Chapter 2 Cells and Cell Division
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1 Chapter 2 Cells and Cell Division MULTIPLE CHOICE 1. The process of meiosis results in: A. the production of four identical cells B. no change in chromosome number from parental cells C. a doubling of the chromosome number D. a reduction in chromosome number E. two interphase cells D 2. In the cell cycle, the G1 phase represents: A. the time of DNA synthesis B. splitting of the chromosomes into chromatids C. a period of growth D. the stage just prior to mitosis E. the stage just prior to meiosis C 3. Ribosomes are organelles that function in: A. separation of chromosomes by spindle fibers B. cellular energy production C. synthesis of gene products D. transport of materials throughout the cytoplasm E. conversion of chromatin into chromosomes C 4. Which of the following genetic diseases involve defects in DNA repair, which affect cell division? A. Gaucher disease and Werner syndrome B. Kearns-Sayre syndrome and Progeria C. Progeria and Werner syndrome D. Gaucher disease and Cystic fibrosis E. Progeria and Werner syndrome E 5. For cells grown in the laboratory, the largest part of the cell cycle is: A. Interphase. B. Mitosis. C. Cytokinesis. D. They are all about equal. E. There is no rule. A
2 6. Pieces of what are exchanged during crossing-over? A. sister chromatids B. the maternal and paternal chromosomes C. centromeres D. a chromatid of the maternal chromosome and a chromatid of the paternal chromosome E. X and Y chromosome D 7. If an organism has a diploid chromosome number of 4, how many chromosome pairs will be visible in meiosis? A. 0 B. 2 C. 4 D. 6 E. 8 B 8. Autosomes represent: A. all chromosomes including the sex chromosomes B. the half of the chromosomes inherited from one parent C. all chromosomes other than the sex chromosomes D. chromosome pairs with unlike members E. those chromosomes found only in gametes C 9. During meiosis in an organism where 2n = 6, how many chromatids will be present in a cell at the beginning of meiosis II? A. 2 B. 4 C. 6 D. 8 E. 12 C 10. The Hayflick limit describes: A. the size limit to which a cell can grow B. the number of divisions a cultured cell can undergo C. the largest number of chromosomes an organism can possess D. the ratio of nucleus to cytoplasm E. none of these B 11. In meiosis, the centromeres divide at: A. metaphase I B. anaphase I C. metaphase II D. anaphase II E. telophase D
3 12. Haploid gametes contain: A. the 2n chromosome number B. half the 2n chromosome number C. twice the 2n chromosome number D. half the n chromosome number E. none of these B 13. Endoplasmic reticulum is involved in: A. energy production B. transport of materials throughout the cell C. ribosome synthesis D. carrying genetic information E. generation of polar bodies B 14. A cell that could not form spindle fibers could not perform: A. mitosis B. meiosis C. mitosis or meiosis D. DNA replication E. protein synthesis C 15. Gaucher disease is associated with abnormality of which part of the cell? A. endoplasmic reticulum B. lysosomes C. mitochondria D. Golgi apparatus E. plasma membrane B 16. Molecules on or in a cell's plasma membrane are responsible for: A. transport of substances into the cell B. providing a molecular identity for the cell C. determining blood type D. all of these E. none of these D 17. Which of the following is the first event that occurs in prophase of mitosis? A. The chromosomes are duplicated. B. The nuclear envelope starts to break up. C. The mitotic spindle begins to form. D. The chromosomes begin to condense. E. The cleavage furrow forms and deepens. D
4 18. A cell in G 0 state is a cell: A. that will shortly enter G 1 B. that never divides C. that has just finished mitosis but has not yet begun cytokinesis D. in cytokinesis E. just after cytokinesis B 19. Centromeres: A. organize microtubules into spindle fibers B. connect sister chromatids C. attach chromosomes to spindle fibers D. both connect sister chromatids and attach chromosomes to spindle fibers E. all of the answers are correct D 20. Which of the following are NOT haploid? A. polar bodies and secondary spermatocytes B. primary oocytes and spermatids C. secondary spermatocytes and spermatogonia D. primary oocytes and spermatogonia E. secondary spermatocytes and spermatids D 21. Formation of eggs and sperm are the same in what way(s)? A. when during a person's life they begin and end B. the way chromosomes behave during meiosis C. the way cytoplasm divides during cytokinesis D. both the way chromosomes behave during meiosis and the way cytoplasm divides during cytokinesis E. all of these B 22. Which of the following types of molecules serve primarily as energy sources or reserves? A. carbohydrates B. lipids C. proteins D. carbohydrates and lipids E. carbohydrates and proteins D 23. Which of the following is not a lipid? A. fats B. steroids C. oils D. monosaccharides E. phospholipids D
5 TRUE/FALSE 1. The enzymes present in lysosomes must previously have been in the Golgi apparatus and endoplasmic reticulum. T 2. Skin cells typically do not divide. F 3. Chromatin is the term for a chromosome before it is duplicated. F 4. Mitotic divisions reduce the number of chromosomes found in daughter cells. F 5. Cytokinesis usually occurs just after mitosis. T 6. Autosomal chromosome pairs are identical, whereas the sex chromosome pair in males is not. T 7. "Random Assortment" is partially responsible for our genetic diversity. T 8. Crossing over occurs between chromatids of chromosome pairs. T 9. There are 92 chromosomes in a normal human cell undergoing mitosis at the anaphase stage. T 10. The S stage of interphase is followed immediately by mitosis. F 11. A polar body, once formed, has no further function and dies. T 12. The molecules on the surface of cells that give cells their identity are combinations of carbohydrates and proteins. T
6 COMPLETION 1. How many autosomes are present in a human egg? is a disease that occurs when cells escape from controls that are part of the cell cycle. cancer 3. What is the chromosomal structure that anchors the spindle fiber to the chromosome? the centromere 4. What occurs during "S" phase of the cell cycle? DNA duplication 5. In mitosis, chromatids separate and move to opposite poles of the spindle during. anaphase 6. In many respects, the events of telophase seem to be the reverse of those occurring in. prophase 7. In meiosis, sister chromatids separate and move to opposite poles of the spindle during. anaphase II 8. In cell division, toward the end of nuclear division, the divides by a process called cytokinesis to produce two identical cells. cytoplasm 9. The only cytoplasmic organelles that contain DNA are the. mitochondria 10. Ribosomes exist either free in the cytoplasm or attached to the membranes of. endoplasmic reticulum
7 11. One primary spermatocyte produces (how many?) functional sperm(s); one primary oocyte produces functional egg(s). 4; 1 four; one 12. Proteins are polymers made of subunits called. amino acids 13. Mostly proteins are insoluble in water and provide structure of hair, skin, and bones. fibrous SHORT ANSWER 1. Since in most specialized cells of the body only a relatively small number of genes is active, why must mitosis involve the replication of a complete set of genes? Mitosis replicates and divides whole chromosomes. Since the genes active in any type of cell are scattered among all the chromosomes, every cell needs a complete set of chromosomes. 2. From an evolutionary standpoint, does it seem logical that mitosis evolved before meiosis, and that meiosis is really a specialized form of mitosis? Or should mitosis be regarded as a degenerate form of meiosis? All organisms require cell division and mitosis since all muticellular organisms must grow and replace dead cells and unicellular organisms multiply by going through cell division. Meiosis is only required for sexual reproduction - something the simplest unicellular organisms do not do. Hence it is logical to assume that mitosis evolved first. 3. Would an understanding of the mechanism of the Hayflick limit lead to an increase in the human life span? Yes. The loss of cells ability to divide is one thing that causes the symptoms of old age. If the mechanism of the Hayflick limit were fully understood, it might lead to the ability to extend or abolish the limit and to an increase in life span. 4. What is the difference between life span and life expectancy? Which genetic and non-genetic factors contribute to the gap between life span and life expectancy? Life span is the (theoretical) average age of an organism at death. Life expectancy is the age at which a particular individual or group is likely to die. There are a large number of genetic and non-genetic factors that influence individuals health and safety and thus their life expectancy.
8 5. Compare and contrast the following: a. prophase of mitosis and prophase I of meiosis b. interphase preceding meiosis I and interphase preceding meiosis II c. anaphase of mitosis and anaphase I of meiosis a. The same events occur in both, but only in prophase I of meiosis will homologous chromosomes pair and crossing over occur. b. There is essentially no visible difference; only the number of chromosomes present is different. c. In both cases chromosomes move away from each other. In mitosis, centromeres split and sister chromatids (which become chromosomes) separate. In anaphase I, centromeres do not split and homologous chromosomes which had been paired, still containing two sister chromatids, separate. 6. What evidence exists that mitosis and the cell cycle are under genetic control? Many specific gene mutations which alter the cell cycle and/or events of mitosis have been identified. They are transmitted from parents to offspring in the same manner as any other gene. 7. Of what significance is crossing over? What other event in Meiosis I is of similar significance? Crossing over produces new combinations of maternal and paternal genes, leading to sperm and egg cells, and offspring, with unique sets of genes and traits. The random assortment of homologous chromosomes plays the same role. But while random assortment leads to millions of different genetic combinations, crossing over makes the number of variations possible almost infinite. 8. Describe the cell cycle. Do all cells go through this cycle at the same time? The cell cycle is a genetically controlled series of events during which a cell grows (G1), then its DNA is replicated (S), then other preparations for division occur (G2). Then the cell divides (mitosis and cytokinesis). Different cells divide at different times and their cycles and cycle stages are of different lengths. 9. Compare and contrast five items in mitosis and meiosis. 1. Mitosis produces two diploid daughter cells; meiosis produces four haploid cells. 2. The products of mitosis are somatic cells; the products of meiosis are gametes (or cells that will produce gametes). 3. Mitosis is one division; meiosis is two. 4. Pairing of homologous chromosomes and crossing over occur during meiosis, but not mitosis. 5. Cells that divide by mitosis usually go through the cell cycle and divide again. Cells produced by meiosis do not usually divide again (in animals). 10. What is accomplished by the unequal cytokinesis of oogenesis? Any egg cell needs a certain amount of cytoplasm and nutrients to support the young embryo. Because of unequal cytokinesis, almost all the cytoplasm and nutrients in the oocyte are preserved in the single egg.
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