mm fluorescence cuvette were kept under 0.1 at the excitation wavelength (365 nm). Table S1. Quantum yield of the Tris-derived CDs Sample I E n Q Quinine sulfate Tris-derived CDs S2
3 Table S2. Comparison of different methods for determination of AA. Method Liquid chromatography Microfabricated Electrophoresis Chips Linear range (µm) Detection limit (µm) Application Comments Reference blood plasma and serum spiked bioassay Electrochemistry spiked bioassay On-line Electrochemical Detection rat striatum HPLC-ED Flow injection fluorometric determination fruits and human blood serum soft drink and fruit juice Fluorescent probe 5-50 / plasma of rat Fluorescent probe Fluorescent probe living cells and in vivo rat brain microdialysates multi-step, multi-step, rapid, simplicity in instrument and In vivo, continuous, fast multi-step, moderate convenient, simplicity in instrument and convenient, simplicity in instrument and convenient, simplicity in instrument and 5a 5b 5c 1a 5d 7a 7b 9a This work S3
4 Figure S1. EDX spectrum of Tris-derived CDs-CoOOH. (a) (b) Figure S2. (a) The stability of PL emission response of Tris-derived CDs over 15 days. Excitation at 365 nm. (b) Emission spectra of the Tris-derived CDs with excitation of different wavelength ( from 320 nm to 520 nm). S4
5 Figure S3. XRD patterns of the Tris-derived CDs and Tris-derived CDs-CoOOH. The measurement is conducted by using those powders. The red markers correspond to the (012) and (018) plane of Tris-derived CDs-CoOOH, respectively. Figure S4. UV-vis absorption of aqueous solutions of CoCl 2 (pink line) and CoOOH solution (red line). S5
6 Figure S5. UV-vis absorption of aqueous solutions of CoOOH (dash line) and the PL emission spectrum of the Tris-derived CDs (solid line) with excitation of different wavelength (from 320 nm to 520 nm). Figure S6. Photograph of sedimentation condition of non-standardized Tris-derived CDs-CoOOH solution with time. S6
7 Figure S7. The required time for reaction equilibrium between Tris-derived CDs-CoOOH probe and AA. Excitation at 365 nm. S7
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