Chapter 8 Assessment (Take Home) AP Biology

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1 Chapter 8 Assessment (Take Home) AP Biology 1) In autotrophic bacteria, where is chlorophyll located? A) in chloroplast membranes B) in chloroplast stroma C) in the ribosomes D) in the nucleoid E) in the infolded plasma membrane 2) Which of the following are products of the light reac ons of photosynthesis that are u lized in the Calvin cycle? A) CO 2 and glucose B) H 2 O and O 2 C) ADP, i, and NADP + D) electrons and H + E) ATP and NADPH 3) Where does the Calvin cycle take place? A) stroma of the chloroplast B) thylakoid membrane C) cytoplasm surrounding the chloroplast D) interior of the thylakoid (thylakoid space) E) outer membrane of the chloroplast 4) When oxygen is released as a result of photosynthesis, it is a direct by-product of A) reducing NADP +. B) spli ng water molecules. C) chemiosmosis. D) the electron transfer system of photosystem I. E) the electron transfer system of photosystem II. 5) In the thylakoid membranes, what is the main role of the pigment molecules in a light-harves ng complex? A) split water and release oxygen to the reac on-center chlorophyll B) transfer light energy to the reac on-center chlorophyll C) synthesize ATP from ADP and i D) transfer electrons to ferredoxin and then NADPH E) concentrate photons within the stroma 6) Which of the following events occurs in the light reac ons of photosynthesis? A) NADP is produced. B) NADPH is reduced to NADP+. C) Carbon dioxide is incorporated into PGA. D) ATP is phosphorylated to yield ADP. E) Light is absorbed and funneled to reac on-center chlorophyll a.

2 7) Which statement describes the func oning of photosystem II? A) Light energy excites electrons in the thylakoid membrane electron transport chain. B) Photons are passed along to a reac on-center chlorophyll. C) The P680 chlorophyll donates a pair of protons to NADP+, which is thus converted to NADPH. D) The electron vacancies in P680+ are filled by electrons derived from water. E) The spli ng of water yields molecular carbon dioxide as a by-product. 8) Which of the following are directly associated with photosystem I? A) harves ng of light energy by ATP B) receiving electrons from the thylakoid membrane electron transport chain C) genera on of molecular oxygen D) extrac on of hydrogen electrons from the spli ng of water E) passing electrons to the thylakoid membrane electron transport chain 9) Some photosynthe c organisms contain chloroplasts that lack photosystem II, yet are able to survive. The best way to detect the lack of photosystem II in these organisms would be A) to determine if they have thylakoids in the chloroplasts. B) to test for libera on of O 2 in the light. C) to test for CO 2 fixa on in the dark. D) to do experiments to generate an ac on spectrum. E) to test for produc on of either sucrose or starch. 10) What does the chemiosmo c process in chloroplasts involve? A) establishment of a proton gradient across the thylakoid membrane B) diffusion of electrons through the thylakoid membrane C) reduc on of water to produce ATP energy D) movement of water by osmosis into the thylakoid space from the stroma E) forma on of glucose, using carbon dioxide, NADPH, and ATP 11) Suppose the interior of the thylakoids of isolated chloroplasts were made acidic and then transferred in the dark to a ph 8 solu on. What would be likely to happen? A) The isolated chloroplasts will make ATP. B) The Calvin cycle will be ac vated. C) Cyclic photophosphoryla on will occur. D) The isolated chloroplasts will generate oxygen gas. E) The isolated chloroplasts will reduce NADP+ to NADPH. 12) In a plant cell, where are the ATP synthase complexes located? A) thylakoid membrane only B) plasma membrane only C) inner mitochondrial membrane only D) thylakoid membrane and inner mitochondrial membrane E) thylakoid membrane and plasma membrane

3 13) Which of the following statements best describes the rela onship between photosynthesis and respira on? A) Respira on runs the biochemical pathways of photosynthesis in reverse. B) Photosynthesis stores energy in complex organic molecules, whereas respira on releases it. C) Photosynthesis occurs only in plants and respira on occurs only in animals. D) ATP molecules are produced in photosynthesis and used up in respira on. E) Respira on is anabolic and photosynthesis is catabolic. 14) Where are the molecules of the electron transport chain found in plant cells? A) thylakoid membranes of chloroplasts B) stroma of chloroplasts C) outer membrane of mitochondria D) matrix of mitochondria E) cytoplasm 15) The spli ng of carbon dioxide to form oxygen gas and carbon compounds occurs during A) photosynthesis. B) respira on. C) both photosynthesis and respira on. D) neither photosynthesis nor respira on. E) photorespira on. 16) Genera on of proton gradients across membranes occurs during A) photosynthesis. B) respira on. C) both photosynthesis and respira on. D) neither photosynthesis nor respira on. E) photorespira on. 17) P680+ is said to be the strongest biological oxidizing agent. Given its func on, why is this necessary? A) It is the receptor for the most excited electron in either photosystem. B) It is the molecule that transfers electrons to plastoquinone (Pq) of the electron transfer system. C) It transfers its electrons to reduce NADP+ to NADPH. D) It obtains electrons from the oxygen atom in a water molecule, so it must have a stronger a rac on for electrons than oxygen has. E) It has a posi ve charge. 18) Some photosynthe c bacteria (e.g., purple sulfur bacteria) have only photosystem I, whereas others (e.g., cyanobacteria) have both photosystem I and photosystem II. Which of the following might this observa on imply? A) Photosystem II was selected against in some species. B) Photosynthesis with only photosystem I is more ancestral. C) Photosystem II may have evolved to be more photoprotec ve. D) Linear electron flow is more primi ve than cyclic flow of electrons. E) Cyclic flow is more necessary than linear electron flow.

4 19) The reac ons that produce molecular oxygen (O 2 ) take place in A) the light reac ons alone. B) the Calvin cycle alone. C) both the light reac ons and the Calvin cycle. D) neither the light reac ons nor the Calvin cycle. E) the chloroplast, but are not part of photosynthesis. 20) The ph of the inner thylakoid space has been measured, as have the ph of the stroma and of the cytosol of a par cular plant cell. Which, if any, rela onship would you expect to find? A) The ph within the thylakoid is less than that of the stroma. B) The ph of the stroma is lower than that of the other two measurements. C) The ph of the stroma is higher than that of the thylakoid space but lower than that of the cytosol. D) The ph of the thylakoid space is higher than that anywhere else in the cell. E) There is no consistent rela onship. 21) What compound provides the reducing power for the Calvin cycle reac ons? A) ATP B) NAD+ C) NADH D) NADP+ E) NADPH 22) What would be the expected effect on plants if the atmospheric CO 2 concentra on was doubled? A) All plants will experience increased rates of photosynthesis. B) C3 plants will have faster growth; C4 plants will be minimally affected. C) C4 plants will have faster growth; C3 plants will be minimally affected. D) C3 plants will have faster growth; C4 plants will have slower growth. E) Plant growth will not be affected because atmospheric CO 2 concentra ons are never limi ng for plant growth. 23) Where do the enzyma c reac ons of the Calvin cycle take place? A) stroma of the chloroplast B) thylakoid membranes C) matrix of the mitochondria D) cytosol around the chloroplast E) thylakoid space 24) What is the primary func on of the Calvin cycle? A) use ATP to release carbon dioxide B) use NADPH to release carbon dioxide C) split water and release oxygen D) transport RuBP out of the chloroplast E) synthesize simple sugars from carbon dioxide

5 25) Which of the following statements best represents the rela onships between the light reac ons and the Calvin cycle? A) The light reac ons provide ATP and NADPH to the Calvin cycle, and the cycle returns ADP, i, and NADP+ to the light reac ons. B) The light reac ons provide ATP and NADPH to the carbon fixa on step of the Calvin cycle, and the cycle provides water and electrons to the light reac ons. C) The light reac ons supply the Calvin cycle with CO 2 to produce sugars, and the Calvin cycle supplies the light reac ons with sugars to produce ATP. D) The light reac ons provide the Calvin cycle with oxygen for electron flow, and the Calvin cycle provides the light reac ons with water to split. E) There is no rela onship between the light reac ons and the Calvin cycle. 26) In the process of carbon fixa on, RuBP a aches a CO 2 to produce a six-carbon molecule, which is then split to produce two molecules of 3-phosphoglycerate. A er phosphoryla on and reduc on produces glyceraldehyde 3-phosphate (G3P), what more needs to happen to complete the Calvin cycle? A) addi on of a pair of electrons from NADPH B) inac va on of RuBP carboxylase enzyme C) regenera on of ATP from ADP D) regenera on of RuBP E) regenera on of NADP+ 27) Photorespira on occurs when rubisco reacts RuBP with A) CO 2 B) O 2 C) glyceraldehyde 3-phosphate. D) 3-phosphoglycerate. E) NADPH. 28) CAM plants keep stomata closed in day me, thus reducing loss of water. They can do this because they A) fix CO 2 into organic acids during the night. B) fix CO 2 into sugars in the bundle-sheath cells. C) fix CO 2 into pyruvate in the mesophyll cells. D) use the enzyme phosphofructokinase, which outcompetes rubisco for CO 2. E) use photosystem I and photosystem II at night.

6 Figure ) Figure 8.1 shows the absorp on spectrum for chlorophyll a and the ac on spectrum for photosynthesis. Why are they different? A) Green and yellow wavelengths inhibit the absorp on of red and blue wavelengths. B) Bright sunlight destroys photosynthe c pigments. C) Oxygen given off during photosynthesis interferes with the absorp on of light. D) Other pigments absorb light in addi on to chlorophyll a. E) Aerobic bacteria take up oxygen, which changes the measurement of the rate of photosynthesis. 30) What wavelength of light in Figure 8.1 is most effec ve in driving photosynthesis? A) 420 mm B) 475 mm C) 575 mm D) 625 mm E) 730 mm

7 Figure ) If ATP used by this plant is labeled with radioac ve phosphorus, which molecule or molecules of the Calvin cycle will be radioac vely labeled first? A) B only B) B and C only C) B, C, and D only D) B and E only E) B, C, D, and E 32) If the carbon atom of each of the incoming CO 2 molecules is labeled with a radioac ve isotope of carbon, which organic molecules will be radioac vely labeled a er one cycle? A) C only B) B, C, D, and E C) C, D, and E only D) B and C only E) B and D only

8 33) To iden fy the molecule that accepts CO 2, Calvin and Benson manipulated the carbon-fixa on cycle by either cu ng off CO 2 or cu ng off light from cultures of photosynthe c algae. They then measured the concentra ons of various metabolites immediately following the manipula on. How would these experiments help iden fy the CO 2 acceptor? Study Figure 8.2 to help you in determining the correct answer. A) The CO 2 acceptor concentra on would decrease when either the CO 2 or light is cut off. B) The CO 2 acceptor concentra on would increase when either the CO 2 or light is cut off. C) The CO 2 acceptor concentra on would increase when the CO 2 is cut off, but decrease when the light is cut off. D) The CO 2 acceptor concentra on would decrease when the CO 2 is cut off, but increase when the light is cut off. E) The CO 2 acceptor concentra on would stay the same regardless of the CO 2 or light. Theodor W. Engelmann illuminated a filament of algae with light that passed through a prism, thus exposing different segments of algae to different wavelengths of light. He added aerobic bacteria and then noted in which areas the bacteria congregated. He noted that the largest groups were found in the areas illuminated by the red and blue light. 34) What did Engelmann conclude about the congrega on of bacteria in the red and blue areas? A) Bacteria released excess carbon dioxide in these areas. B) Bacteria congregated in these areas due to an increase in the temperature of the red and blue light. C) Bacteria congregated in these areas because these areas had the most oxygen being released. D) Bacteria are a racted to red and blue light and thus these wavelengths are more reac ve than other wavelengths. E) Bacteria congregated in these areas due to an increase in the temperature caused by an increase in photosynthesis. 35) An outcome of this experiment was to help determine the rela onship between A) heterotrophic and autotrophic organisms. B) wavelengths of light and the rate of aerobic respira on. C) wavelengths of light and the amount of heat released. D) wavelengths of light and the rate of photosynthesis. E) the concentra on of carbon dioxide and the rate of photosynthesis. 36) If you ran the same experiment without passing light through a prism, what would you predict? A) There would be no difference in results. B) The bacteria would be rela vely evenly distributed along the algal filaments. C) The number of bacteria present would decrease due to an increase in the carbon dioxide concentra on. D) The number of bacteria present would increase due to an increase in the carbon dioxide concentra on. E) The number of bacteria would decrease due to a decrease in the temperature of the water.

9 37) A gardener is concerned that her greenhouse is ge ng too hot from too much light, and seeks to shade her plants with colored translucent plas c sheets, the color of which allows passage of only that wavelength. What color should she use to reduce overall light energy, but s ll maximize plant growth? A) green B) blue C) yellow D) orange E) Any color will work equally well. 38) The light reac ons of photosynthesis supply the Calvin cycle with A) light energy. B) CO2 and ATP. C) H2O and NADPH. D) ATP and NADPH. E) sugar and O2. 39) Which of the following sequences correctly represents the flow of electrons during photosynthesis? A) NADPH O 2 CO 2 B) H 2 O NADPH Calvin cycle C) NADPH chlorophyll Calvin cycle D) H 2 O photosystem I photosystem II E) NADPH electron transport chain O 2 40) How is photosynthesis similar in C4 plants and CAM plants? A) In both cases, electron transport is not used. B) Both types of plants make sugar without the Calvin cycle. C) In both cases, rubisco is not used to fix carbon ini ally. D) Both types of plants make most of their sugar in the dark. E) In both cases, thylakoids are not involved in photosynthesis. 41) Which of the following statements is a correct dis nc on between autotrophs and heterotrophs? A) Only heterotrophs require chemical compounds from the environment. B) Cellular respira on is unique to heterotrophs. C) Only heterotrophs have mitochondria. D) Autotrophs, but not heterotrophs, can nourish themselves beginning with CO2 and other nutrients that are inorganic. E) Only heterotrophs require oxygen. 42) Which of the following does not occur during the Calvin cycle? A) carbon fixa on B) oxida on of NADPH C) release of oxygen D) regenera on of the CO 2 acceptor E) consump on of ATP 43) In mechanism, photophosphoryla on is most similar to A) substrate-level phosphoryla on in glycolysis. B) oxida ve phosphoryla on in cellular respira on. C) the Calvin cycle. D) carbon fixa on. E) reduc on of NADP+. 44) Which process is most directly driven by light energy? A) crea on of a ph gradient by pumping protons across the thylakoid membrane B) carbon fixa on in the stroma C) reduc on of NADP+ molecules D) removal of electrons from chlorophyll molecules E) ATP synthesis

10 Color Wavelength (nm) Violet Blue Cyan Green Yellow Orange Red ) An absorp on spectrum indicates the rela ve amount of light absorbed across a range of wavelengths. The graphs above represent the absorp on spectra of individual pigments isolated from two different organisms. One of the pigments is chlorophyll a, commonly found in green plants. The other pigment is bacteriorhodopsin, commonly found in purple photosynthe c bacteria. The table above shows the approximate ranges of wavelengths of different colors in the visible light spectrum. (a) Identify the pigment (chlorophyll a or bacteriorhodopsin) used to generate the absorp on spectrum in each of the graphs above. Explain and justify your answer. (b) In an experiment, iden cal organisms containing the pigment from Graph II as the predominant lightcapturing pigment are separated into three groups. The organisms in each group are illuminated with light of a single wavelength (650 nm for the first group, 550 nm for the second group, and 430 nm for the third group). The three light sources are of equal intensity, and all organisms are illuminated for equal lengths of me. Predict the rela ve rate of photosynthesis in each of the three groups. Justify your predic ons.

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