DNA Structure and Function
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1 DNA Structure and Function
2 Nucleotide Structure 1. 5-C sugar RNA ribose DNA deoxyribose 2. Nitrogenous Base N attaches to 1 C of sugar Double or single ring Four Bases Adenine, Guanine, Thymine, Cytosine 3. Phosphate Attached to 5 C of sugar
3 DNA Information center of the cell Particular sequence of nucleotide bases forms a gene Gene codes for proteins Before a protein is made, genes must be transcribed into RNA
4 Fig Copyright 2002 Pearson Education, Inc., publishing as Benjamin Cummings
5 DNA Replication Semi-Conservative Model Each parent strand remains intact Every DNA molecule is half old and half new Errors in DNA replication can create mutations new old old new 5 3
6 Chromosome Structure Chromatin relaxed form of genetic material is necessary during protein synthesis Chromosomes condensed form of genetic material necessary during cell division
7 Overview transcription of protein mrna rrna trna synthesis Transcription: Copy of DNA is transcribed as mrna Translation: Structures called ribosomes assemble amino acid sequence according to the code on the mrna translation mature mrna transcripts ribosomal subunits mature trna
8 Gene Mutations Base-pair substitutions Insertions Deletions
9 Base-Pair Substitution original base triplet in a DNA strand a base substitution within the triplet (red) During replication, proofreading enzymes make a substitution. possible outcomes: or original, unmutated sequence a gene mutation
10 Frameshift Mutations Insertion Extra base added into gene region Deletion Base removed from gene region Both shift the reading frame Result in altered amino acid sequence
11 Frameshift Mutation mrna parental DNA arginine glycine tyrosine tryptophan asparagine amino acids arginine glycine leucine leucine glutamate altered mrna DNA with base insertion altered aminoacid sequence
12 Mutations Each gene has a characteristic mutation rate Natural and synthetic chemicals, and radiation, increase mutation rate Only mutations that arise in germ cells can pass on to next generation Important evolutionary consequences
13 Division Mechanisms Eukaryotic organisms Mitosis Meiosis Prokaryotic organisms Prokaryotic fission
14 Roles of Mitosis Multicelled organisms Growth Cell replacement Some protistans, fungi, plants, animals Asexual reproduction
15 Chromosome A DNA molecule and attached proteins Duplicated in preparation for cell division one chromosome (unduplicated) one chromosome (duplicated)
16 Chromosome Number Total number of chromosomes in cell Germ cells are diploid (2n) Gametes are haploid (n) Meiosis halves chromosome number
17 Human Chromosome Number Diploid chromosome number (n) = 46 Two sets of 23 chromosomes One set from father One set from mother Mitosis produces cells with 46 chromosomes: two of each type
18 Maintaining chromosomes (unduplicated) in parent cell at interphase Chromosome Number same chromosomes (duplicated) in interphase prior to mitosis mitosis, cytoplasmic division chromosome (unduplicated) in daughter cell at interphase chromosome (unduplicated) in daughter cell at interphase
19 Asexual Reproduction Single parent produces offspring All offspring are genetically identical to one another and to parent
20 Sexual Reproduction Involves Meiosis Gamete production Fertilization Produces genetic variation among offspring
21 Homologous Chromosomes Carry Different Alleles Cell has two of each chromosome Chromosome pairs: one from mother, one from father Paternal and maternal chromosomes carry different alleles
22 Sexual Reproduction Shuffles Alleles Through sexual reproduction, offspring inherit new combinations of alleles, which lead to variations in traits Variation in traits is the basis for evolutionary change
23 Gamete Formation Gametes are sex cells (sperm, eggs) Arise from germ cells in reproductive organs ovaries testes
24 Diploid To Haploid centromere one chromatid its sister chromatid one chromosome in the duplicated state
25 each homologue in the cell pairs with its partner then partners separate two chromosomes (unduplicated) one chromosome (duplicated) Stepped Art p.103
26 Mitosis & Meiosis Compared Mitosis Functions Asexual reproduction Growth, repair Occurs in somatic cells Produces clones Function Meiosis Sexual reproduction Occurs in germ cells Produces variable offspring
27 Haploid to Diploid Gametophyte Haploid gamete producing body Sporophyte Diploid product of fused gametes Spore Resting structure
28 GENETICS: Observing Patterns in Inherited Traits
29 Genes Units of information about specific traits Passed from parents to offspring Each has a specific location (locus) on a chromosome
30 Alleles Different molecular forms of a gene found on homologous chromosomes Arise by mutation Dominant allele masks a recessive allele that is paired with it
31 Allele Combinations Homozygous having two identical alleles Homozygous dominant, AA Homozygous recessive, aa Heterozygous having two different alleles Aa
32 Genotype & Phenotype Genotype refers to particular genes an individual carries (RR or Rr or rr) Phenotype refers to an individual s observable traits (flower color, seed shape, etc)
33 Other Definitions Dominant allele in a heterozygous individual, a trait that is fully expressed in the phenotype Recessive allele in a heterozygous individual, a trait that is completely masked by the expression of the dominant allele Pure (true) breeding a population with only one type of allele for a given trait Self cross when individuals of a generation fertilize themselves (e.g., self-fertilized flower).
34 Chromosomes A pair of homologous chromosomes, each in the unduplicated state (most often, one from a male parent and its partner from a female parent) A gene locus (plural, loci), the location for a specific gene on a specific type of chromosome A pair of alleles (each being a certain molecular form of a gene) at corresponding loci on a pair of homologous chromosomes Three pairs of genes (at three loci on this pair of homologous chromosomes); same thing as three pairs of alleles Fig. 8-1, p.113
35 Gregor Mendel Father of Genetics Austrian Monk Strong background in mathematics observed evidence of how parents transmit genes to offspring Unaware of cells, chromosomes or genes Fig. 10-2, p.152
36 F 1 Results of One Monohybrid Cross True-breeding homozygous recessive parent plant F 1 PHENOTYPES aa True-breeding homozygous dominant parent plant a a Aa Aa A Aa Aa AA A Aa Aa Aa Aa Fig. 10-7b1, p.155
37 Monohybrid Cross Experimental intercross between two F 1 heterozygotes AA X aa Aa (F 1 monohybrids) Aa X Aa?
38 True-breeding homozygous recessive parent plant F1 PHENOTYPES A aa Monohybrid Cross True-breeding homozygous dominant parent plant AA A A a Aa Aa a Aa Aa Aa Aa Aa Aa An F1 plant self-fertilizes and produces gametes: Aa F2 PHENOTYPES AA Aa A a A AA Aa a Aa aa Aa aa
39 Dominant Form Recessive Form FLOWER COLOR 705 purple 224 white 3.15:1 FLOWER POSITION 651 along stem 207 at tip 3.14:1 STEM LENGTH 787 tall 227 dwarf Average F 2 dominant-to-recessive ratio for all of the traits studied: 2.84:1 3:1 Fig. 8-5, p.115
40 Dihybrid Cross AB X ab Experimental cross between individuals that are homozygous for different versions of two traits
41 Dihybrid Cross: F 1 Results TRUE- BREEDING PARENTS: purple flowers, tall white flowers, dwarf AABB x aabb GAMETES: AB AB ab ab AaBb F1 HYBRID OFFSPRING: all purple-flowered, tall
42 Dihybrid Cross: F 2 Results AaBb X AaBb 1/4 AB 1/4 Ab 1/4 ab 1/4 ab 1/4 AB 1/16 AABB 1/16 AABb 1/16 AaBB 1/16 AaBb 9/16 purple-flowered, tall 3/16 purple-flowered, dwarf 1/4 Ab 1/16 AABb 1/16 AAbb 1/16 AaBb 1/16 Aabb 3/16 white-flowered, tall 1/16 white-flowered, dwarf 1/4 ab 1/16 AaBB 1/16 AaBb 1/16 aabb 1/16 aabb 1/4 ab 1/16 AaBb 1/16 Aabb 1/16 aabb 1/16 aabb
43 Dominance Relations Complete dominance Incomplete dominance Codominance
44 Codominance: ABO Blood Types Gene that controls ABO type codes for enzyme that determines structure of a glycolipid on blood cells Two alleles (I A and I B ) are codominant when paired Third allele (i) is recessive to others
45 ABO Blood Type: A Multiple Allele System Range of genotypes: I A I A or I B I B or I A i I A I B I B i ii Blood types: A AB B O
46 Fig Copyright 2002 Pearson Education, Inc., publishing as Benjamin Cummings
47 Incomplete Dominance homozygous parent Incomplete X Dominance homozygous parent All F 1 are heterozygous F 2 shows three phenotypes in 1:2:1 ratio X
48 Gene interactions and phenotypic expression Genes may interact with each other: one gene influences phenotypic expression of others Complex variations: phenotype influenced by gene interactions and/or environmental conditions
49 Interactions among Gene Pairs Common among genes for hair color in mammals BLACK LABRADOR YELLOW LABRADOR CHOCOLATE LABRADOR
50 Genetics of Coat Color in Labrador Retrievers Epistasis: Phenotypic expression of one gene is governed by another Two genes involved - One gene influences melanin production Two alleles - B (black) is dominant over b (brown) - Other gene influences melanin deposition Two alleles - E promotes pigment deposition and is dominant over e
51 Black color dominant B & E must be present Yellow color recessive e & either B or b Chocolate color dominant E & recessive b
52 Continuous Variation A continuous range of small differences in a given trait among individuals The greater the number of genes and environmental factors that affect a trait, the more continuous the variation in that trait
53 Controlled by more than one gene Two genes A or a and B or b dark brown 4 dominants medium brown 3 dominants light brown or hazel 2 dominants dark blue, grey or green 1 dominants Fig , p.160 light blue 0 dominants
54 Continuous Variation Skin Color in humans: three genes with multiple alleles Fig Copyright 2002 Pearson Education, Inc., publishing as Benjamin Cummings
55 Environmental Effects on Phenotype Genotype and environment can interact to affect phenotype Himalayan rabbit ice pack experiment Transplantation of plant cuttings to different elevations
56 Environmental Effects on Phenotype
57 Phenotypic Plasticity Phenotype change in response to the environment. Examples: Humans tan in response to sun exposure; increased melanin protects cells from harmful solar radiation Mussels exposed to seastar scents develop stronger adductor muscles Mussels exposed to dog whelk scent develop thicker shells
58 Phenotypic Plasticity Phenotype change in response to the environment. Predator mediated
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