DETERMINATION OF EPHEDRINE, PSEUDOEPHEDRINE, AND NOREPHEDRINE IN HUMAN URINE USING GC-MS OF THE METHYLBORONIC ACID DERIVATIVES
|
|
- Charla Horn
- 5 years ago
- Views:
Transcription
1 THE MANUSCRIPT FOR 2005 TIAFT PROCEEDINGS DETERMINATION OF EPHEDRINE, PSEUDOEPHEDRINE, AND NOREPHEDRINE IN HUMAN URINE USING GC-MS OF THE METHYLBORONIC ACID DERIVATIVES Jin Young Kim a, *, Moon Kyo In a, Bong Chul Chung b a Drug Analysis Laboratory, Supreme Prosecutors Office, Seoul , Korea b Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O.Box 131, Cheongryang, Seoul , Korea *Corresponding author. Tel: ; Fax: address: paxus@spo.go.kr
2 Abstract A method using methylboronic acid derivatization and gas chromatography-mass spectrometry (GC-MS) was developed for the determination of ephedrine, pseudoephedrine, and norephedrine in human urine. Urine samples containing a deuterated internal standard were extracted with diethyl ether under an alkaline condition. The extracts were evaporated, and then the residue was derivatized by the ring-formation reaction of ephedrines with methylboronic acid (MBA). The derivatized extract (1 μl) was injected into the GC-MS. A good separation by GC was only achieved after derivatization of the amine and hydroxy groups, which also increased sensitivity to a limit of detection (LOD) of 5.0 ng/ml for both ephedrine and pseudoephedrine and 20.0 ng/ml for norephedrine. The recoveries were in the range %. The responses were linear with correlation coefficients (r 2 > ) for all analytes. The method was successfully applied to the selective determination of ephedrines from biological background or interference in positive control samples. Keywords: Ephedrines; GC-MS; Methylboronic acid; Selectivity - 2 -
3 1. Introduction Ephedrines (ephedrine, pseudoephedrine, and norephedrine) are sympathomimetic amines with central nervous system stimulating properties [1,2]. These substances are ingredients of many medicines commonly used in the treatment of flu, rhinitis, colds, and allergies [3]. Ephedrines share a chemical structure similar to amphetamines. Their structures differ from that of methamphetamine only by the substitution of a hydroxyl group for the hydrogen on the alpha-carbon atom. Because of their structural similarity, the misidentification as methamphetamine or amphetamine by immunoassays may be caused by the presence of ephedrines in the urine specimens [4,5]. The TDx/TDxFLx Amphetamine/Methamphetamine Ⅱ Assay fluorescence-polarization immunoassay (FPIA) used as an initial test also displays the cross-reactivity with ephedrines and other phenylisopropylamine derivatives that may cause false-positive results [6,7]. Several methods have been reported for the qualification or quantification of ephedrines, including high-performance liquid chromatography (HPLC) [8-10], gas chromatography (GC) [11,12], and gas chromatography-mass spectrometry (GC-MS) [5,13-15]. Due to specificity and sensitivity, most of the procedures devoted to drug detection in biological matrices are based on GC-MS. However the determination of ephedrines is not easy, because of the similar retention time of their derivatives in the gas chromatograph, as well as a similar mass fragmentation pattern. Therefore, a fast and reliable method is needed for the simultaneous quantification and confirmation of ephedrines. In this study, a selective, rapid, accurate GC-MS method with derivatization (O,Nmethylborated derivatives) was developed to confirm and quantify ephedrines in human - 3 -
4 urine. It is important to emphasize the following: (a) only one cyclic derivative was employed to form all the ephedrines; and (b) both a quadrupole (MS) and an ion-trap mass (MS-MS) spectrometer were applied to analyze the urine samples. 2. Experimental procedures 2.1. Chemicals Ephedrine, pseudoephedrine, and norephedrine were purchased from Cerilliant (Austin, TX, USA) in vials at a concentration of 1.0 mg/ml in methanol. Ephedrine-d 3 as their deuterated internal standard (IS) was purchased from Aldrich (Milwaukee, WI, USA). Diethyl ether and ethyl acetate were purchased from J. T. Baker (Phillipsburg, NJ, USA). All of the solvents were of HPLC grade. The derivatizing agent, methylboronic acid (MBA), was purchased from Fluka (Buchs, Switzerland) Preparation of solutions Working standard solutions (1 and 10 μg/ml) of ephedrine, pseudoephedrine, and norephedrine were prepared by appropriate dilution with methanol. A stock solution of ephedrine-d 3 was prepared by accurately weighing ~10 mg and dissolving in 10 ml. Subsequently, ephedrine-d 3 solutions were diluted with concentrations of 1, 10, and 100 μg/ml. All of these solutions were stored at -20 C in the absence of light until required Sample preparation - 4 -
5 Urine (1 ml) was submitted to a liquid liquid extraction 5ml of diethyl ether (twice), after adding 50 μl of methanolic solution of ephedrine-d 3 10 μg/ml internal standard solution, 1.0 ml of distilled water, and 0.2 ml of NaOH solution (2 M). Samples were mixed on a shaker for 15 min and centrifuged for 5 min (2800 g). The organic phase was transferred to a test tube ( mm) and evaporated until dry under a fine stream of nitrogen at 45 C. To the residue, a 50 μl mixture of MBA in ethyl acetate (1.0 mg/ml) was added and heated at 70 C for 15 min in a dry heating block. A 1 μl aliquot was injected into the GC-MS system Gas chromatography-mass spectrometry In the quantification study, the GC-MS system consisting of an Agilent Technologies (Palo Alto, CA, USA) 6890N GC coupled to a 5973 Network mass-selective detector (MSD) was used. The system was controlled by Drug Analysis Chemstation G1701CA software (C.00.00, Agilent Technologies). The gas chromatograph was equipped with a capillary column (DB-5MS, 25 m 0.25 mm i.d., 0.25 μm film thickness, J&W scientific, Folsom, CA, USA). Helium was used as the carrier gas at a flow rate of 0.8 ml/min. The GC temperature program was as follows: initial temperature of 120 C, was held for 1 min, increased to 255 C at a rate of 15 C/min, then to 290 C at a rate of 35 C/min, and then held for 1 min. The split ratio was 1:6, the injection temperature 250 C, and transfer line temperature was 280 C. The mass spectrometer was operated at 70 ev in the electron impact mode with selected ion monitoring (SIM) for the quantification. The selected ion groups of the derivatized analytes were monitored as follow: m/z 174, 189, and 132 (ephedrine and pseudoephedrine); m/z 160, 175, and 118 (norephedrine); and m/z 177 (ephedrine-d 3 )
6 The conditions of electron impact (EI) ionization and tandem mass spectrometer (MS-MS) with a ThermoFinnigan (San Jose, CA, USA) TRACE GC 2000 coupled to a ThermoFinnigan Polaris Q MS was employed to confirm the results. The gas chromatograph was equipped with a capillary column (DB-5MS, 25 m 0.25 mm i.d., 0.25 μm film thickness, J&W scientific, Folsom, CA, USA). The same GC temperature program above-noted was applied, and a split-injection mode with the split ratio (1:10) was used. The conditions for the MS-MS product ion mode applied are summarized in Table Validation of the analytical method The linearity of the method was checked in the concentration range ng/ml for ephedrine and pseudoephedrine and ng/ml for norephedrine. Using 1.0 ml samples of human urine spiked with methanolic solutions of ephedrine, pseudoephedrine, and norephedrine, a six-point calibration curve was established. The linear regression analysis was performed on the peak area ratios of analyte to the internal standard versus the analyte concentrations. For inter-day and intra-day validation, precision and accuracy were calculated for three concentrations corresponding to quality control (QC) samples of the method. To verify the method limit-of-detection (LOD) the signal-to-noise (S/N) ratio of the quantification ions was greater than the S/N ratio 3:1. Extraction recoveries of each analyte were quantified at three different concentrations (200, 500, and 1000 ng/ml) in five replicates each. 3. Results and discussion - 6 -
7 3.1. Chromatography and mass spectrometry Ephedrine, pseudoephedrine, and norephedrine were separated using a DB-5MS capillary column and a temperature-programmed GC. The methylborated ephedrines were identified by comparing the retention time with reference standards (Fig. 1). The SIM chromatograms for these drugs clearly indicated the order of elution. The methylborated norephedrine, pseudoephedrine, and ephedrine appear to have sharp and well-defined peaks at retention time of 4.50, 4.80, and 4.84 min, respectively. EI mass spectra obtained for the derivatives of (a) ephedrine and pseudoephedrine, (b) norephedrine, and (c) ephedrine-d 3 are shown in Fig. 2. The molecular ions, m/z 189 for ephedrine and pseudoephedrine and m/z 175 for norephedrine, were formed abundantly and also used as qualification ions Evaluation of validation data The described method was validated by evaluating selectivity, linearity, precision and accuracy, LOD, and recovery. Ephedrine, pseudoephedrine, and norephedrine are welldefined chromatographically after derivatization. Comparison of GC-MS SIM chromatograms for the blank urine sample and for a urine sample spiked with analyte and internal standard, no chemical interferences were observed from the biological matrix (Fig. 3). Table 2 presents the summarized quantitative parameters. Calibration standards at six concentration levels for each analyte were used to construct the calibration curves. Correlation coefficients were between and , indicating good linear regression. The limit of detection (LOD) was defined as the signal-to-noise ratio of 3 for - 7 -
8 each compound. The extraction recoveries of ephedrine, pseudoephedrine, and norephedrine were determined at three concentration levels in replicates of five. The peak-area ratios for each analyte were compared between those for the samples that had been spiked with analytes prior to extraction and samples to which the sample levels of analytes were added after extraction. The recoveries of the analytes were %. Table 3 presents the intra- and inter-day precision and accuracy of the method. Intraday precision and accuracy were obtained by analyzing five replicates using three different spiked-urine samples (200, 500, and 1000 ng/ml) of ephedrine, pseudoephedrine, and norephedrine added with 500 ng of ephedrine-d 3. For intra-day assays, the precision ranged from 0.9 to 8.6% for all analytes. To determine the precision, the coefficients of variations (% C.V.) were calculated for the replicate measurements. To obtain the inter-day precision and accuracy, the same spiked humanurine samples were analyzed on each of 3 days for human urine. For inter-day assays, the overall precision ranged from 1.6 to 13.7% for all analytes. Considering the complexity of the biological matrix, these results were regarded as satisfactory Separation of ephedrines from compounds that have a similar chemical structure The fluorescence-polarization immunoassay as a screening test was used for the rapid determination of amphetamine type stimulants in urine samples. The fluorescencepolarization immunoassay method displays cross-reactivities with ephedrines and phenylisopropylamine derivatives (methoxyphenamine, mexiletine, methylephedrine, phenmetrazine, phendimetrazine) that may cause false-positive results. False-positive cases have been reviewed from the urine samples of drug abusers in our laboratory. Among these cases, ephedrines cross-reacted most frequently with the immunoassay
9 Thus the described method was applied for the selective determination of ephedrines to the methanolic solution of eight drugs containing norephedrine, ephedrine, pseudoephedrine, methoxyphenamine, mexiletine, methylephedrine, phenmetrazine, and phendimetrazine. The methylborated derivatives of ephedrines demonstrated the followings: (a) a better separation due to less peak-tailing, especially in the case of norephedrine; (b) a shift of retention time in the case of ephedrine, pseudoephedrine, and norephedrine; and (c) a clear separation of methoxyphenamine. Fig. 4 shows representative GC-MS total ion chromatograms of before- and after-derivatization with MBA in ethyl acetate Application to urine of a ephedrine user The method was applied to analysis of a positive control urine sample obtained from a ephedrines user. Fig. 3 shows the SIM-chromatogram for detecting ephedrine, pseudoephedrine, and norephedrine in this case. The concentrations measured in the urine sample are 3040 ng/ml (ephedrine), 8800 ng/ml (pseudoephedrine), and 405 ng/ml (norephedrine]. When analyzing the MS-MS (ion trap) of the parent ion in the product ion-scan mode, one can effectively check for the presence of the above-mentioned analytes found in the GC-MS-MS chromatogram. 4. Conclusions The mass spectral identification of ephedrines using methylboronic acid in ethyl acetate as a derivatizing mixture has many advantages. It is a convenient, quick, - 9 -
10 selective, and unmistakable way to confirm ephedrines in human urine. Methylboronate derivatives can clearly separate ephedrines from adjacent and other sympathomimetic amines on the GC chromatogram using cyclic derivatives. The method has been validated and effectively applied to urine sample collected from ephedrine user
11 References [1] P.J. van der Merwe, S.E. Hendrikz, J. Chromatogr. B. 663 (1995) 160. [2] J. Dickerson, D. Perrier, M. Mayersohn, R. Bressler, Eur. J. Clin. Pharmacol. 14 (1978) 253. [3] G. Joseph, J. Chromatogr. 307 (1984) 220. [4] C.L. Hornbeck, J.E. Carrig, R.J. Czarny, J. Anal. Toxicol. 17 (1993) 257. [5] A. Dasgupta, C. Gardner, J. Forensic Sci. 40 (1995) [6] J. D'Nicuola, R. Jones, B. Levine, M. L. Smith, J. Anal. Toxicol. 16 (1992) 211. [7] Amphetamine/Methamphetamine Ⅱ. In: List No. 1A99, /R2, Abbott Park, IL: Abbott Laboratories, 2001, p. 1. [8] D. J. Hood, H. Y. Cheung, J. Pharm. Biomed. Anal. 30 (2003) [9] G. Gmeiner, T. Geisendorfer, J. Kainzbauer, M. Nikolajevic, H. Tausch, J. Chromatogr. B. 768 (2002) 215. [10] Y. Makino, Y. Urano, T. Nagano, J. Chromatogr. A. 947 (2002) 151. [11] P. Van Eenoo, F. T. Delbeke, K. Roels, P. De Backer, J. Chromatogr. B. 760 (2001) 255. [12] J. Jonsson, R. Kronstrand, M. Hatanpaa, J. Forensic Sci. 41 (1996) 148. [13] M. E. Spyridaki, C. J. Tsitsimpikou, P. A. Siskos, C. G. Georgakopoulos, J. Chromatogr. B. 758 (2001) 311. [14] G. Aymard, B. Labarthe, D. Warot, I. Berlin, B. Diquet, J. Chromatogr. B. 744 (2000) 25. [15] B. M. El-Haj, A. M. Al-Amri, M. H. Hassan, H. S. Ali, R. K. Bin Khadem, Forensic Sci. Int. 135 (2003)
12 Table 1 Mass spectrometric conditions of the GC-MS-MS method Source temperature ( C) 150 Start time (min) 4.0 Scan mode MS-MS; product ion mode Precursor ion (m/z) 174 (ephedrine and pseudoephedrine) 160 (norephedrine) 177 (ephedrine-d 3 ) Width (m/z) 1 Excitation voltage 1 Product ions (m/z) (ephedrine and pseudoephedrine) (norephedrine) (ephedrine-d 3 ) - 12-
13 Table 2 Validation data for the analysis of ephedrines in human urine Ephedrine Pseudoephedrine Norephedrine Concentration range (ng/ml) Linearity a LOD b (ng/ml) Recovery (% C.V., n = 5) 200 ng/ml 87.7 ± ± ± ng/ml 92.3 ± ± ± ng/ml 95.4 ± ± ± 7.2 a Linearity is described by the correlation coefficients for the calibration curve b Limit of detection (LOD) was defined as the concentration at which the characteristic ion was detectable on the corresponding mass chromatogram at signal-to-noise (S/N) =3 or greater - 13-
14 Table 3 Precision a and accuracy b for determining ephedrine and related compounds Drug Added amount (ng/ml) Mean (ng/ml) Intra-day (n=3) Accuracy (% bias) Precision (% C.V.) Mean (ng/ml) Inter-day (n=3) Accuracy (% bias) Precision (% C.V.) Epedrine Pseudoephedrine Norephedrine a Expressed as coefficient of variance of the peak area ratios of analyte/internal standard. b Calculated as [(mean calculated concentration nominal concentration)/nominal concentration]
15 Figure legends Fig. 1. Chemical structure of ephedrines: Ephedrine (A), pseudoephedrine (B), and norephedrine (C). Fig. 2. EI spectra of ephedrines derivatized with methaneboronate: Ephedrine and pseudoephedrine (A), norephedrine (B), and ephedrine-d 3 (C). Fig. 3. SIM GC-MS determination of ephedrines in human urine: Extract from blank urine (A) and Extract from amphetamine/methamphetamine false-positive human urine (B). Fig. 4. SIM GC-MS chromatograms of eight compounds (norephedrine, ephedrine, pseudoephedrine, methoxyphenamine, mexiletine, methylephedrine, phenmetrazine, and phendimetrazine) before (A) and after derivatization with MBA in ethyl acetate (B). The acquisition was made by using 50 μl of a standard mixture (100 ppm) containing eight compounds in Full-Scan mode (m/z ). The arrow in the panel (B) indicates norephedrine elutes before the derivatization
16 H OH H CH OH CH N CH CH N CH OH CH NH 2 A B C Fig
17 Abundance (A) 95 O B 90 N m/z (B) O B 95 NH m/z (C) O B N CD m/z Fig
18 (A) 1 Norephedrine 2 Pseudoephedrine 3 Ephedrine IS Relative Abundance m/z 174 m/z IS 30 Relative Abundance (B) & IS NL: m/z Relative Abundance m/z 177 IS Time (min) Time (min) Fig
19 (A) (a) 1 Norephedrine 2,3,4 2 Ephedrine Pseudoephedrine 4 Methoxyphenamine Mexiletine 6 Methylephedrine 7 Phenmetrazine 8 Phendimetrazine Abundance (B) (b) 1 2, Methylborated norephedrine 2 Methylborated pseudoephedrine 3 Methylborated ephedrine 4 Methoxyphenamine 5 Mexiletine 6 Methylephedrine 7 Phenmetrazine 8 Phendimetrazine Time (min) Fig
[application note] INTRODUCTION EXPERIMENTAL. Specimens. Extraction
Testing for GHB in hair by GC tandem quadrupole MS Marie Bresson, Vincent Cirimele, Pascal Kintz, Marion Villain; Laboratoire Chemtox, Illkirch, France Timothy Jenkins, Waters Corporation, Manchester,
More informationQuantitative analysis of mitragynine in human urine by high performance liquid chromatography-tandem mass spectrometry
Quantitative analysis of mitragynine in human urine by high performance liquid chromatography-tandem mass spectrometry Shijun Lua, Buu N. Trana, Jamie L. Nelsenb, Kenneth M. Aldousa. Journal of Chromatography
More informationApplication Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors
Trace-Level Analysis of Melamine in Milk Products on Agilent 789A/5975C GC/MSD Using a ew Agilent J&W DB-5ms Ultra Inert Column and SampliQ SCX Cartridges Application ote Gas Chromatography/Mass Spectrometry/Food
More informationCharacteristic of Calibration Curve Resulting from the Use of 2 H-analogs of the Analyte as Internal Standards--Methamphetamine Example
FORENSIC SCIENCE JOURNAL SINCE 00 Forensic Science Journal 004;3:59-70 Characteristic of Calibration Curve Resulting from the Use of H-analogs of the Analyte as Internal Standards--Methamphetamine Example
More informationRapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry
Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Application Note Food Authors Jianqiu Mi, Zhengxiang Zhang, Zhixu Zhang,
More informationAnalyst
PAPER www.rsc.org/analyst Analyst Detector response and intensity cross-contribution as contributing factors to the observed non-linear calibration curves in mass spectrometric analysis Meng-Jie Sie, a
More informationDetermination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry
Determination of Beta-Blockers in Urine Using Supercritical Fluid Chromatography and Mass Spectrometry Application Note Doping Control Authors Prof. Maria Kristina Parr Freie Universität Berlin Institute
More informationAmphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS
Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 643 LC/MS/MS Application Note Forensic Toxicology Authors Jason Hudson, Ph.D., James Hutchings, Ph.D., and Rebecca Wagner,
More informationDetermination of Cannabinoids (THC) in Biological Samples
Determination of Cannabinoids (THC) in Biological Samples Application Note Forensic Toxicology Authors Joe Crifasi Saint Louis University Forensic Toxicology Laboratory Saint Louis, MO, USA Ron Honnold
More informationTrace analysis of mesityl oxide and diacetone alcohol in pharmaceuticals by capillary gas chromatography with flame ionization detection
Trade Science Inc. September 2009 Volume 8 Issue 3 ACAIJ, 8(3) 2009 [346-349] Trace analysis of mesityl oxide and diacetone alcohol in pharmaceuticals by capillary gas chromatography with flame ionization
More informationPlasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS
Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SimpliQ WCX SPE, 129 Infi nity LC, and 646 Triple Quadrupole LC/MS Application Note Clinical Research Authors Linda Côté and Christophe
More informationDetermination of Elemental Sulfur in Explosives and. Explosive Residues by Gas Chromatography-Mass Spectrometry. Kimberly S.
Determination of Elemental Sulfur in Explosives and Explosive Residues by Gas Chromatography-Mass Spectrometry Kimberly S. Bradley Illinois State Police, Springfield Forensic Science Laboratory 24 Hill
More informationLC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode
LC/MS/MS qua ntitation of β-estradiol 17-acetate using an Agilent 6460 Triple Quadrupole LC/MS working in ESI negative ion mode Application Note Authors Siji Joseph Agilent Technologies India Pvt. Ltd.
More informationVALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION
VALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION Andrew J. Aubin and Tanya L. Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION Benzocaine (4-Aminobenzoic
More informationAnalysis of Biomarkers in Crude Oil Using the Agilent 7200 GC/Q-TOF
Analysis of Biomarkers in Crude Oil Using the Agilent 7 GC/Q-TOF Application Note Petrochemical and Environmental Authors Frank David Research Institute for Chromatography, Kennedypark 6, B-85 Kortrijk,
More informationWADA Technical Document TD2003IDCR
IDENTIFICATION CRITERIA FOR QUALITATIVE ASSAYS INCORPORATING CHROMATOGRAPHY AND MASS SPECTROMETRY The appropriate analytical characteristics must be documented for a particular assay. The Laboratory must
More informationAnalysis of Cannabinoids and Amphetamines in Serum by RRLC/Triple Quadrupole Mass Spectrometry Using a Multimode Ion Source. Application.
Analysis of Cannabinoids and Amphetamines in Serum by RRLC/Triple Quadrupole Mass Spectrometry Using a Multimode Ion Source Application Toxicology Authors Jürgen Wendt Agilent Technologies Sales and Support
More informationSTANDARD OPERATING PROCEDURES SOP: 1828 PAGE: 1 of 14 REV: 0.0 DATE: 05/12/95 ANALYSIS OF METHYL PARATHION IN CARPET SAMPLES BY GC/MS
PAGE: 1 of 14 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY CONTENTS 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS
More informationAppendix II- Bioanalytical Method Development and Validation
A2. Bioanalytical method development 1. Optimization of chromatographic conditions Method development and optimization of chromatographic parameters is of utmost important for validating a method in biological
More informationSimultaneous Estimation of Residual Solvents (Isopropyl Alcohol and Dichloromethane) in Dosage Form by GC-HS-FID
Asian Journal of Chemistry Vol. 21, No. 3 (2009), 1739-1746 Simultaneous Estimation of Residual Solvents (Isopropyl Alcohol and Dichloromethane) in Dosage Form by GC-HS-FID PRAVEEN KUMAR BALIYAN*, R.P.
More informationBenzoylecgonine in Urine by SAMHSA GC/MS
application Note Gas Chromatography/ Mass Spectrometry Author Timothy D. Ruppel PerkinElmer, Inc. Shelton, CT 06484 USA Benzoylecgonine in Urine by SAMHSA GC/MS Introduction The United States Department
More informationA Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies
A Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies APPLICATION BENEFITS Regulatory guidelines and recommendations place a greater emphasis on the detection
More informationUltrafast Analysis of Methadone and Metabolite EDDP in Urine by the Agilent RapidFire High-Throughput Mass Spectrometry System
Ultrafast Analysis of and Metabolite in Urine by the Agilent RapidFire High-Throughput Mass Spectrometry System Application Note Forensic Toxicology Authors Mohamed Youssef and Vaughn P. Miller Agilent
More informationEPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS
EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS Christopher Borton AB SCIEX Golden, Colorado verview Described here is the analysis of
More information2,5-DIMETHOXY-4-IODOPHENETHYLAMINE Latest Revision: August 22, 2005
2,5-DIMETHOXY-4-IODOPHENETHYLAMINE Latest Revision: August 22, 2005 1. SYNONYMS CFR: CAS #: Other Names: Not Available Base: Not Available Hydrochloride: 69587-11-7 4-Iodo-2,5-dimethoxyphenethylamine 4-Iodo-2,5-dimethoxybenzeneethanamine
More informationEPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS
Application Note EPA Method 535 EPA Method 535: Detection of Degradates of Chloroacetanilides and other Acetamide Herbicides in Water by LC/MS/MS API 3200 LC/MS/MS System Overview Described here is the
More informationTechnical Procedure for Concentration Determination of Methamphetamine in Liquids via HPLC
Technical Procedure for Concentration Determination of 1.0 Purpose This procedure specifies the required elements for the preparation and use of the Agilent 1100/1200 series High Performance Liquid Chromatograph
More informationSeparation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers
Application Note Forensic and Toxicology - Criminalistics Separation of Enantiomers of Amphetamine-Related Drugs and Their Structural Isomers Using the Agilent 126 Infinity II SFC and Detection by Coupled
More informationDEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR IN-VIVO STUDY OF DICLOFENAC POTASSIUM
IJPSR (2013), Vol. 4, Issue 2 (Research Article) Received on 28 September, 2012; received in revised form, 24 November, 2012; accepted, 23 January, 2013 DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR
More informationSelective Formation of Benzo[c]cinnoline by Photocatalytic Reduction of 2,2 Dinitrobiphenyl with TiO 2 and UV light irradiation
Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2015 Content: Selective Formation of Benzo[c]cinnoline by Photocatalytic Reduction of
More informationSulfotepp impurities in Chlorpyrifos EC formulations
Page 1 of 16 Method DAS-AM-01-058 Sulfotepp impurities in Chlorpyrifos EC formulations A. ABSTRACT Method DAS-AM-01-058 has been validated for the analysis of the relevant impurity sulfotepp in chlorpyrifos
More informationPsychoactive Drugs Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 18 Psychoactive Drugs Urine LC-MS/MS Analysis Kit User Manual ZV-2001-0200-20 200 2-8 C Page 2 / 18 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE...
More informationGAFTI Analytical method for ISO/TS 16179:2012 Detection and Determination of Organotin Compounds in Footwear and Apparel Materials by GC-MS
GAFTI Analytical method for ISO/TS 16179:2012 Detection and Determination of Organotin Compounds in Footwear and Apparel Materials by GC-MS 1. Scope This method is applicable for determining the presence
More informationTomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories
APPLICATION NOTE 65200 Tomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories Authors Neloni Wijeratne, Claudia Martins, Mary
More informationLC-MS/MS Method for the Determination of Diclofenac in Human Plasma
LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core
More informationAnalytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS
Application Note Clinical Research Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Authors Yanan Yang 1, Victor Mandragon 2, and Peter Stone 1 1
More informationMethod - Determination of aromatic amines in mainstream cigarette smoke
British American Tobacco Group Research & Development Method - Determination of aromatic amines in mainstream cigarette smoke 1 SCOPE OF APPLICATION The method is applicaple to quantitative determination
More informationHigh-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes
High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes Joe DiBussolo, Marta Kozak Thermo Fisher Scientific, San Jose, CA Application
More informationUltrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System
Ultrafast Analysis of Buprenorphine and Norbuprenorphine in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System Application Note Authors Mohamed Youssef and Vaughn P. Miller Agilent
More informationAchieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer
APPLICATION NOTE 72385 Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer Authors Stephan Meding, Katherine Lovejoy, Martin Ruehl Thermo Fisher Scientific,
More informationMaximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System
Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Application Note Authors Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon, and
More informationYun W. Alelyunas, Mark D. Wrona, Russell J. Mortishire-Smith, Nick Tomczyk, and Paul D. Rainville Waters Corporation, Milford, MA, USA INTRODUCTION
Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma Yun W. Alelyunas, Mark
More informationMacrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS
Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS Application Note Food Testing and Agriculture Author Chen-Hao (Andy) Zhai and Rong-jie Fu Agilent Technologies (Shanghai)
More informationUncontrolled Copy. SOP 109 Ethylene Glycol Screen by Gas Chromatography/Mass Spectrometry. Table of Contents. 1. Principle of Assay...
Table of Contents 1. Principle of Assay... 3 2. Specimens... 3 3. Reagents and Materials (HPLC grade)... 3 4. Standards, Controls, and Solutions... 4 5. Equipment and Special Supplies... 5 6. Instrumentation...
More informationQuantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 1260 Infinity Binary LC system with UV detection
Quantification of growth promoters olaquindox and carbadox in animal feedstuff with the Agilent 126 Infinity Binary LC system with UV detection Application Note Food Author Srividya Kailasam Agilent Technologies,
More informationISSN: ; CODEN ECJHAO E-Journal of Chemistry , 7(2),
ISSN: 0973-4945; CODEN ECJHAO E-Journal of Chemistry http://www.e-journals.net 2010, 7(2), 629-635 Low-level Determination of Residual Methyl Methane Sulfonate and Ethyl Methane Sulfonate in Pharmaceuticals
More informationSTANDARD OPERATING PROCEDURES SOP: 1826 PAGE: 1 of 18 REV: 0.0 DATE: 03/30/95 ANALYSIS OF METHYL PARATHION IN WIPE SAMPLES BY GC/MS
PAGE: 1 of 18 CONTENTS 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS
More informationDetection of Phencyclidine in Human Oral Fluid Using Solid Phase Extraction and Liquid Chromatography with Tandem Mass Spectrometric Detection
Detection of Phencyclidine in Human Oral Fluid Using Solid Phase Extraction and Liquid Chromatography with Tandem Mass Spectrometric Detection Application Note Forensic Toxicology Authors Christine Moore,
More informationTechnical Procedure for Blood Cannabinoid Liquid-Liquid Extraction (BCLLE) for Analysis by LC-MS/MS
Technical Procedure for Blood Cannabinoid Liquid-Liquid Extraction (BCLLE) for Analysis by LC-MS/MS 1.0 Purpose - This procedure specifies the required elements for the extraction and quantitation of THC,
More informationDetection of 9-tetrahydrocannabinol ( 9-THC) in human urine by Solid Phase Extraction and HPLC.
Detection of 9-tetrahydrocannabinol ( 9-THC) in human urine by Solid Phase Extraction and HPLC. Abstract Chetna Mittal, PhD, Asha Oroskar, PhD,, Anil Oroskar, PhD Orochem Technologies Inc. Lombard, IL,
More informationMETHADONE and EDDP in urine by GC-MS Code GC Method of Confirmation by GC-MS
METHADONE and in urine by GC-MS Code GC48010 Method of Confirmation by GC-MS INTRODUCTION is a synthetic opioid used as a drug of choice in the detoxification of heroin (and / or opiates). is metabolized
More informationA Rapid Differentiation of Pseudoephedrine and Ephedrine by Stereospecific Derivatization
A Rapid Differentiation of Pseudoephedrine and Ephedrine by Stereospecific Derivatization Heather M. Robers 1, Crystal Hoffmann 1, Molly Ross 1, Karen Schmidt 1, Adrian Fuchs 1, Charles R. Cornett, Ph.D.
More informationMethod of determination of phtalates in spirituous beverages by gaschromatography/mass
OIV-MA-BS-33 Method of determination of phtalates in spirituous beverages by gas-chromatography/mass Type IV method 1. SCOPE OF APPLICATION This method applies to the detection and assay of some phthalates
More informationAnalysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF
application Note Liquid Chromatography/ Mass Spectrometry Author Sharanya Reddy PerkinElmer, Inc. Shelton, CT USA Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC-TOF
More informationAB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching Scores by Removal of Isobaric Interferences
AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching s by Removal of Isobaric Interferences Differential Mobility Used as a Tool to Address Selectivity Challenges Adrian M. Taylor
More informationRapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS
Rapid method development to study plasma stability of diverse pharmaceutical compounds using Rapid Resolution LC and triple quadrupole MS Application Note Drug Discovery Authors Srividya Kailasam Agilent
More informationRapid, Robust, and Sensitive Detection of 11-nor-D 9 - Tetrahydrocannabinol-9-Carboxylic Acid in Hair
Rapid, Robust, and Sensitive Detection of -nor-d 9 - Tetrahydrocannabinol-9-Carboxylic Acid in Hair Application Note Forensic Toxicology/Doping Control Authors David Engelhart Omega Labs, Inc. Mogadore,
More informationSupporting Information
1 Supporting Information 2 3 Discovery and implications of C 2 and C 3 perfluoroalkyl sulfonates in aqueous film forming foams (AFFF) and groundwater 4 Krista A. Barzen-Hanson a and Jennifer A. Field b*
More informationSIMULTANEOUS ESTIMATION OF CILOSTAZOL AND ASPIRIN IN SYNTHETIC MIXTURE USING HPTLC METHOD
Int. J. Chem. Sci.: 6(3), 2008, 1377-1384 SIMULTANEOUS ESTIMATION OF CILOSTAZOL AND ASPIRIN IN SYNTHETIC MIXTURE USING HPTLC METHOD JAYESH V. PATEL, C. N. PATEL, P. U. PATEL a PANKAJ H. PRAJAPATI, I. S.
More informationToxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative. Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish.
1 Supporting Information 2 3 Toxicity, Teratogenic and Estrogenic Effects of Bisphenol A and its Alternative Replacements Bisphenol S, Bisphenol F and Bisphenol AF in Zebrafish. 4 5 John Moreman, Okhyun
More informationAgilent 1200 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity
Agilent 12 Infinity Series HDR DAD Impurity Analyzer System for the Quantification of Trace Level of Genotoxic Impurity A case study with degraded omeprazole drug product Application Note Small Molecule
More information5-METHOXY-N,N-DIISOPROPYLTRYPTAMINE Latest Revision: June 20, 2005
5-METHOXY-N,N-DIISOPROPYLTRYPTAMINE Latest Revision: June 20, 2005 CH 3 O N CH(CH 3 ) 2 CH(CH 3 ) 2 N 1. SYNONYMS H CFR: CAS #: Other Names: Not Listed None 3-[2-(Diisopropylamino)ethyl]-5-methoxyindole
More informationRoger Bardsley, Applications Chemist; Teledyne Tekmar Page 1
Application Note Meeting the Korean Method ES 04602.1b for Vinyl Chloride, Acrylonitrile, and Bromoform ( 염화비닐, 아크릴로니트릴, 브로모포름 ) with Static and Dynamic Headspace GC/MS Roger Bardsley, Applications Chemist;
More informationAbstract. Experimental Sample Preparation
Determination of Benzoylecgonine in Human Urine Using Automated, In-Line, ITSP Solid Phase Extraction and Liquid Chromatography Mass Spectrometric Detection Thurman L. Allsup 1, Kenneth C. Lewis 1 and
More informationU.S. EPA Method 8270 for multicomponent analyte determination
ENVIRONMENTAL application note U.S. EPA Method 8270 for multicomponent analyte determination Elaine A. LeMoine and Herman Hoberecht Introduction Multicomponent analytes are compounds that yield several
More informationNORBUPRENORPHINE (Buprenorphine s Metabolite ) BUPRENORPHINE in urine by GC/MS Code GC Method of Confirmation by GC-MS
NORBUPRENORPHINE (Buprenorphine s Metabolite ) BUPRENORPHINE in urine by GC/MS Code GC44010 Method of Confirmation by GC-MS INTRODUCTION Buprenorphine is an analgesic with a long-time action, 25 to 40
More informationAutomated and accurate component detection using reference mass spectra
TECHNICAL NOTE 72703 Automated and accurate component detection using reference mass spectra Authors Barbara van Cann 1 and Amit Gujar 2 1 Thermo Fisher Scientific, Breda, NL 2 Thermo Fisher Scientific,
More informationAnalysis of Illegal Dyes in Food Matrices using Automated Online Sample Preparation with LC/MS
Application Note: 56 Analysis of Illegal Dyes in Food Matrices using Automated Online Sample Preparation with LC/MS Yang Shi, Catherine Lafontaine, Matthew Berube, John Fink, François Espourteille Thermo
More informationAnalysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS. No. GCMS No. SSI-GCMS-1401
Gas Chromatograph Mass Spectrometer No. GCMS-1401 Analysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS Introduction Development of methods for analysis of drugs of abuse has become a high
More informationDevelopment and validation a RP-HPLC method: Application for the quantitative determination of quetiapine fumarate from marketed bulk tablets
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(1):142-146 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and validation a RP-HPLC method: Application
More informationAssay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer
38 Current Trends in Mass Spectrometry November 6 Assay Robustness Improvement for Drug Urinalysis Using FAIMS and H-SRM on a Triple- Quadrupole Mass Spectrometer This article demonstrates the improved
More informationOpiates in Urine by SAMHSA GC/MS
application Note Gas Chromatography/ Mass Spectrometry Author Timothy D. Ruppel PerkinElmer, Inc. Shelton, CT 06484 USA Opiates in Urine by SAMHSA GC/MS Introduction The United States Department of Health
More informationMulti-residue GC-MS analysis. Richard Fussell CSL York, UK
Multi-residue GC-MS analysis Richard Fussell CSL York, UK e-mail: r.fussell@csl.gov.uk Outline of Presentation Background Gas Chromatography Mass Spectrometry - Options Capabilities and Applications Summary
More informationJWH-018 and JWH-022 as Combustion Products of AM2201
JWH-018 and JWH-022 as Combustion Products of AM2201 The emergence of synthetic cannabinoids continues to prove challenging to the forensic scientist. As the initially popular compounds, such as JWH-018
More informationSIMULTANEOUS DETERMINATION OF PROCAINE AND BENZOIC ACID BY DERIVATIVE SPECTROMETRY
SIMULTANEOUS DETERMINATION OF PROCAINE AND BENZOIC ACID BY DERIVATIVE SPECTROMETRY Irinel Adriana Badea *, LuminiŃa Vlădescu abstract: A derivative spectrometric has been developed for the determination
More informationA Fast and Sensitive Method for Residual Hydrazine Analysis in Pharmaceutical Samples
A Fast and Sensitive Method for Residual Hydrazine Analysis in Pharmaceutical Samples Vera Leshchinskaya, Priscilla Richberg, Yury Zelechonok*, and Anne Kelly Bristol Myers Squibb Company, P.O.BOX 4000,
More informationBritish American Tobacco Group Research & Development. Method - Determination of phenols in mainstream cigarette smoke
British American Tobacco Group Research & Development Method - Determination of phenols in mainstream cigarette smoke 1 SCOPE OF APPLICATION The method is applicable to the quantitative determination of
More informationLimit-test of NDMA and NDEA in Sartans. by GC-MS (Liquid-direct-injection)
Limit-test of NDMA and NDEA in Sartans 1 Purpose and scope by GC-MS (Liquid-direct-injection) The method is used to test the permissible limits of N-nitrosodimethylamine and N- nitrosodiethylamine in various
More informationTechnical Procedure for Solid Phase Extraction of THC and THC-COOH for GC-MS Analysis
Technical Procedure for Solid Phase Extraction of THC and THC-COOH 1.0 Purpose - This procedure specifies the required elements for the extraction of THC and THC-COOH using United Technologies Styre Screen
More informationXinyu LIU, Peter JOZA, Andrew MASTERS, Bill RICKERT
Determination of Ethylene Oxide (ETO) in Mainstream Cigarette Smoke Using Hydrobromic Acid Derivatization and Gas Chromatography-Mass Spectrometry Method Xinyu LIU, Peter JOZA, Andrew MASTERS, Bill RICKERT
More informationMethod Development and Validation for the Estimation of Darunavir in Rat Plasma by RP-HPLC
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationSimultaneous, Fast Analysis of Melamine and Analogues in Pharmaceutical Components Using Q Exactive - Benchtop Orbitrap LC-MS/MS
Simultaneous, Fast Analysis of Melamine and Analogues in Pharmaceutical Components Using Q Exactive - Benchtop Orbitrap LC-MS/MS Kate Comstock, Tim Stratton, Hongxia (Jessica) Wang, and Yingying Huang
More informationVMA, HVA, 5-HIAA Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 11 VMA, HVA, 5-HIAA Urine LC-MS/MS Analysis Kit User Manual ZV-3030-0200-20 200 2-8 C Page 2 / 11 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE... 3
More informationMulti-residue analysis of pesticides by GC-HRMS
An Executive Summary Multi-residue analysis of pesticides by GC-HRMS Dr. Hans Mol is senior scientist at RIKILT- Wageningen UR Introduction Regulatory authorities throughout the world set and enforce strict
More informationGas Chromatography. 1. Experiment Category: 2. Experiment Name: 3. Date and Issue number: 4. Instructor Name: 5. Institution: Ain Shams University
Project Title: e-laboratories for Gas chromatography 1. Experiment Category: Chemistry >> chromatography 2. Experiment Name: Gas Chromatography 3. Date and Issue number: 4. Instructor Name: 5. Institution:
More informationUniversity of the Aegean
University of the Aegean Department of Environmental Studies Water and Air Quality Laboratory Simultaneous determination of pharmaceutical compounds and endocrine disrupting chemicals (EDCs) in wastewater
More informationLow-level Determination of 4-Hydrazino Benzoic Acid in Drug Substance by High Performance Liquid Chromatography/Mass Spectrometry
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2010, 7(2), 403-408 Low-level Determination of 4-Hydrazino Benzoic Acid in Drug Substance by High Performance Liquid Chromatography/Mass
More informationDefinitive EtG/EtS LC-MS/MS Analysis:
Definitive / LC- Analysis: A Rugged 4-Min Method for High-Throughput Labs By Justin Steimling and Frances Carroll Abstract Methods for monitoring alcohol consumption biomarkers and are generally limited
More informationAnalysis of Geosmin and 2-Methylisoborneol Utilizing the Stratum PTC and Aquatek 70
Analysis of Geosmin and 2-Methylisoborneol Utilizing the Stratum PTC and Aquatek 70 Application Note Abstract Geosmin and 2-Methylisoborneol are organic compounds that have a distinct scent. These compounds
More informationSAMHSA-Compliant LC/MS/MS Analysis of Phencyclidine in Urine with Agilent Bond Elut Plexa PCX and Agilent Poroshell 120
SAMHSA-Compliant LC/MS/MS Analysis of Phencyclidine in Urine with Agilent Bond Elut Plexa PCX and Agilent Poroshell 120 Application Note Forensic Toxicology Authors Irina ioumaeva, John M. Hughes Agilent
More informationImproved Extraction of THC and its Metabolites from Oral Fluid Using Oasis PRiME HLB Solid Phase Extraction (SPE) and a UPLC CORTECS C 18
Using Oasis PRiME HLB Solid Phase Extraction (SPE) and a UPLC CORTECS C 18 Column Xin Zhang, Jonathan P. Danaceau, and Erin E. Chambers Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Semi-validated
More informationAnalysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research
Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research Heather A Brown, 1 Claudia Rossi, 2 and Lisa J Calton 1 1 Waters Corporation, Wilmslow, UK 2
More informationTest method for the determination of NDMA and NDEA by LC-MS/MS in Sartan containing film coated tablets
Test method for the determination of NDMA and NDEA by LC-MS/MS in Sartan containing film coated tablets Contact: Oliver el-atma Chemisches und Veterinäruntersuchungsamt (CVUA) Karlsruhe, Germany (OMCL
More informationProfiling of Diferulates (Plant Cell Wall Cross- Linkers) Using Ultrahigh-performance Liquid. Chromatography-Tandem Mass Spectrometry
Supporting Information for: Profiling of Diferulates (Plant Cell Wall Cross- Linkers) Using Ultrahigh-performance Liquid Chromatography-Tandem Mass Spectrometry Ramin Vismeh a,b, Fachuang Lu c,d, Shishir
More informationMETHOD 8033 ACETONITRILE BY GAS CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION
METHOD 80 ACETONITRILE BY GAS CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION 1.0 SCOPE AND APPLICATION 1.1 Method 80 may be used to determine the concentration of acetonitrile (CAS No. 75-05-8) in aqueous
More informationAgilent 1290 Infinity Quaternary LC Stepwise Transfer to Methods with MS-Compatible Mobile Phases
Agilent 129 Infinity Quaternary LC Stepwise Transfer to Methods with MS-Compatible Mobile Phases Technical Overview Author A.G.Huesgen Agilent Technologies, Inc. Waldbronn, Germany Abstract The Agilent
More informationSimultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS
Simultaneous analysis for forensic drugs in human blood and urine using ultra-high speed LC-MS/MS TIAFT 204 Toshikazu Minohata, Keiko Kudo 2, Kiyotaka Usui 3, Noriaki Shima 4, Munehiro Katagi 4, Hitoshi
More informationDrugs of Abuse by Direct Sample Probe Using Single Quadrupole GC/MS
Application Note: 10018 Drugs of Abuse by Direct Sample Probe Using Single Quadrupole GC/MS Thermo Fisher Scientific, Austin, TX, USA Key Words Direct Sample Probe Single Quadrupole GC/MS Drugs of Abuse
More informationTHE NEW QUANTITATIVE ANALYTICAL METHOD FOR ULTRATRACE SULFUR COMPOUNDS IN NATURAL GAS
International Gas Union Research Conference 14 THE NEW QUANTITATIVE ANALYTICAL METHOD FOR ULTRATRACE SULFUR COMPOUNDS IN NATURAL GAS Main author Hironori IMANISHI Tokyo Gas Co., Ltd. JAPAN himanishi@tokyo-.co.jp
More informationThe Analysis of Trace Contaminants in High Purity Ethylene and Propylene Using GC/MS. Application. Agilent Technologies/Wasson ECE Monomer Analyzer
The Analysis of Trace Contaminants in High Purity Ethylene and Propylene Using GC/MS Agilent Technologies/Wasson ECE Monomer Analyzer Application Authors Fred Feyerherm 119 Forest Cove Dr. Kingwood, TX
More information